In vitro Cytotoxicity and Antibacterial Activity of Optimized Silver Nanoparticles Against Wound Infectious Bacteria and Their Morphological Studies

  • Balashanmugam PannerselvamEmail author
  • Tamil Selvi AlagumuthuEmail author
  • Senthil Kumar Cinnaiyan
  • Naif Abdullah Al-Dhabi
  • Karuppiah Ponmurugan
  • Muthupandian Saravanan
  • Swarna V. Kanth
  • Kalaichelvan Pudupalayam Thangavelu
Original Paper


The present study reports the optimized silver nanoparticles (AgNPs). They were tested against, three wound infecting gram-positive and gram-negative pathogenic bacteria viz., B. subtilis, S. aureus, M. luteus, E. coli, K. pneumoniae and P. aeruginosa at 10 µg concentration. In the antibacterial activity of optimized AgNPs, P. aeruginosa was found remarkably sensitive to the AgNPs with 20.3 ± 1.86 mm of inhibition zone. The standard antibiotics of streptomycin, gentamycin, ampicillin and erythromycin at 10 µg, when tested against the bacteria, which revealed that gentamycin showed high antibacterial activity against all the six wound infecting pathogenic bacteria. The MIC and MBC concentrations evaluated against the bacteria showed were 4.0 ± 1.00 µg/mL and 6.3 ± 0.47 µg/mL for S. aureus, respectively. The normal morphology of tested bacteria was changed when treated with optimized AgNPs as evidenced under TEM analysis. The optimized AgNPs impregnated on cotton fabrics showed high antibacterial activity against tested bacteria. Thereafter there was a gradual decrement in antibacterial activity against the bacteria at 2nd, 3rd and 4th wash. The cytotoxicity effect of optimized AgNPs treated normal cell (Vero) recorded a maximum IC50 value 20 µg/mL. Our research outcome opens up new improved antimicrobial composition in pharmaceutical and medical sectors.


Optimized AgNPs Wound infectious bacteria Antibacterial activity AgNPs coated fabric Cytotoxicity 



The authors would like to thank the Director, Centre for Advanced Studies in Botany, University of Madras, Chennai, and CSIR- Central Leather Research Institute (CLRI), Chennai, for providing the laboratory facilities. We thank NCN-SNT, University of Madras, for providing HRTEM, EDX and SAED analysis. We thank The Head, Centre for Nanoscience and Technology, Anna University for AFM analyses. We also thank the University Grants Commission-Bioscience Research (UGC-BSR) (No.F.4-1/2006/(BSR)5-61/2007(BSR) dt 29 Jun 2012.) herbal science scheme, New Delhi.

Compliance with Ethical Standards

Conflicts of interest

The authors report no conflicts of interest in performing this work.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Balashanmugam Pannerselvam
    • 1
    • 2
    Email author
  • Tamil Selvi Alagumuthu
    • 1
    Email author
  • Senthil Kumar Cinnaiyan
    • 1
  • Naif Abdullah Al-Dhabi
    • 3
  • Karuppiah Ponmurugan
    • 3
  • Muthupandian Saravanan
    • 4
  • Swarna V. Kanth
    • 1
  • Kalaichelvan Pudupalayam Thangavelu
    • 2
  1. 1.Centre for Human & Organizational Resources Development (CHORD)CSIR-Central Leather Research InstituteChennaiIndia
  2. 2.Centre for Advanced Studies in BotanyUniversity of MadrasChennaiIndia
  3. 3.Department of Botany and Microbiology, College of ScienceKing Saud UniversityRiyadhSaudi Arabia
  4. 4.Department of Microbiology and Immunology, Institute of Biomedical Sciences, College of Health ScienceMekelle UniversityMekelleEthiopia

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