Journal of Chemical Ecology

, Volume 36, Issue 6, pp 662–669 | Cite as

High Molecular Size Humic Substances Enhance Phenylpropanoid Metabolism in Maize (Zea mays L.)

  • Michela Schiavon
  • Diego Pizzeghello
  • Adele Muscolo
  • Silvia Vaccaro
  • Ornella Francioso
  • Serenella Nardi
Article

Abstract

A high molecular weight humic fraction (>3,500 Da) was characterized chemically by DRIFT and 1H NMR spectroscopy, and was applied to Zea mays L. plants to evaluate its effect on phenylpropanoid metabolism. The activity and gene expression of phenylalanine (tyrosine) ammonia-lyase (PAL/TAL), and the concentrations of phenolics and their amino acid precursors phenylalanine and tyrosine were assayed. Maximum induction of PAL/TAL activity and expression was obtained when the concentration of added humic substance was 1 mg C/l hydroponic solution. Phenylalanine and tyrosine significantly decreased (−16% and −22%, respectively), and phenolic compounds increased in treated plants. The effects of the humic substance could be ascribed partly to indoleacetic acid (27 nmol/mg C) in the humic fraction. Our results suggest that this humic fraction induces changes in phenylpropanoid metabolism. This is the first study that shows a relationship between humic substances and the phenylpropanoid pathway.

Key Words

High molecular weight humic substances Zea mays L. DRIFT (Diffuse Reflectance Infrared Fourier Transform spectroscopy) 1H NMR (Proton Nuclear Magnetic Resonance spectroscopy) Phenylalanine (tyrosine) ammonia-lyase Phenolics 

Notes

Acknowledgements

This work was partially supported by a grant from the University of Padova (ex 60%, 2008) awarded to Prof. Serenella Nardi. We are grateful to Dr. Andrea Ertani and Dr. Sara Trevisan for their helpful assistance. The authors would like to thank Professor Ann E. Hagerman for her precious help in the improvement of the manuscript.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Michela Schiavon
    • 1
  • Diego Pizzeghello
    • 1
  • Adele Muscolo
    • 2
  • Silvia Vaccaro
    • 1
  • Ornella Francioso
    • 3
  • Serenella Nardi
    • 1
  1. 1.Department of Agricultural BiotechnologiesUniversity of PadovaLegnaro, PadovaItaly
  2. 2.Department of Agriculture and Forest Systems ManagementMediterranean University of Reggio CalabriaReggio di CalabriaItaly
  3. 3.Department of Agroenvironmental Science and TechnologyUniversity of BolognaBolognaItaly

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