Journal of Chemical Ecology

, Volume 36, Issue 2, pp 192–199 | Cite as

Herbivore- and Elicitor-Induced Resistance in Rice to the Rice Water Weevil (Lissorhoptrus oryzophilus Kuschel) in the Laboratory and Field

  • Jason C. Hamm
  • Michael J. Stout
  • Rita M. Riggio


Feeding by herbivores can change plants in ways that make them more resistant to subsequent herbivory. Such induced responses are better-studied in a number of model dicots than in rice and other cereals. In a series of greenhouse and field experiments, we assessed the effects of prior herbivory by the fall armyworm, Spodoptera frugiperda (J.E. Smith) and of exogenous applications of jasmonic acid (JA) on the resistance of rice plants to the rice water weevil, Lissorhoptrus oryzophilus (Kuschel), the major pest of rice in the United States. Prior feeding by S. frugiperda and treatment of plants with exogenous JA resulted in increases in the resistance of plants to the weevil. Increases in resistance were manifested as reduced numbers of eggs and first-instars associated with armyworm-injured or JA-treated plants relative to control plants. In field experiments, there was a transient but significant reduction in the number of immature L. oryzophilus on JA-treated plants relative to untreated plants. To our knowledge, this is the first example of direct induced resistance in rice demonstrated in small-plot field experiments. We discuss the potential for the use of elicitor induced resistance in rice.


Jasmonic acid Induced resistance Elicitors Oryza sativa Lissorhoptrus oryzophilus Spodoptera frugiperda 



The authors thank Marty Frey for assistance with field work and Drs. Gregg Henderson, Fangneng Huang, Natalie Hummel, Gene Reagan, and Richard Story for valuable comments and suggestions on the manuscript.


  1. Bentur, J. S. and Kalode, M. B. 1996. Hypersensitive reaction and induced resistance in rice against the Asian rice gall midge Orseolia oryzae. Ent. Exe. et Applic. 78:77–81.CrossRefGoogle Scholar
  2. Black, C. A., Karban, R., Godfrey, L., Granett, J., and Chaney, W. E. 2003. Jasmonic acid: A vaccine against leafminers (Diptera: Agromyzidae) in celery. Environ. Entomol. 32:1196–1202.Google Scholar
  3. Browse, J. and Howe, G. 2008. New weapons and a rapid response against insect attack. Plant Physiol. 146:832–838.CrossRefPubMedGoogle Scholar
  4. Bruinsma, M. and Dicke, M. 2008. Herbivore-induced indirect defense: from induction mechanisms to community ecology. In Herbivore-Induced Indirect Defense: From Induction Mechanisms to Community Ecology, A. Schaller, ed., Springer Publishing, The Netherlands, 31–60.Google Scholar
  5. Dicke, M., Van Poecke, R. M. P., and De Boer, J. G. 2003. Inducible indirect defence of plants: from mechanisms to ecological functions. Basic Appl. Ecol. 4:27–42.CrossRefGoogle Scholar
  6. Everett, T. R. and Trahan, G. B. 1967. Oviposition by rice water weevils in Louisiana. J. Econ. Entomol. 60:305–307.Google Scholar
  7. Farmer, E. E. and Ryan, C. A. 1990. Interplant communication: airborne methyl jasmonate induces synthesis of proteinase inhibitors in plant leaves. Proc. Natl. Acad. Sci. USA 87:7713–7716.CrossRefPubMedGoogle Scholar
  8. Heinrichs, E. A., Medrano, F. G., and Rapusas, H. R. 1985. Genetic evaluation for insect resistance in rice. International Rice Research Institute, Los Baños.Google Scholar
  9. Inbar, M., Doostdar, H., Sonoda, R. M., Leibee, G. L., and Mayer, R. T. 1988. Elicitors of plant defense systems reduce insect densities and disease incidence. J. Chem. Ecol. 24:135–149.CrossRefGoogle Scholar
  10. Kanno, H, Satoh, M., Kimura, T., and Fujita, Y. 2005. Some aspects of induced resistance to rice blast fungus, Magnaporthe grisea, in rice plant infested by white-backed planthopper, Sogatella furcifera. Appl. Entomol. Zool. 40:91–97.CrossRefGoogle Scholar
  11. Karban, R. and Baldwin, I. T. 1997. Induced responses to herbivory. University of Chicago Press, Chicago.Google Scholar
  12. Karban, R. and Chen, Y. 2007. Induced resistance in rice against insects. Bull. Entomol. Res. 97:327–335.CrossRefPubMedGoogle Scholar
  13. Kessler, A. and Baldwin, I. T. 2002. Plant responses to insect herbivory: the emerging molecular analysis. Annu. Rev. Plant Bio. 53:299–328.CrossRefGoogle Scholar
  14. Kogel, K. H. and Langen, G. 2005. Induced disease resistance and gene expression in cereals. Cellular Microbiol. 7:1555–1564.CrossRefGoogle Scholar
  15. Korth, K. and Thompson, G. A. 2006. Chemical signals in plants: jasmonates and the role of insect-derived elicitors in responses to herbivores. In Multigenic and Induced Systemic Resistance in Plants, S. Tuzun and E. Bent, eds., Springer Publishing, New York, 259–278.Google Scholar
  16. Lou, Y., Du, M., Turlings, T. C. J., Cheng, J., and Shan, W. 2005. Exogenous applications of jasmonic acid induces volatile emissions in rice and enhances parasitism of Nilaparvata lugens eggs by the parasitoid Anagrus nilaparvatae. J. Chem. Ecol. 31:1985–2002.CrossRefPubMedGoogle Scholar
  17. Matsumura, M. and Suzuki, Y. 2003. Direct and feeding-induced interactions between two rice planthoppers, Sogatella furcifera and Nilaparvata lugens: effects on dispersal capability and performance. Ecol. Entomol. 28:174–182.CrossRefGoogle Scholar
  18. Mei, C., Qi, M., Sheng, G., and Yang, Y. 2006. Inducible overexpression of a rice allene oxide synthase gene increases the endogenous jasmonic acid level, PR gene expression and host resistance to fungal infection. Mol. Plant-Microb. Interact. 19:1127–1137.Google Scholar
  19. N’guessan, F. K., Quisenberry, S. S., Thompson, R. A., and Linscombe, S. D. 1994. Assessment of Louisiana rice breeding lines for tolerance to the rice water weevil (Coleoptera: Curculionidae). J. Econ. Entomol. 87:476–481.Google Scholar
  20. Omer, A. D., Thaler, J. S., Granett, J., and Karban, R. 2000. Jasmonic acid induced resistance in grapevines to a root and leaf feeder. J. Econ. Entomol. 93(3):840–845.Google Scholar
  21. Omer, A. D., Granett, R., Karban, R., and Villa, M. 2001. Chemically-induced resistance against multiple pests in cotton. Intl. J. Pest Manage. 47(1):49–54.CrossRefGoogle Scholar
  22. SAS Institute. 2007. SAS v. 9.1. SAS Institute, Cary, NC.Google Scholar
  23. Satoh, M., Nakajima, T., and Kanno, H. 2005. Induced resistance to rice blast disease in rice plants infested with the whitebacked planthopper, Sogatella furcifera, in a paddy field. Japanese Journal of App. Entomol. Zool. 49:105–111.CrossRefGoogle Scholar
  24. Seino, Y., Suzuki, Y., and Sogawa, K. 1996. An ovicidal substance produced by rice plants in response to oviposition by the whitebacked planthopper, Sogatella furcifera (Horvath) (Homoptera: Delphacidae). App. Entomol. Zool. 31:467–473.Google Scholar
  25. Senthil-Nathan, S., Choi, M. Y., Paik, C. H., Seo, H. Y., and Kalaivani, K. 2009. Toxicity and physiological effects of neem pesticides applied to rice on the Nilaparvata lugens Stål, the brown planthopper. Ecotoxicol. Environ. Safety 72:1707–1713.CrossRefPubMedGoogle Scholar
  26. Smith, C. M. 1983. The rice water weevil, Lissorhoptrus oryzophilus Kuschel, pp. 3–8, in K. G. Singh (ed.). Exotic Plant Quarantine Pests and Procedures for Introduction of Plant Materials. ASEAN (PLANTI), Selangor.Google Scholar
  27. Stout, M. J. and Bostock, R. M. 1999. Specificity of induced responses to arthropods and pathogens, pp. 117–136, in A. A. Agrawal, S. Tuzun, and E. Bent (eds.). Induced Plant Defenses Against Pathogens and Herbivores. The American Phytopathological Society Press, St. Paul, MN.Google Scholar
  28. Stout, M. J. and Riggio, M. R. 2002. Variation in susceptibility of rice lines to infestation by the rice water weevil (Coleoptera: Curculionidae). Journal Agric. Urban Entomol. 19:205–216.Google Scholar
  29. Stout, M. J., Rice, W. C., Riggio, R. N., and Ring, D. R. 2000. The effects of four insecticides on the population dynamics of the rice water weevil Lissorhoptrus oryzophilus. J. Entomol. Sci. 35:48–61.Google Scholar
  30. Stout, M. J., Riggio, M. R., Zou, L., and Roberts, R. 2002. Flooding influences ovipositional and feeding behavior of the rice water weevil (Coleoptera: Curculionidae). J. Econ. Entomol. 95:715–721.CrossRefGoogle Scholar
  31. Stout, M. J., Riggio, M. R., and Yang, Y. 2009. Direct Induced Resistance in Oryza sativa to Spodoptera frugiperda. Environ. Entomol. 38(4):1174–1181.CrossRefPubMedGoogle Scholar
  32. Thaler, J. S. 1999. Induced resistance in agricultural crops: effects of jasmonic acid on herbivory and yield in tomato plants. Environ. Entomol. 28:30–37.Google Scholar
  33. Thaler, J. S., Stout, M. J., Karban, R., and Duffey, S. S. 1996. Exogenous jasmonates stimulate insect wounding in tomato plants (Lycopersicon esculentum) in the laboratory and field. J. Chem. Ecol. 22:1767–1781.CrossRefGoogle Scholar
  34. Thaler, J. S., Farag, M. A., Pare, P. W., and Dicke, M. 2002. Jasmonate-deficient plants have reduced direct and indirect defenses against herbivores. Ecol. Lett. 5:764–774.CrossRefGoogle Scholar
  35. Walling, L. L. 2000. The myriad plant responses to herbivores. J. Plant Growth Regul. 19:3187–3195.Google Scholar
  36. Wasternack, C. 2007. Jasmonates: An update on biosynthesis, signal transduction and action in plant stress response, growth and development. Ann. Bot. 100:681–697.CrossRefGoogle Scholar
  37. Way, M. O. 1990. Insect pest management in rice in the United States, pp 181–189, in B. T. Grayson, M. B. Green, and L. G. Copping (eds.). Pest Management in Rice. Elsevier Science, New York.Google Scholar
  38. Xu, T., Zhou, Q., Xia, Q., Zhang, W. Q., and Gu, D. X. 2002. Effects of herbivore-induced rice volatiles on the host selection behavior of brown planthopper, Nilaparvata lugens. Chin. Sci. Bull. 47:1355–1360.CrossRefGoogle Scholar
  39. Xu, T., Zhou, Q., Chen, W., Zhang, G., He, G., Gu, D., and Zhang, W. 2003. Involvement of jasmonate-signaling pathway in the herbivore-induced rice plant defense. Chin. Sci. Bull. 48:1982–1987.CrossRefGoogle Scholar
  40. Yuan, J. S., Kollner, T. G., Wiggins, G., Grant, J., Degenhardt, J., and Chen, F. 2008. Molecular and genomic basis of volatile-mediated indirect defense against insects in rice. Plant J. 55:491–503.CrossRefGoogle Scholar
  41. Zhou, Q., Xu, T., Zhang, G. R., Gu, D. X., and Zhang, W. Q. 2003. Repellent effects of herbivore-induced rice volatiles on the brown planthopper, Nilaparvata lugens. Acta Entomologia Sinica 46:739–744.Google Scholar
  42. Zou, L., Stout, M. J., and Dunand, R. T. 2004. The effects of feeding by the rice water weevil, Lissorhoptrus oryzophilus Kuschel, on the growth and yield components of rice, Oryza sativa. Agric. For. Entomol. 6:47–53.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Jason C. Hamm
    • 1
  • Michael J. Stout
    • 1
  • Rita M. Riggio
    • 1
  1. 1.Department of EntomologyLouisiana State University Agricultural CenterBaton RougeUSA

Personalised recommendations