Journal of Chemical Ecology

, 37:1013

Effect of Water Stress and Fungal Inoculation on Monoterpene Emission from an Historical and a New Pine Host of the Mountain Pine Beetle

  • Inka Lusebrink
  • Maya L. Evenden
  • F. Guillaume Blanchet
  • Janice E. K. Cooke
  • Nadir Erbilgin
Article

DOI: 10.1007/s10886-011-0008-3

Cite this article as:
Lusebrink, I., Evenden, M.L., Blanchet, F.G. et al. J Chem Ecol (2011) 37: 1013. doi:10.1007/s10886-011-0008-3

Abstract

The mountain pine beetle (Dendroctonus ponderosae, MPB) has killed millions of lodgepole pine (Pinus contorta) trees in Western Canada, and recent range expansion has resulted in attack of jack pine (Pinus banksiana) in Alberta. Establishment of MPB in the Boreal forest will require use of jack pine under a suite of environmental conditions different from those it typically encounters in its native range. Lodgepole and jack pine seedlings were grown under controlled environment conditions and subjected to either water deficit or well watered conditions and inoculated with Grosmannia clavigera, a MPB fungal associate. Soil water content, photosynthesis, stomatal conductance, and emission of volatile organic compounds (VOCs) were monitored over the duration of the six-week study. Monoterpene content of bark and needle tissue was measured at the end of the experiment. β-Phellandrene, the major monoterpene in lodgepole pine, was almost completely lacking in the volatile emission profile of jack pine. The major compound in jack pine was α-pinene. The emission of both compounds was positively correlated with stomatal conductance. 3-Carene was emitted at a high concentration from jack pine seedlings, which is in contrast to monoterpene profiles of jack pine from more southern and eastern parts of its range. Fungal inoculation caused a significant increase in total monoterpene emission in water deficit lodgepole pine seedlings right after its application. By 4 weeks into the experiment, water deficit seedlings of both species released significantly lower levels of total monoterpenes than well watered seedlings. Needle tissue contained lower total monoterpene content than bark. Generally, monoterpene tissue content increased over time independent from any treatment. The results suggest that monoterpenes that play a role in pine-MPB interactions differ between lodgepole and jack pine, and also that they are affected by water availability.

Key Words

Pinus contorta Pinus banksiana VOCs Monoterpenes Tree defense Grosmannia clavigera Mountain pine beetle 

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Inka Lusebrink
    • 1
    • 3
  • Maya L. Evenden
    • 1
  • F. Guillaume Blanchet
    • 2
  • Janice E. K. Cooke
    • 1
  • Nadir Erbilgin
    • 3
  1. 1.Department of Biological SciencesUniversity of AlbertaEdmontonCanada
  2. 2.Department of Renewable ResourcesUniversity of AlbertaEdmontonCanada
  3. 3.Department of Renewable ResourcesUniversity of AlbertaEdmontonCanada

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