Reduced Photosynthetic Activity is Directly Correlated with 2-(3H)-benzoxazolinone Accumulation in Lettuce Leaves
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2-(3H)-Benzoxazolinone (BOA) is a secondary plant metabolite previously found to inhibit plant growth and development. The phytotoxic activity of BOA has been extensively demonstrated over the last years. However, the relation of BOA phytotoxicity with BOA accumulation in plant leaves has not been thoroughly investigated. In this work, BOA phytotoxicity on photosynthesis (ΦPSII and Pn) of lettuce (Lactuca sativa L. cv. Great Lakes) was studied, and these results were correlated with BOA quantities in the leaves. BOA-treated plants showed reduced photosynthesis rate 6 h after the beginning of the treatment, and the efficiency of photosystem II started to be affected 10 h after treatment. These results were correlated with an increasing concentration of BOA in leaves that starts 6 h after treatment and shows a maximum at 96 h.
KeywordsBOA action HPLC Photosynthetic activity Phytotoxicity
The authors are grateful to Oliver Weiss for help in the photosynthesis measurements, and Eva Diéguez and Francisco Macías from the University of Cádiz for their help with the HPLC measurements and their wise advice.
- BAERSON, S., SÁNCHEZ-MOREIRAS, A. M., PEDROL, N., SCHULZ, M., KAGAN, I. A., AGARWAL, A. K., REIGOSA, M. J., and DUKE, S. O. 2005. Detoxification and transcriptome response in Arabidopsis seedlings exposed to the allelochemical benzoxazolin-2(3H)-one. J. Biol. Chem. 280:21867–21881.CrossRefPubMedGoogle Scholar
- FRIEBE, A., VILICH, V., HENNING, L., KLUGE, M., and SICKER, D. 1998. Detoxification of benzoxazolinone allelochemicals from wheat by Gaeumannonmyces graminis var. tritici, G. graminis var. graminis, G. graminis var. avenae and Fusarium culmorum. Appl. Envir. Microbiol. 64:2386–2391. Google Scholar
- HALL, D., and RAO, K. 1999. Photosynthesis: Studies in Biology. Cambridge University Press, UK.Google Scholar
- MACÍAS, F. A., MATEOS, D. M., OLIVEROS-BASTIDAS, A., CASTELLANO, D., SIMONET, A. M., and GONZÁLEZ-MOLINILLO, J. M. 2006. Structure-activity relationship (SAR) studies of benzoxazinones, their degradation products, and analogues. Phytotoxicity on problematic weeds Avena fatua l. and Lolium rigidum Gaud. J. Agric. Food Chem. 54:1040–1048.Google Scholar
- SCHULZ, M., KNOP, M., KANT, S., SICKER, D., VOLOSHCHUK, N., and GRYGANSKI, A. 2006. Detoxification of allelochemicals—the case of benzoxazolin-2(3H)-one (BOA). pp. 157–170 in M. J. REIGOSA, N. PEDROL and L. GONZÁLEZ (eds.). Allelopathy: A Physiological Process with Ecological Implications. Springer, Dordrecht, The Netherlands. Google Scholar