Journal of Chemical Ecology

, Volume 39, Issue 3, pp 364–376 | Cite as

Pheromonal Divergence Between Two Strains of Spodoptera frugiperda

  • Melanie Unbehend
  • Sabine Hänniger
  • Robert L. Meagher
  • David G. Heckel
  • Astrid T. Groot
Article

Abstract

Spodoptera frugiperda consists of two genetically and behaviorally different strains, the corn- and the rice-strain, which seem to be in the process of sympatric speciation. We investigated the role of strain-specific sexual communication as a prezygotic mating barrier between both strains by analyzing strain-specific variation in female pheromone composition of laboratory and field strains, and also male attraction in wind tunnel and field experiments. Laboratory-reared and field-collected females from Florida exhibited strain-specific differences in their relative amount of (Z)-7-dodecenyl acetate (Z7-12:OAc) and (Z)-9-dodecenyl acetate (Z9-12:OAc). In wind tunnel assays, we did not find strain-specific attraction of males to females. However, in field experiments in Florida, we observed some differential attraction to synthetic pheromone blends. In a corn field, the corn-strain blend attracted more males of both strains than the rice-strain blend, but both blends were equally attractive in a grass field. Thus, habitat-specific volatiles seemed to influence male attraction to pheromones. In dose–response experiments, corn-strain males were more attracted to 2 % Z7-12:OAc than other doses tested, while rice-strain males were attracted to a broader range of Z7-12:OAc (2–10 %). The attraction of corn-strain males to the lowest dose of Z7-12:OAc corresponds to the production of this compound by females; corn-strain females produced significantly smaller amounts of Z7-12:OAc than rice-strain females. Although corn-strain individuals are more restricted in their production of and response to pheromones than rice-strain individuals, it seems that differences in sexual communication between corn- and rice-strain individuals are not strong enough to cause assortative mating.

Keywords

Sexual communication Male attraction Fall armyworm Corn- and rice-strain Synthetic pheromone lures Dose–response experiments Lepidoptera Noctuidae Sympatric speciation 

Notes

Acknowledgments

This research was funded by the Deutsche Forschungsgemeinschaft (P.S.GR362721) and the Max Planck Gesellschaft. We thank Prof. Manfred Ayasse from the Institute of Experimental Ecology at the University of Ulm in Germany for his help with the wind tunnel experiments. Special thanks to Gregg Nuessly from the Everglades Research and Education Center in Belle Glade and to Tommy Toms, Manager of the Graham Farms in Moore Haven, which supported the male trapping experiments in Florida. We thank Antje Schmaltz and Katja Müller (MPICE, Jena, GER) for their assistance in strain-typing trapped males, and Amy Rowley (USDA, Gainesville, FL) for assistance in the field experiments.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Melanie Unbehend
    • 1
  • Sabine Hänniger
    • 1
  • Robert L. Meagher
    • 2
  • David G. Heckel
    • 1
  • Astrid T. Groot
    • 1
    • 3
  1. 1.Department of EntomologyMPICEJenaGermany
  2. 2.USDA-ARSGainesvilleUSA
  3. 3.Institute for Biodiversity and Ecosystem DynamicsUniversity of AmsterdamAmsterdamthe Netherlands

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