Journal of Chemical Ecology

, Volume 35, Issue 6, pp 689–697 | Cite as

Two Regulatory Mechanisms of Monoterpenoid Pheromone Production in Ips spp. Of Bark Beetles

  • Jeremy C. Bearfield
  • Anastasia G. Henry
  • Claus Tittiger
  • Gary J. Blomquist
  • Matthew D. Ginzel
Article

Abstract

Bark beetles use aggregation pheromones to coordinate host colonization and mating. These monoterpenoid chemical signals are produced de novo in midgut cells via the mevalonate pathway, and pheromone production is induced when an adult beetle feeds on phloem of a host tree. In Ips pini, juvenile hormone (JH) III influences key regulatory enzymes along the mevalonate pathway that leads to pheromone production. In fact, topically applied JH III is sufficient to stimulate pheromone production in unfed males. In this study, we explore the influence of feeding and JH III treatment on pheromone production in male Ips confusus, the pinyon Ips. We also characterize the influence of feeding and JH III treatment on transcript levels and activity of three key enzymes involved in pheromone biosynthesis: 3-hydroxy-3-methylglutaryl-CoA (HMG) synthase (HMGS), HMG-CoA reductase (HMGR) and geranyl diphosphate synthase (GPPS). We also extend the current understanding of the regulation of pheromone biosynthesis in I. pini, by measuring the influence of feeding and JHIII treatment on enzymatic activity of HMGS and GPPS. Feeding on host phloem alone strongly induces pheromone production in male I. confusus, while JH III treatment has no effect. However, feeding and JH III both significantly up-regulate mRNA levels of key mevalonate pathway genes. Feeding up-regulates these genes to a maximum at 32 h, whereas with JH III-treatment, they are up-regulated at 4, 8, and 16 h, but return near to non-treatment levels at 32 h. Feeding, but not JH III treatment, also increases the activity of all three enzymes in I. confusus, while both feeding or treatment with JH III increase HMGS and GPPS activity in I. pini. Our data suggest that pheromone production in Ips is not uniformly controlled by JH III and feeding may stimulate the release of some other regulatory factor, perhaps a brain hormone, required for pheromone production.

Keywords

Ips confusus Ips pini Pheromones Juvenile hormone Mevalonate pathway 

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Jeremy C. Bearfield
    • 1
  • Anastasia G. Henry
    • 2
  • Claus Tittiger
    • 1
  • Gary J. Blomquist
    • 1
  • Matthew D. Ginzel
    • 3
  1. 1.Department of Biochemistry and Molecular BiologyUniversity of Nevada, RenoRenoUSA
  2. 2.Tetrad Program, Department of Cell BiologyUniversity of California, San FranciscoSan FranciscoUSA
  3. 3.Department of EntomologyPurdue UniversityWest LafayetteUSA

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