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Journal of Chemical Ecology

, Volume 40, Issue 6, pp 643–652 | Cite as

Humic Substances Enhance Growth and Respiration in the Basidiomycetes Trametes Maxima Under Carbon Limited Conditions

  • Olga I. Klein
  • Elena P. Isakova
  • Yulia I. Deryabina
  • Natalia A. Kulikova
  • Gennadii A. Badun
  • Maria G. Chernysheva
  • Elena V. Stepanova
  • Olga V. Koroleva
Article

Abstract

Humic substances (HS) represent the major reservoir of carbon (C) in ecosystems, and their turnover is crucial for understanding the global C cycle. Although basidiomycetes clearly have a role in HS degradation, much less is known about the effect of HS on fungal traits. We studied the alteration of physiological, biochemical, and morphological characteristics of Trametes maxima in the presence of HS. Both complete medium and minimal (C-limited) medium mimicking natural environmental conditions were used. Adding HS led to increased biomass yield, but under C-limited conditions the effect was more apparent. This result indicated that HS were used as an additional substrate and agreed with data showing a greater penetration of tritium-labeled HS into the cell interior under C-limited conditions. Humic substances induced ultra-structural changes in fungal cells, especially under C limitation, including reducing the thicknesses of the hyphal sheath and cell wall. In the minimal medium, cellular respiration increased nearly three-fold under HS application, while the corresponding effect in complete medium was lower. In addition, in the presence of inhibitors, HS stimulated either the cytochrome or the alternative pathway of respiration, depending on presence or absence of glucose in the medium. Our results suggest that, under conditions mimicking the natural environment, HS may play three major roles: as a surplus substrate for fungal growth, as a factor positively affecting cell morphology, and as an activator of physiological respiration.

Keywords

White rot fungus Humic substances as carbon source Cellular respiration Ultra-structural parameters 

Notes

Acknowledgments

The study was supported by The Ministry of Education and Science of the Russian Federation, project 8111.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Olga I. Klein
    • 1
  • Elena P. Isakova
    • 1
  • Yulia I. Deryabina
    • 1
  • Natalia A. Kulikova
    • 1
    • 2
  • Gennadii A. Badun
    • 2
  • Maria G. Chernysheva
    • 2
  • Elena V. Stepanova
    • 1
  • Olga V. Koroleva
    • 1
  1. 1.A.N. Bach Institute of BiochemistryRussian Academy of SciencesMoscowRussia
  2. 2.M.V. Lomonosov Moscow State UniversityMoscowRussia

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