Journal of Chemical Ecology

, Volume 39, Issue 1, pp 67–75 | Cite as

Wax Lipids Signal Nest Identity in Bumblebee Colonies

Article

Abstract

The signalling functions of cuticular lipids, particularly cuticular hydrocarbons, have gained considerable attention in social insect communication. Information transfer between individuals by means of these substances has been examined extensively. However, communication with cuticular lipids is not limited to inter-individual recognition. Cuticular compounds can also have a signalling function in the nest environment. Workers of the bumblebee Bombus terrestris leave cuticular lipid traces, so-called footprints, that mark their nest entrance. In addition, there is evidence that bumblebees sense nesting material to identify their colony. In this study, we examined the signalling potential of bumblebee wax, and tested if bumblebee workers are able to identify their colony with the help of wax scent. Chemical analyses of wax extracts using coupled gas chromatography–mass spectrometry showed that wax from colonies of the bumblebee B. terrestris contained a complex blend of cuticular lipids, dominated by hydrocarbons and wax esters. Comparing the relative compound amounts of wax samples from different colonies, we found that wax scent patterns varied with nest identity. Olfactometer bioassays showed that bumblebees were able to discriminate between wax scents from their own and a foreign colony. Our findings suggest that wax emits characteristic olfactory profiles that are used by workers to recognize their colony.

Keywords

Bombus terrestris Chemical signalling Cuticular hydrocarbons Esters Nest recognition Wax scent 

Notes

Acknowledgments

This study was funded by the German Research Foundation (AY 12/3-1) and the PhD program of the Carl Zeiss Foundation.

Supplementary material

10886_2012_229_MOESM1_ESM.pdf (148 kb)
ESM 1(PDF 147 kb)

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Ann-Marie Rottler
    • 1
  • Stefan Schulz
    • 2
  • Manfred Ayasse
    • 1
  1. 1.Institute of Experimental EcologyUniversity of UlmUlmGermany
  2. 2.Institut für Organische ChemieTechnische Universität BraunschweigBraunschweigGermany

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