Biomimetic Green Synthesis and Characterization of Nanoparticles from Leave Extract of Lavatera cretica and Their Improving Glucose Bigotry

  • Chinnadurai VeeramaniEmail author
  • Mohammed A. Alsaif
  • Khalid S. Al-Numair
Original Paper


This study aims to examine the biosynthesis and characterization of silver nanoparticles using Lavatera cretica (LCAgNPs) leaf extract mixed with silver nitrate and improving their glucose bigotry. The biosynthesis was first confirmed by the color change from colorless (metal salt solution) to brown (nanoparticle colloidal dispersion). The occurrence of the nanoparticles was further ascertained by several physicochemical studies including UV–Vis spectroscopy, transmission electron microscopy (TEM), energy dispersive X-ray (EDX) tomography, Fourier transform-infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The UV–Vis spectroscopy of LCAgNPs indicated the surface plasmon resonance signature of Ag NPs at around 440 nm. The TEM analysis revealed a well-dispersed sphere that varied in size from 5 to 24 nm. The EDX results established that Ag was the major element and others such as C, O and Cl were also present, which specified that the biomolecules coincided the AgNPs. The FT-IR spectroscopic study exhibited that the functional groups such as O–H, C–O, N–H, C–H and C=O were accountable for the synthesis of AgNPs. The XRD pattern represented the crystalline nature of metallic Ag. In this results, it could be clinched that the silver nitrate was reduced to silver nanoparticles of small size and high stability, which were devoid of impurities. Hence, this study has proved that L. cretica leaf is a good source for the synthesis of AgNPs and their improved glucose bigotry.


Nanoparticles XRD EDX TEM Insulin insensitivity LCAg 



The authors would like to extend their sincere appreciation to the Deanship of Scientific Research at King Saud University for its funding of this research through the Research Group Project No. RGP-249.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. 1.
    N. Manosalva, G. Tortella, M. Cristina Diez, H. Schalchli, A. B. Seabra, N. Durán, and O. Rubilar (2019). World J. Microbiol. Biotechnol.35, (6), 88.CrossRefGoogle Scholar
  2. 2.
    C. P. Gong, S. C. Li, and R. Y. Wang (2018). J. Photochem. Photobiol. B.183, 137–141.CrossRefGoogle Scholar
  3. 3.
    N. Atale, S. Saxena, J. G. Nirmala, R. T. Narendhirakannan, S. Mohanty, and V. Rani (2016). Appl. Biochem. Biotechnol.181, (3), 1140–1154.CrossRefGoogle Scholar
  4. 4.
    World Health Organization (WHO). Global Report on Diabetes WHO (Switzerland, Geneva, 2016), p. 2016.Google Scholar
  5. 5.
    K. Chen, A. Jih, O. Osborn, S. T. Kavaler, W. Fu, R. Sasik, R. Saito, and J. J. Kim (2018). Physiol. Genomics50, 144–157.CrossRefGoogle Scholar
  6. 6.
    F. F. Mo, H. X. Liu, Y. Zhang, J. Hua, D. D. Zhao, T. An, D. W. Zhang, T. Tian, and S. H. Gao (2019). Iran J. Basic Med. Sci.22, (3), 262–266.PubMedPubMedCentralGoogle Scholar
  7. 7.
    F. Keshavarzi and S. Golsheh (2019). Mol. Genet. Genomic Med.7, (5), e631.CrossRefGoogle Scholar
  8. 8.
    S. Kalakotla, N. Jayarambabu, G. K. Mohan, R. B. S. M. N. Mydin, and V. R. Gupta (2019). Colloids Surf. B. Biointerfaces174, 199–206.CrossRefGoogle Scholar
  9. 9.
    S. Ben-Nasr, S. Aazza, W. Mnif, and M. G. Miguel (2015). Pharmacogn. Mag.11, 48–54.CrossRefGoogle Scholar
  10. 10.
    L. Viegi, A. Pieroni, P. M. Guarrera, and R. Vangelisti (2003). J. Ethnopharmacol.89, 221–244.CrossRefGoogle Scholar
  11. 11.
    C. Veeramani, M. A. Alsaif, and K. S. Al-Numair (2018). Biomed. Pharmacother.106, 183–191.CrossRefGoogle Scholar
  12. 12.
    D. Singh, V. Kumar, E. Yadav, N. Falls, M. Singh, U. Komal, and A. Verma (2018). IET Nanobiotechnol.12, (6), 748–756.CrossRefGoogle Scholar
  13. 13.
    P. Trinder (1969). J. Clin. Pathol.22, 246.CrossRefGoogle Scholar
  14. 14.
    W. Bürgi, M. Briner, N. Franken, and A. C. Kessler (1988). Clin. Biochem.21, 311–314.CrossRefGoogle Scholar
  15. 15.
    D. R. Matthews, J. P. Hosker, A. S. Rudenski, B. A. Naylor, D. F. Treacher, and R. C. Turner (1984). Diabetologia28, 412–419.CrossRefGoogle Scholar
  16. 16.
    P. Chomczynski and N. Sacchi (1987). Anal. Biochem.162, (1), 156–159.CrossRefGoogle Scholar
  17. 17.
    K. J. Livak and T. D. Schmittgen (2001). Methods25, 402–408.CrossRefGoogle Scholar
  18. 18.
    R. Norouzirad, H. Gholami, M. Ghanbari, M. Hedayati, P. González-Muniesa, S. Jeddi, and A. Ghasemi (2019). Life Sci.3205, (19), 30424–30432.Google Scholar
  19. 19.
    H. Adela and B. H. Frank (2015). Pharmacoeconomics33, (7), 673–689.CrossRefGoogle Scholar
  20. 20.
    A. Tang, A. C. F. Coster, K. T. Tonks, L. K. Heilbronn, N. Pocock, L. Purtell, M. Govendir, J. B. Lythe, J. Zhang, A. Xu, D. J. Chisholm, N. A. Johnson, J. R. Greenfield, and D. Samocha-Bonet (2019). J. Clin. Med.8, (5), 623–644.CrossRefGoogle Scholar
  21. 21.
    I. Gabriely, X. H. Ma, X. M. Yang, G. Atzmon, M. W. Rajala, A. H. Berg, P. Scherer, L. Rossetti, and N. Barzilai (2002). Diabetes51, (10), 2951–2958.CrossRefGoogle Scholar
  22. 22.
    C. Veeramani, M. A. Alsaif, and K. S. Al-Numair (2017). Biomed. Pharmacother.96, 1349–1357.CrossRefGoogle Scholar
  23. 23.
    X. Huang, G. Liu, J. Guo, and Z. Su (2018). Int. J. Biol. Sci.14, (11), 1483–1496.CrossRefGoogle Scholar
  24. 24.
    J. Wang, X. Hu, W. Ai, F. Zhang, K. Yang, L. Wang, X. Zhu, P. Gao, G. Shu, Q. Jiang, and S. Wang (2017). Biochem. Biophys. Res. Commun.489, (4), 432–438.CrossRefGoogle Scholar
  25. 25.
    C. Kim, J. Lee, M. B. Kim, and J. K. Hwang (2018). Food Sci. Biotechnol.28, (3), 895–905.CrossRefGoogle Scholar
  26. 26.
    X. Guo, W. Sun, G. Luo, L. Wu, G. Xu, D. Hou, Y. Hou, X. Guo, X. Mu, L. Qin, and T. Liu (2019). FEBS Open Bio.9, (5), 1008–1019.CrossRefGoogle Scholar
  27. 27.
    X. Li, X. Li, G. Wang, Y. Xu, Y. Wang, R. Hao, and X. Ma (2018). Front. Med.12, (6), 688–696.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Chinnadurai Veeramani
    • 1
    Email author
  • Mohammed A. Alsaif
    • 1
  • Khalid S. Al-Numair
    • 1
  1. 1.Department of Community Health Sciences, College of Applied Medical SciencesKing Saud UniversityRiyadhSaudi Arabia

Personalised recommendations