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Comparative Studies on Functionalization of Bacterial Magnetic Nanoparticles for Drug Delivery

  • Varalakshmi Raguraman
  • K. SuthindhiranEmail author
Original Paper
  • 24 Downloads

Abstract

The study demonstrates the use of bacterial magnetic nanoparticles (BMNPs) as a drug carrier for the anticancer drug. BMNPs extracted from Magnetospirillum gryphiswaldense MSR-1, characterized using microscopic and spectroscopic methods. Drug conjugates were developed by direct binding of crizotinib with lipid membrane of BMNPs (CM) and also by using crosslinkers such as glutaraldehyde (CMG) and 3-aminopropyltriethoxysilane (APTES) (CMA). The developed conjugates were characterized using microscopic and spectroscopic methods. Drug loading capacity was 670 µg/ml for CM and 162.08 µg/ml and 243.15 µg/ml for CMG and CMA respectively. The drug loading efficiency of drug conjugates CM, CMG and CMA were found to be 67%, 16.2% and 24.3%. The drug release assay revealed slow and stable discharge of crizotinib from CM conjugate (8.2%) compared to CMG (88.33%) and CMA (58.46%) at 48 h. The cytotoxicity of CM was found to be high in comparison with BMNPs, CMG, CMA and crizotinib as tested on cell lines: A549, MCF-7 and HeLa. Our study establishes that the direct attachment of crizotinib with BMNPs shows better coupling with higher loading efficiency and long-term release compared to use of linkers. Further studies are needed to validate the results using animal models and also for targeted drug delivery.

Keywords

BMNPs Crizotinib BMNPs-drug conjugates Drug delivery Glutaraldehyde APTES 

Abbreviations

BMPs

Bacterial magnetic nanoparticles

MTB

Magnetotactic bacteria

DSMZ

Deutsche Sammlung von Mikroorganismen und Zellkulturen

MSGM

Magnetospirillum growth medium

FTIR

Fourier-transform infrared spectroscopy

HRTEM

High-resolution transmission electron microscopy

XRD

X-ray diffraction

TGA

Thermogravimetric analysis

AFM

Atomic force microscopy

CM

Crizotinib-magnetosome complex

CMG

Crizotinib-magnetosome complex prepared via glutaraldehyde

CMA

Crizotinib-magnetosome complex prepared via APTES

Notes

Acknowledgements

This work was supported by VIT University. The authors thank the management for providing the facilities for the research.

Compliance with Ethical Standards

Conflict of interest

There is no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Marine Biotechnology and Bioproducts Laboratory, School of Biosciences and TechnologyVellore Institute of TechnologyVelloreIndia

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