Journal of Chemical Ecology

, Volume 31, Issue 4, pp 829–843 | Cite as

Diet-Related Modification of Cuticular Hydrocarbon Profiles of the Argentine Ant, Linepithema humile, Diminishes Intercolony Aggression

  • Grzegorz Buczkowski
  • Ranjit Kumar
  • Steven L. Suib
  • Jules SilvermanEmail author
Original Article


Territorial boundaries between conspecific social insect colonies are maintained through a highly developed nestmate recognition system modulated by heritable and, in some instances, nonheritable cues. Argentine ants, Linepithema humile, use both genetic and environmentally derived cues to discriminate nestmates from nonnestmates. We explored the possibility that intraspecific aggression in the Argentine ant might diminish when colonies shared a common diet. After segregating recently field-collected colony pairs into high or moderate aggression categories, we examined the effect of one of three diets: two hydrocarbon-rich insect prey, Blattella germanica and Supella longipalpa, and an artificial (insect-free) diet, on the magnitude of aggression loss. Aggression diminished between colony pairs that were initially moderately aggressive. However, initially highly aggressive colony pairs maintained high levels of injurious aggression throughout the study, independent of diet type. Each diet altered the cuticular hydrocarbon profile by contributing unique, diet-specific cues. We suggest that acquisition of common exogenous nestmate recognition cues from shared food sources may diminish aggression and promote fusion in neighboring colonies of the Argentine ant.

Key Words

Argentine ant cuticular hydrocarbons diet invasive ants nestmate recognition unicoloniality 



We thank C. Apperson, C. Schal, E. Vargo, and two anonymous reviewers for helpful comments on the manuscript, G. Blomquist for providing hydrocarbon standards and for technical input, A. Carper for technical assistance, C. Brownie and J. Smith for statistical advice, and D. Dillingham, G. Kirby, and D. Suiter for help in locating ant colonies. This study was supported by the Blanton J. Whitmire Endowment at North Carolina State University.


  1. Bhatkar, A. D., Whitcomb, W. H. 1970Artificial diet for rearing various species of antsFla. Entomol.53229232Google Scholar
  2. Buczkowski, G., Vargo, E. L., Silverman, J. 2004The diminutive supercolony: The Argentine ants of the southeastern United StatesMol. Ecol.1322352242CrossRefPubMedGoogle Scholar
  3. Chen, J. S. C., Nonacs, P. 2000Nestmate recognition and intraspecific aggression based on environmental cues in Argentine ants (Hymenoptera: Formicidae)Ann. Entomol. Soc. Am.9313331337Google Scholar
  4. Foitzik, S., Heinze, J. 1998Nestsite limitation and colony take over in the ant, Leptothorax nylanderiBehav. Ecol.9367375Google Scholar
  5. Gamboa, G. J., Reeve, H. K., Ferguson, I. D., Wacker, T. L. 1986Nestmate recognition in social wasps: The origin and acquisition of recognition odoursAnim. Behav.34685695Google Scholar
  6. Giraud, T., Pedersen, J. S., Keller, L. 2002Evolution of supercolonies: The Argentine ants of southern EuropeProc. Natl. Acad. Sci. USA9960756079CrossRefPubMedGoogle Scholar
  7. Heinze, J., Foitzik, S., Hippert, A., Hölldobler, B. 1996Apparent dear-enemy phenomenon and environment-based recognition cues in the ant Leptothorax nylanderiEthology102510522Google Scholar
  8. Holway, D. A., Suarez, A. V., Case, T. J. 1998Loss of intraspecific aggression in the success of a widespread invasive social insectScience282949952CrossRefPubMedGoogle Scholar
  9. Ichinose, K. 1991Seasonal variation in nestmate recognition in Paratrechina flavipes (Smith) worker ants (Hymenoptera: Formicidae)Anim. Behav.4116Google Scholar
  10. Jutsum, A. R., Saunders, T. S., Cherrett, J. M. 1979Intraspecific aggression in the leaf-cutting ant Acromyrmex octospinosusAnim. Behav.27839844Google Scholar
  11. Jurenka, R. A., Schal, C., Burns, E., Chase, J., Blomquist, G. J. 1989Structural correlation between cuticular hydrocarbons and female contact sex pheromone of German cockroach Blattella germanica (L.)J. Chem. Ecol.15939949Google Scholar
  12. Lahav, S., Soroker, V., Hefetz, A., Meer, R. K. 1999Direct behavioral evidence for hydrocarbons as ant recognition discriminatorsNaturwissenschaften86246249Google Scholar
  13. Langen, T. A., Tripet, F., Nonacs, P. 2000The red and the black: Habituation and the dear-enemy phenomenon in two desert Pheidole antsBehav. Ecol. Sociobiol.48285292Google Scholar
  14. Le Moli, F., Mori, A., Grasso, D. A. 1992Nestmate and conspecific non-nestmate recognition in Formica cunnicularia Latr.: The effect of diet differenceBillen, J. eds. Biology and Evolution of Social InsectsLeuven University PressLeuven, Belgium161165Google Scholar
  15. Liang, D., Silverman, J. 2000“You are what you eat”: Diet modifies cuticular hydrocarbons and nestmate recognition in the Argentine ant, Linepithema humileNaturwissenschaften87412416CrossRefPubMedGoogle Scholar
  16. Liang, D., Blomquist, G. J., Silverman, J. 2001Hydrocarbon-released nestmate aggression in the Argentine ant, Linepithema humile, following encounters with insect preyComp. Biochem. Physiol.129871882Google Scholar
  17. Markin, G. P. 1970The seasonal life cycle of the Argentine ant, Iridomyrmex humilis (Hymenoptera: Formicidae) in southern CaliforniaAnn. Entomol. Soc. Am.6312381242Google Scholar
  18. Newell, W. and Barber, T. C. 1913. The Argentine Ant. USDA Bureau of Entomology Bulletin 122.Google Scholar
  19. Obin, M. S., Meer, R. K. 1988Sources of nestmate recognition cues in the imported fire ant Solenopsis invicta Buren (Hymenoptera: Formicidae)Anim. Behav.3613611370Google Scholar
  20. Roulston, T. H., Buczkowski, G., Silverman, J. 2003Nestmate discrimination in ants: Effect of bioassay on aggressive behaviorInsectes Soc.50151159Google Scholar
  21. SAS Institute2002SAS/STAT Guide for Personal Computers, version 8.1SAS InstituteCary, NCGoogle Scholar
  22. Silverman, J., Liang, D. 2001Colony disassociation following diet partitioning in a unicolonial antNaturwissenschaften887377PubMedGoogle Scholar
  23. Stuart, R. J. 1987Transient nest mate recognition cues contribute to a multicolonial population structure in the ant, Leptothorax curvispinosusBehav. Ecol. Sociobiol.21229235Google Scholar
  24. Stuart, R. J., Herbers, J. M. 2000Nest mate recognition in ants with complex colonies: Within- and between-population variationBehav. Ecol.11676685Google Scholar
  25. Suarez, A. V., Tsutsui, N. D., Holway, D. A., Case, T. J. 1999Behavioral and genetic differentiation between native and introduced populations of the Argentine antBiol. Inv.14353Google Scholar
  26. Suarez, A. V., Holway, D. A., Liang, D., Tsutsui, N. D., Case, T. J. 2002Spatio-temporal patterns of intraspecific aggression in the invasive Argentine antAnim. Behav.64697708Google Scholar
  27. Temeles, E. J. 1994The role of neighbors in territorial systems: When are they “dear enemies?”Anim. Behav.47339350Google Scholar
  28. Tsutsui, N. D., Case, T. J. 2001Population genetics and colony structure of the Argentine ant (Linepithema humile) in its native and introduced rangesEvolution55976985PubMedGoogle Scholar
  29. Tsutsui, N. D., Suarez, A. V., Holway, D. A., Case, T. J. 2000Reduced genetic variation and the success of an invasive speciesProc. Natl. Acad. Sci. USA9759485953PubMedGoogle Scholar
  30. Tsutsui, N. D., Suarez, A. V., Grosberg, R. K. 2003Genetic diversity, asymmetrical aggression, and recognition in a widespread invasive speciesProc. Natl. Acad. Sci. USA10010781083PubMedGoogle Scholar
  31. Meer, R. K., Saliwanchik, D., Lavine, B. 1989Temporal changes in colony cuticular hydrocarbon patterns of Solenopsis invicta: Implications for nestmate recognitionJ. Chem. Ecol.1521152125Google Scholar
  32. Meer, R. K., Morel, L. 1998Nestmate recognition in antsMeer, R. K.Breed, M. D.Espiele, K. E.Winston, M. L. eds. Pheromone Communication in Social InsectsWestview PressBoulder, CO79103Google Scholar
  33. Zar, J. H. 1999Biostatistical AnalysisPrentice HallNew JerseyGoogle Scholar

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Grzegorz Buczkowski
    • 1
    • 3
  • Ranjit Kumar
    • 2
  • Steven L. Suib
    • 2
  • Jules Silverman
    • 1
    Email author
  1. 1.Department of EntomologyNorth Carolina State UniversityRaleighUSA
  2. 2.Department of ChemistryUniversity of ConnecticutStorrsUSA
  3. 3.Department of EntomologyPurdue UniversityWest LafayetteUSA

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