New Sex Pheromone Blend for the Lightbrown Apple Moth, Epiphyas postvittana

  • Ashraf M. El-Sayed
  • Vanessa J. Mitchell
  • Lee-Anne M. Manning
  • David Max Suckling
Article

Abstract

The composition of the sex pheromone gland of the lightbrown apple moth, Epiphyas postvittana (Walker) was re-investigated. In addition to the two previously identified compounds, (E)-11-tetradecenyl acetate (E11-14Ac) and (E,E)-9,11-tetradecadienyl acetate (E9E11-14Ac), seven additional candidate pheromone compounds were identified: (E)-11-tetradecen-1-ol (E11-14OH), tetradecyl acetate, hexadecanal, (E)-11-hexadecenyl acetate (E11-16Ac), hexadecyl acetate, octadecanal, and octadecyl acetate. Gas chromatographic-electroantennographic detection analysis showed that only four (of the nine) compounds (E11-14OH, E11-14Ac, E9E11-14Ac, and E11-16Ac) elicited electrophysiological responses. When either E11-14OH or E11-16Ac were tested at various ratios in three-component blends with both E11-14Ac and E9E11-14Ac, no increase in trap catch was observed compared to that to the previously identified binary blend. However, when these two compounds (1% E11-14OH and 0.5% E11-16Ac) were both added to E11-14Ac and E9E11-14Ac, trap catch was roughly double that to the previously identified binary blend alone. The new four-component blend should be more sensitive for detecting this economically important insect, especially in low population areas (i.e., in newly invaded habitats). In addition, this new blend may enhance pheromone control of this pest, through approaches such as mating disruption, lure and kill, and mass trapping.

Key Words

Lightbrown apple moth Epiphyas postvittana Sex pheromone Pheromone identification Monitoring and control Lepidoptera Tortricidae 

References

  1. Bailey, P., Baker, G., and Caon, G. 1996. Field efficacy and persistence of Bacillus thuringiensis var. kurstaki against Epiphyas postvittana (Walker) (Lepidoptera:Tortricidae) in relation to larval behavior on grapevine leaves. Aust. J. Entomol. 35:297–302.CrossRefGoogle Scholar
  2. Baker, T. C., Meyer, W., and Roelofs, W. L. 1981. Sex pheromone dosage and blend specificity of response by oriental fruit moth females. Entomol. Exp. Appl. 30:269–279.CrossRefGoogle Scholar
  3. Bellas, T. E., Bartell, R. J. and Hill, A. 1983 Identification of two components of the sex pheromone of the moth, Epiphyas postvittana (Lepidoptera, Tortricidae). J. Chem. Ecol. 9:503–511.CrossRefGoogle Scholar
  4. El-Sayed, A., Godde, J., Witzgall, P., and Arn, H. 1999. Characterisation of pheromone blend for grapevine moth, Lobesia botrana by using flight track recording. J. Chem. Ecol. 25:389–400.CrossRefGoogle Scholar
  5. El-Sayed, A. M., Suckling, D. M., Wearing, C. H., and Byers, J. A. 2006. Potential of mass trapping for long-term pest management and eradication of invasive species. J. Econ. Entomol. 99:1550–1564.PubMedCrossRefGoogle Scholar
  6. El-Sayed, A. M., Suckling, D. M., Byers, J. A, Jang E. B., and Wearing, C. H. 2009. Potential of “lure and kill” for long-term pest management and eradication of invasive species. J. Econ. Entomol. 102:815–835.PubMedCrossRefGoogle Scholar
  7. El-Sayed, A. M. 2011. The Pherobase: Database of Insect Pheromones and Semiochemicals. http://www.pherobase.com.
  8. Foster, S. P., and Roelofs, W. L. 1990. Biosynthesis of a monoene and a conjugated diene sex pheromone component of the lightbrown apple moth by D11 desaturation. Experientia. 46:269–273.CrossRefGoogle Scholar
  9. Gray, T. G., Slessor, K. N., Grant, G. G., Shepherd, R. F., Holsten, E. H., and Tracey, A. S. 1984. Identification and field testing of pheromone components of Choristoneura orae (Lepidoptera: Tortricidae). Can. Entomol. 116:51–56.CrossRefGoogle Scholar
  10. Guerin, P. M., Arn, H., Buser, H. R., and Charmillot, P. J. 1986. Sex pheromones of Adoxophyes orana: Additional components and variability in ratio of (Z)-9- and (Z)-11-tetradecenyl acetate. J. Chem. Ecol. 12:763–771.CrossRefGoogle Scholar
  11. Hitchcock, B. 2009. Protecting Australia’s exports: revision of the genus Epiphyas. CRCNPB 2009 Science Exchange meeting 22–24 September 2009, Sunshine Coast, Queensland, Australia.Google Scholar
  12. Horak, M., Whittle, C. P., Bellas, T. E., and Rumbo, E. R. 1988. Pheromone gland components of some Australian tortricids in relation to their taxonomy. J. Chem. Ecol. 14:1163–1175.CrossRefGoogle Scholar
  13. Linn, C. E., and Roelofs, W. L. 1983.. Effect of varying proportions of the alcohol component on sex pheromone blend discrimination in male oriental fruit moths. Physiol. Entomol. 8:291–306.CrossRefGoogle Scholar
  14. Lo, P. L., Suckling, D. M., Walker, J. T. S., Shaw, P. W., and Burnip, G. M. 2000. Feeding site preferences of leafrollers (Lepidoptera: Tortricidae) on apple trees. N. Z. J. Crop Hortic. Sci. 28:235–43.CrossRefGoogle Scholar
  15. Muggleston, S. J., and Foster, S. P. 1989. Sustained-flight tunnel response of male lightbrown apple moth to synthetic sex pheromone. Physiol. Entomol. 14:443–449.CrossRefGoogle Scholar
  16. Neal, J. W., Jr., Klun, J. A., Bierl-Leonhardt, B. A., and Schwarz, M. 1982. Female sex pheromone of Choristoneura parallela (Lepidoptera: Tortricidae). Environ. Entomol. 11:893–896.Google Scholar
  17. Sas Institute Inc. 1998. Statview. Cary, North Carolina: SAS Institute Inc.Google Scholar
  18. Scott, P. W. 1995. Techniques and Practice of Chromatography. Marcel Dekker, New York.Google Scholar
  19. Suckling, D. M., and Shaw, P. W. 1992. Conditions that favor mating disruption of Epiphyas postvittana (Lepidoptera: Tortricidae). Env. Ent. 21:949–956.Google Scholar
  20. Suckling, D. M. and Brockerhoff, E. G. 1999. Control of lightbrown apple moth, Epiphyas postvittana (Lepidoptera: Tortricidae) using an attracticide. J. Econ. Entomol. 92:367–372.Google Scholar
  21. Suckling, D. M. and Brockerhoff, E. G. 2010. Invasion biology, ecology, and management of the light brown apple moth (Tortricidae). Annu. Rev. Entomol. 55:285–306.PubMedCrossRefGoogle Scholar
  22. Wearing, C. H., Thomas, W. P., Dugdale, J. S., and Danthanarayana, W. 1991. Tortricid pests of pome and stone fruits, Australian and New Zealand species. pp. 453–72, in L. van der Geest, H. H. Evenhuis (eds.). Tortricid Pests: Their Biology, Natural Enemies and Control. Elsevier, Amsterdam.Google Scholar
  23. Witzgall, P. 1990. Attraction of Cacoecimorpha pronubana male moths to synthetic sex pheromone blends in the wind tunnel. J. Chem. Ecol. 16:1507–1516.CrossRefGoogle Scholar
  24. Witzgall, P., Bengtsson, M., Buser, H. R., Chambon, P. J., Priesner, E., Wildbolz, T., and Arn, H. 1991. Sex pheromones of Spilonota ocellana and Spilonota laricana. Entomol. Exp. Appl. 60:219–223.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Ashraf M. El-Sayed
    • 1
  • Vanessa J. Mitchell
    • 1
  • Lee-Anne M. Manning
    • 1
  • David Max Suckling
    • 1
  1. 1.The New Zealand Institute for Plant & Food Research LimitedLincolnNew Zealand

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