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Journal of Chemical Ecology

, Volume 39, Issue 10, pp 1263–1272 | Cite as

A Tetraene Aldehyde as the Major Sex Pheromone Component of the Promethea Moth (Callosamia promethea (Drury))

  • Rafael Gago
  • Jeremy D. Allison
  • J. Steven McElfresh
  • Kenneth F. Haynes
  • Jessica McKenney
  • Angel Guerrero
  • Jocelyn G. MillarEmail author
Article

Abstract

The promethea moth Callosamia promethea is one of three species of silkmoths from the genus Callosamia that occur in North America. Cross attraction of males to heterospecific calling females has been observed in the field, and hybrid progeny have been produced by pairing heterospecifics in captivity. These observations suggest that all three species share or have considerable overlap in the sex attractant pheromones produced by females, so that other prezygotic isolating mechanisms, such as diel differences in reproductive activity, limit hybridization in the field. Coupled gas chromatography-electroantennogram detection and gas chromatography- mass-spectrometry analyses of extracts of volatiles collected from female promethea moths supported the identification of (4E,6E,11Z,13Z)-hexadeca-4,6,11,13-tetraenal [(4E,6E,11Z,13Z)-16:Ald] as the compound in extracts that elicited the largest responses from antennae of males. The identification was confirmed by non-selective synthesis of several isomers as analytical standards, and stereoselective synthesis of (4E,6E,11Z,13Z)-16:Ald for testing in field trials. Male moths were strongly attracted to synthetic (4E,6E,11Z,13Z)-16:Ald, suggesting that this compound is the major and possibly the only component of the sex pheromone of these large saturniid moths. Based on the cross-attraction of heterospecifics, it is likely that this is also a major pheromone component of the other two North American Callosamia species as well.

Keywords

Callosamia promethea Saturniidae (4E,6E,11Z,13Z)-hexadeca-4,6,11,13-tetraenal Sex pheromone 

Notes

Acknowledgments

We are indebted to the Ministry of Science and Innovation for a FPI fellowship to RG. We also thank Shelby Stamper for assistance with the field trials at the UK Arboretum and North Farm. Financial assistance was provided to JDA by the LSU AgCenter, to KFH by Kentucky Agricultural Experiment Station, to AG by CICYT through project AGL2009-13452-C02-01, and to JGM through Hatch project CA-R*-5181-H.

Supplementary material

10886_2013_349_MOESM1_ESM.doc (336 kb)
ESM 1 (DOC 336 kb)
ESM2

Close range response of the Promethea moth, Callosamia promethea, to 100 micrograms of E4,E6,Z11,Z13-16:Ald on a red rubber septum, May 2013, Lexington, Kentucky, USA. (M4V 6.46 mb)

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Rafael Gago
    • 1
  • Jeremy D. Allison
    • 2
    • 3
  • J. Steven McElfresh
    • 4
  • Kenneth F. Haynes
    • 5
  • Jessica McKenney
    • 2
  • Angel Guerrero
    • 1
  • Jocelyn G. Millar
    • 4
    Email author
  1. 1.Department of Biological Chemistry and Molecular ModelingIQAC (CSIC)BarcelonaSpain
  2. 2.Department of EntomologyLouisiana State University Agricultural CenterBaton RougeUSA
  3. 3.Great Lakes Forestry Centre, Natural Resources CanadaSault Ste. MarieCanada
  4. 4.Department of EntomologyUniversity of CaliforniaRiversideUSA
  5. 5.Department of EntomologyUniversity of KentuckyLexingtonUSA

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