A Metabolomic Approach to Identifying Chemical Mediators of Mammal–Plant Interactions
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Different folivorous marsupials select their food from different subgenera of Eucalyptus, but the choices cannot be explained by known antifeedants, such as formylated phloroglucinol compounds or tannins, or by nutritional quality. Eucalypts contain a wide variety of plant secondary metabolites so it is difficult to use traditional methods to identify the chemicals that determine food selection. Therefore, we used a metabolomic approach in which we employed 1H nuclear magnetic resonance spectroscopy to compare chemical structures of representatives from the two subgenera and to identify chemicals that consistently differ between them. We found that dichloromethane extracts of leaves from most species in the subgenus Eucalyptus differ from those in Symphyomyrtus by the presence of free flavanones, having no substitution in Ring B. Although flavanoids are known to deter feeding by certain insects, their effects on marsupials have not been established and must be tested with controlled feeding studies.
Key WordsMetabolomics Eucalyptus Symphyomyrtus Folivorous marsupials Common brushtail possum 1H NMR spectroscopy Flavanones Herbivory
We wish to thank Cora Shiroyama for help in collecting the samples, Lynnette Lambert for assistance with 1H NMR spectroscopy and Professor Eckhard Wollenweber for valuable discussions and a generous gift of reference samples. Andras Keszei helped with the figures. The comments of three anonymous reviewers led to marked improvements in the manuscript.
- Bick, I. R. C., Brown, R. B., and Hillis, W. E. 1972. Three flavanones from the leaves of Eucalyptus sieberi. Aust. J. Chem. 25:449–451.Google Scholar
- Boland, J., Brophy, J. J., and House, A. P. N. 1991. Eucalyptus Leaf Oils: Use, Chemistry, Distillation and Marketing. Inkata Press, Melbourne.Google Scholar
- Foley, W. J., Mcilwee, A., Lawler, I., Aragones, L., Woolnough, A. P., and Berding, N. 1998. Ecological applications of near infrared reflectance spectroscopy a tool for rapid, cost-effective prediction of the composition of plant and animal tissues and aspects of animal performance. Oecologia 116:293–305.CrossRefGoogle Scholar
- Horn, D. H. S., and Lamberton, J. A. C. 1963. Nuclear magnetic resonance (NMR) study of a new flavonoid. Chem. Ind. (Lond.):691–2.Google Scholar
- Jia, Q., Nichols, T. C., Rhoden, E. E., and Waite, S. 2003. Identification of free-B-ring flavonoids as potent cyclooxygenase 2 (COX-2) inhibitors. U.S. Pat. Appl. Publ., US 2003165588 A1 20030904.Google Scholar
- Moore, B. D., Wallis, I. R., Marsh, K. J., and Foley, W. J. 2004a. The role of nutrition in the conservation of the marsupial folivores of eucalypt forests. pp. 549–575, in D. Lunney (ed.). Conservation of Australia’s Forest Fauna. 2nd ed. Royal Zoological Society of New South Wales, Mosman, NSW, Australia.Google Scholar
- Pryor, L. D. 1959. Species distribution and association in Eucalyptus, pp. 461–471, in A. Keast, R. L. Crocker, and C. S. Christian (eds.). Biogeography and Ecology in Australia. W. Junk, The Hague.Google Scholar
- Scrivener, N. J., Johnson, C. N., Wallis, I. R., Takasaki, M., Foley, W. J., and Krockenberger, A. K. 2004. Which trees do wild common brushtail possums (Trichosurus vulpecula) prefer? Problems and solutions in scaling laboratory findings to diet selection in the field. Evol. Ecol. Res. 6:77–87.Google Scholar
- Wallis, I. R., and Foley, W. J. 2003. Independent validation of near-infrared reflectance spectroscopy as an estimator of potential food intake of Eucalyptus foliage for folivorous marsupials Aust. J. Zool. 51:95–98.Google Scholar
- Wollenweber, E., and Kohorst, G. 1981. Epicuticular leaf flavonoids from Eucalyptus species and from Kalmia latifolia. Z. Naturforsch., C: Biosci. 36:913–915.Google Scholar