Journal of Chemical Ecology

, Volume 33, Issue 6, pp 1207–1216 | Cite as

Chemotaxis of the Pinewood Nematode, Bursaphelenchus xylophilus, to Volatiles Associated with Host Pine, Pinus massoniana, and its Vector Monochamus alternatus

Article

Abstract

The pinewood nematode (PWN), Bursaphelenchus xylophilus, the most important invasive species in pine forests of Asia, is transported to new pine hosts by beetles of the genus Monochamus. Third-stage dispersal juveniles (JIII) aggregate in pupal chambers around the vector as it matures. We demonstrated that the ratio of three terpenes (α-pinene, β-pinene, and longifolene at 1:2.7:1.1) released by larval Monochamus alternatus strongly attract JIII, whereas the different ratio (1:0.1:0.01) of these three terpenes found in healthy xylem of Pinus massoniana attracts only the propagative stage (Jn) of the nematode. We suggest that the volatiles produced by the host plants could be the basis of a chemoecological relationship between plant parasitic nematodes and their vector insects. Capture of JIII with terpene-baited trap tubes deployed for 2 hr in the field was demonstrated. This technique may lead to the development of rapid sampling methodologies for use at either ports-of-entry or in the field.

Keywords

Aggregation Bursaphelenchus xylophilus Chemo-sensation Location Monochamus alternatus Terpenes 

References

  1. Aikawa, T. and Togashi, K. 1997. An effect of inoculum quantity of Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae) on the nematode load of Monochamus alternatus (Coleoptera: Cerambycidae) in laboratory. Japanese Journal of Nematology 27:14–21.Google Scholar
  2. Boff, M. I. C., van Tol, R. H. W. M., and Smits, P. H. 2002. Behavioural response of Heterorhabditis megidis towards plant roots and insect larvae. BioControl 47:67–83.CrossRefGoogle Scholar
  3. Bolla, J. A., Bramble, J., and Bolla, R. I. 1989. Attraction of Bursaphelenchus xylophilus, Pathotype MPS-1, to Monochamus carolinensis larvae. Japanese Journal of Nematology 19:32–37.Google Scholar
  4. Bruce, T. J., Wadhams, L. J., and Woodcock, C. M. 2005. Insect host location: a volatile situation. Trends Plant Sci. 10:269–274.PubMedCrossRefGoogle Scholar
  5. Dwinell, L. D. 1997. The pinewood nematode: regulation and mitigation. Annu. Rev. Phytopathol. 35:153–166.PubMedCrossRefGoogle Scholar
  6. Futai, K. 1980. Host Preference of Bursaphelenchus lignicolus (Nematoda: Aphelenchoididae) and B. mucronatus shown by their aggregation to pine saps. Appl. Entomol Zool. 15:193–197.Google Scholar
  7. Grewal, P. S., Lewis, E. E., Gaugler, R., and Campbell, J. F. 1994. Host finding behavior as a predictor of foraging strategy in entomopathogenic nematodes. Parasitology 108:207–215.CrossRefGoogle Scholar
  8. Jikumaru, S. and Togashi, K. 2003. Boarding abilities of Bursaphelenchus mucronatus and B. xylophilus (Nematoda: Aphelenchoididae) on Monochamus alternatus (Coleoptera: Cerambycidae). Nematology 5:843–849.CrossRefGoogle Scholar
  9. Kiyohara, T. and Tokushige, Y. 1971. Inoculation experiments of a nematode, Bursaphelenchus sp., onto pine trees. Journal of Japanese Forestry Society 53:210–218.Google Scholar
  10. Kobayashi, F., Yamane, A., and Ikeda, T. 1984. The Japanese pine sawyer beetle as the vector of pine wilt disease. Annu. Rev. Entomol. 29:115–135.CrossRefGoogle Scholar
  11. Lacey, E. S., Ginzel, M. D., Millar, J. G., and Hanks, L. M. 2004. Male-produced aggregation pheromone of the cerambycid beetle Neoclytus acuminatus acuminatus. J. Chem. Ecol. 30:1493–1507.PubMedCrossRefGoogle Scholar
  12. Linit, M. J. 1988. Nematode-vector relationships in the pine wilt disease system. J. Nematol. 20:227–235.PubMedGoogle Scholar
  13. Liebhold, A. M., Macdonald, W. L., Bergdah, D., and Mastro, V. C. 1995. Invasion by exotic forest pests: A threat to forest ecosystems. For. Sci. Monogr. 30:1–49.Google Scholar
  14. Mamiya, Y. 1972. Aggregation of the pinewood nematode around the pupal chamber of the vector beetle. Trans. Annu. Meet. Kanto Branch Jpn. For. Soc. 24:30.Google Scholar
  15. Miyazaki, M., Oda, K., and Yamaguchi, A. 1977. Behavior of Bursaphelenchus lignicolus to unsaturated fatty acids. J. Jpn. Wood. Res. Soc. 23:254–261.Google Scholar
  16. Monti, L., Lalanne-Cassou, B., Lucas, P., Malosse, C., and Silvain, J. F. 1995. Differences in sex pheromone communication systems of closely related species: Spodoptera latifascia (Walker) and S. descoinsi Lalanne-Cassou & Silvain (Lepidoptera: Noctuidae). J. Chem. Ecol. 21:641–660.CrossRefGoogle Scholar
  17. Mota, M. M., Braasch, H., Bravo, M. A., Penas, A. C., Burgermeister, W., Metge, K., and Sousa, E. 1999. First report of Bursaphelenchus xylophilus in Portugal and in Europe. Nematology 1:727–734.CrossRefGoogle Scholar
  18. Necibi, S. and Linit, M. J. 1998. Effect of Monochamus carolinensis on Bursaphelenchus xylophilus dispersal stage formation. J. Nematol. 30:246–254.PubMedGoogle Scholar
  19. Perry, R. N. 1996. Chemoreception in plant parasitic nematodes. Annu. Rev. Phytopathol. 34:181–199.PubMedCrossRefGoogle Scholar
  20. Premachandran, D., von Mende, N., Hussey, R. S., and McClure, M. A. 1988. A method for staining nematode secretions and structures. J. Nematol. 20:70–78.PubMedGoogle Scholar
  21. Rasmann, S., Kollner, T. G., Degenhardt, J., Hiltpold, I., Toepfer, S., Kuhlmann, U., Gershenzon, J., and Turlings, T. C. J. 2005. Recruitment of entomopathogenic nematodes by insect-damaged maize roots. Nature 434:732–737.PubMedCrossRefGoogle Scholar
  22. Rautapaa, J. 1986. Experiences with Bursaphelenchus in Finland. Conference on Pest and Disease Problems in European Forests. EPPO Bull. 16:453–456.CrossRefGoogle Scholar
  23. Schrader, G. and Unger, J. G. 2003. Plant quarantine as a measure against invasive alien species: the framework of the international plant protection convention and the plant health regulations in the European Union. Biological Invasions 5:357–364.CrossRefGoogle Scholar
  24. Sousa, E., Bravo, M. A., Pires, J., Naves, P., Penas, A. C., Bonifacio, L., and Mota, M. M. 2001. Bursaphelenchus xylophilus (Nematoda; Aphelenchoididae) associated with Monochamus galloprovincialis (Coleoptera; Cerambycidae) in Portugal. Nematology 3:89–91.CrossRefGoogle Scholar
  25. Sun, J. H. 2005. Forest invasive species: country report-P.R. China, pp. 80–86, in M. Philip, B. Chris, J. H. Sun, and J. Wu (eds.). The Unwelcome Guests, Proceedings of the Asia-Pacific Forest Invasive Species Conference. FAO Regional Office for Asia and Pacific, Thailand.Google Scholar
  26. Sun, Y. C. 1982. Bursaphelenchus xylophilus was discovered in Sun Yet-sen’s mausoleum in Nanjing. J. Jiangsu For. Sci. Tech. 4:47.Google Scholar
  27. Stamps, W. T. and Linit, M. J. 2001. Integration of intrinsic and extrinsic chemical cues in the behaviour of Bursaphelenchus xylophilus (Aphelenchida: Aphelechoididae) in relation to its beetle vectors. Nematology 3:295–301.CrossRefGoogle Scholar
  28. van Tol, R. W. H. M., van der Sommen, A. T. C., Boff, M. I. C., van Bezooijen, J., Sabelis, M. W., and Smits, P. H. 2001. Plants protect their roots by alerting the enemies of grubs. Ecol. Lett. 4:292–294.CrossRefGoogle Scholar
  29. Yang, B. J., Pan, H. Y., Tang, J., Wang, Y. Y., Wang, L. F., and Wang, Q. 2003. Bursaphelenchus xylophilus. Chinese Forestry Press, Beijing.Google Scholar
  30. Zhao, X., Schmitt, M., and Hawes, M. C. 2000. Species-dependent effects of border cell and root tip exudates on nematode behavior. Phytopathology 90:1239–1245.CrossRefPubMedGoogle Scholar
  31. Zuckerman, B. M. and Jansson, H. B. 1984. Nematode chemotaxis and possible mechanisms of host/prey recognition. Annu. Rev. Phytopathol. 22:95–113.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Li Lin Zhao
    • 1
    • 2
  • Wei Wei
    • 1
  • Le Kang
    • 1
  • Jiang Hua Sun
    • 1
  1. 1.State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of ZoologyThe Chinese Academy of SciencesBeijingPeople’s Republic of China
  2. 2.Graduate University of Chinese Academy of SciencesBeijingPeople’s Republic of China

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