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Journal of Chemical Ecology

, Volume 40, Issue 2, pp 159–168 | Cite as

High Chemical Diversity in a Wasp Pheromone: a Blend of Methyl 6-Methylsalicylate, Fatty Alcohol Acetates and Cuticular Hydrocarbons Releases Courtship Behavior in the Drosophila Parasitoid Asobara tabida

  • Johannes Stökl
  • Anna-Teresa Dandekar
  • Joachim Ruther
Article

Abstract

Wasps of genus Asobara, a larval parasitoid of Drosophila, have become model organisms for the study of host-parasite interactions. However, little is known about the role of pheromones in locating mates and courtship behavior in this genus. In the present study, we aimed to identify the female courtship pheromone in Asobara tabida. The chemical compositions of solvent extracts from male and female wasps were analyzed by GC/MS. These extracts, fractions thereof, and synthetic pheromone candidates were tested for their activity in behavioral bioassays. The results demonstrate that the courtship pheromone of A. tabida is characterized by a remarkable chemical diversity. A multi-component blend of female-specific compounds including methyl 6-methylsalicylate (M6M), fatty alcohol acetates (FAAs), and cuticular hydrocarbons (CHCs) released male courtship behavior. Using a combinatory approach that included both purified natural products and synthetic analogs, it was shown that none of the three chemical classes alone was sufficient to release a full behavioral response in males. However, a blend of M6M and FAAs or combinations of one or both of these with female-derived CHCs resulted in wing-fanning responses by males comparable to those elicited by the crude extract of females. Thus, components from all three chemical classes contribute to the bioactivity of the pheromone, but none of the elements plays a key role or is irreplaceable. The fact that one of the FAAs, vaccenyl acetate, is also used as a kairomone by Asobara females to locate Drosophila hosts suggests that a pre-existing sensory responsiveness to vaccenyl acetate might have been involved in the evolution of the female sex pheromone in Asobara.

Keywords

Asobara tabida Parasitoid wasp Sex pheromone Methyl 6-methylsalicylate Fatty alcohol acetate Cuticular hydrocarbons Sensory exploitation 

Notes

Acknowledgments

We thank Thomas Hoffmeister, University of Bremen, for sending us a starter culture of A. tabida, Michael Brummer for rearing the insects, and Tomer Czaczkes for comments on an earlier version of the manuscript. This study was funded by the German Research Council (Deutsche Forschungsgemeinschaft, DFG; grant STO 966/1-1 to J.S.).

Supplementary material

10886_2014_378_MOESM1_ESM.pdf (139 kb)
ESM 1 (PDF 138 kb)

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Johannes Stökl
    • 1
  • Anna-Teresa Dandekar
    • 1
  • Joachim Ruther
    • 1
  1. 1.Institute of ZoologyUniversity of RegensburgRegensburgGermany

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