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Journal of Chemical Ecology

, Volume 36, Issue 9, pp 1017–1028 | Cite as

Phenolic Composition and Antioxidant Capacity of Bilberry (Vaccinium myrtillus) Leaves in Northern Europe Following Foliar Development and Along Environmental Gradients

  • Françoise MartzEmail author
  • Laura Jaakola
  • Riitta Julkunen-Tiitto
  • Sari Stark
Article

Abstract

Bilberry is a characteristic field layer species in the boreal forests and is an important forage plant for herbivores of the North European ecosystem. Bilberry leaves contain high levels of phenolic compounds, especially hydroxycinnamic acids, flavonols, catechins, and proanthocyanidins. We investigated the phenolic composition of bilberry leaves in two studies, one following foliar development in forest and open areas, and the other along a wide geographical gradient from south to north boreal forests in Finland. An analysis of bilberry leaves collected in open and forest areas showed that major phenolic changes appeared in the first stages of leaf development, but, most importantly, synthesis and accumulation of flavonoids was delayed in the forest compared to the high light sites. Sampling along a geographical gradient in the boreal zone indicated that leaves from higher latitudes and higher altitudes had greater soluble phenolic and flavonol levels, higher antioxidant capacity, and lower contents of chlorogenic acid derivatives. The ecological significance of the results is discussed.

Key Words

Vaccinium myrtillus Bilberry Flavonol Soluble phenolics Flavonoid synthesis DPPH Antioxidant capacity Boreal zone Development Latitude Altitude 

Notes

Acknowledgments

The authors thank K. Mikkola for providing background information for site selection, R. Nielsen and M. Weissman for technical help, and J. Hyvönen for statistical advices. We are indebted to everyone involved in the sampling that was done in connection with the BioSoil project (Finnish Forest Research Institute). This work was supported by the European Commission, Regional Development Fund (project “Mette” number 70025/05), the Lapland Regional Fund of the Finnish Cultural Foundation (to F. M.), the Kone Foundation, Finland (to F. M.), and the Academy of Finland (grant no 09141 to L. J.).

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Françoise Martz
    • 1
    Email author
  • Laura Jaakola
    • 2
  • Riitta Julkunen-Tiitto
    • 3
  • Sari Stark
    • 1
  1. 1.Finnish Forest Research InstituteRovaniemiFinland
  2. 2.Department of BiologyUniversity of OuluOuluFinland
  3. 3.Natural Product Research Laboratories, Faculty of BiosciencesUniversity of JoensuuJoensuuFinland

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