Synthesis, Characterization and Evaluation of Liponiosome Containing Ginger Extract as a New Strategy for Potent Antifungal Formulation

  • Mahya Mohammadi
  • Bibi Fatemeh Haghirosadat
  • Mohaddeseh larypoor
  • Raheleh Ehsani
  • Fatemeh YazdianEmail author
  • Hamid RashediEmail author
  • Shabnam Jahanizadeh
  • Anosheh Rahmani
Original Paper


Aflatoxins are natural fungous toxins made by Aspergillus species. The toxins can cause tissue necrosis. In this study, nanoliponiosomes containing ginger extract were synthetized and their antifungal effect on the growth and Aflatoxin production of Aspergillus flavus and Aspergillus parasiticus fungi was studied. Physiochemical characteristics of the sample were determined using XRD, FTIR, AFM, DLS and SEM. The resultant nanostructure can operation as an angiogenesis promoter and provide antifungal nanocarrier for improving the wound healing process. Antifungal activity of ginger extract was determined by disc diffusion and microplate dilution methods. The inhibitory effect of extract on Aflatoxin-B1 production was investigated using MIC method. Ginger concentration, cholesterol%, SPAN-60 and DPPC% were optimized by response surface method. FTIR analysis showed that ginger extract and nanoliponiosome had no chemical interaction resulting in the change of functional groups. SEM observation showed the spherical morphology of particles and the average size of 95 nm. It was also found out that ginger extract had a stronger antifungal effect against Aspergillus flavus fungus compared to Aspergillus parasiticus fungus. The results of this study showed that the ginger extract loaded nanoliponiosomes can be promising antifungal agents for improving wound healing process with high antifungal effects and low side effects.


Nanoliponiosome Ginger extract Aspergillus flavus Wound healing Aflatoxin-B1 



  1. 1.
    T. J. Shaw and P. Martin (2009). J. Cell. Sci.122, 3209.PubMedPubMedCentralCrossRefGoogle Scholar
  2. 2.
    A. C. Oliveira Gonzalez, T. Fortuna Costa, Z. D. Araújo Andrade, and A. R. A. Medrado (2016). Bras. Dermatol.91, 614.CrossRefGoogle Scholar
  3. 3.
    M. Moosavian, M. Darvishnia, and H. A. Khosravinia (2017). J. Appl. Res. Plant. Protect.6, 37.CrossRefGoogle Scholar
  4. 4.
    Z. Salehi, B. Kheir Khah, Z. Masoumalinejad, and M. R. Zinatizadeh (2018). Iran J. Med. Microbiol.12, 43.Google Scholar
  5. 5.
    A. A. Ismaiel, G. H. Rabie, S. E. Kenawey, and M. A. Abd El-Aal (2012). Braz. J. Microbiol.43, 1355.PubMedPubMedCentralCrossRefGoogle Scholar
  6. 6.
    A. Naseri, P. Arj, M. J. Najaf, and H. Rakhshandehzadeh (2015). J. Birjand. Univ. Med. Sci.22, 115.Google Scholar
  7. 7.
    N. J. Toyang and R. Verpoorte (2013). J. Ethno. pharmacol.146, 681.CrossRefGoogle Scholar
  8. 8.
    S. Ghaderi, A. Falahati-HosseinAbad, M. H. Sarailoo, and V. Ghanbari (2012). J. Shahrekord Univ. Medic. Sci.14, 74.Google Scholar
  9. 9.
    M. Salem and Y. Zidan (2015). Int. Biodeterior Biodegred.106, 96.Google Scholar
  10. 10.
    M. Sadrnia (2018). Arak. Med. Univ. J.21, 63.Google Scholar
  11. 11.
    M. Abdolmaleki, S. Bhramynzhad, S. Abbasi, and B. Mahmoudi (2010). J. Sugar. Beet.25, 193.Google Scholar
  12. 12.
    A. Behbehani, F. Tabatabaei Yazdi, F. Shahidi, and M. Mohebbi (2013). J. Rafsanjan. Univ. Med. Sci.12, 1015.Google Scholar
  13. 13.
    Z. Atai, M. Ansari, A. Mousavi, and A. Mirzaei (2007). J. Islam. Den. Associat.19, 91.Google Scholar
  14. 14.
    M. Mohammadi, S. J. Hashemi, S. Rezaie, and M. Bayat (2018). J. Microbi. World.11, 88.Google Scholar
  15. 15.
    W. Abdallah and E. Abdallah (2018). Int. J. Pharm. Chin. Med.2, 1.CrossRefGoogle Scholar
  16. 16.
    D. Marion, S. Bourgeois, V. Andrieu, and H. Fessi (2018). Pharma.10, 10.Google Scholar
  17. 17.
    N. Tabassum (2019). J. Tradit. Med. Clin. Nature8, 1.Google Scholar
  18. 18.
    G. Tiwari, R. Tiwari, B. Sriwastawa, L. Bhati, S. Pandey, and S. K. Bannerjee (2012). Int. J. Pharm. Invest.2, 2.CrossRefGoogle Scholar
  19. 19.
    H. Daraee, A. Etemadi, M. Kouhi, S. Alimirzalu, and A. Akbarzadeh (2016). Artific Cell Nanomed. Biotech.44, 381.Google Scholar
  20. 20.
    M. Cheraghi, B. Negahdari, H. Daraee, and A. Etemadi (2017). Pharmacotherap.86, 316.CrossRefGoogle Scholar
  21. 21.
    F. Haghiralsadat, G. Amoabediny, M. H. Sheikhha, T. Forouzanfar, M. N. Helder, and B. A. Zandieh-doulabi (2017). Cell. J.19, 55.PubMedPubMedCentralGoogle Scholar
  22. 22.
    S. Ambati, A. R. Ferarro, S. E. Kang, J. Lin, X. Lin, M. Momany, Z. A. Lewis, and R. B. Meagher (2019). mSphere.4, 1.Google Scholar
  23. 23.
    G. Amoabediny, F. Haghiralsadat, S. Naderinezhad, M.N. Helder, E. Akhoundi Kharanaghi, J. Mohammadnejad Arough, and B. Zandieh-Doulabi (2018). Int. J. Poly. Mat. Poly. Biomat.67, 383e400.Google Scholar
  24. 24.
    R. Muzzalupo and L. Tavano (2015). Res. Rep. Transderm. Drug. Deliv.4, 23.Google Scholar
  25. 25.
    S. Naderinezhad, F. Haghiralsadat, G. Amoabediny, S. R. Najafabadi, and A. Akbarzade (2017). New. Cell. Mol. Biotechnol. J.8, 17.Google Scholar
  26. 26.
    W. Gao, Y. Chen, Y. Zhang, Q. Zhang, and L. Zhang (2018). Adv. Drug. Deliv. Rev.127, 46.PubMedCrossRefGoogle Scholar
  27. 27.
    I. F. Almeida, H. M. Martins, S. M. Santos, M. S. Freitas, and J. M. da Costa (2011). Toxins (Basel).3, 163.PubMedPubMedCentralCrossRefGoogle Scholar
  28. 28.
    A. Abdel-Hadi, M. Schmidt-Heydt, R. Parra, R. Geisen, and N. Magan (2012). J. Roy. Soc. Interfac.9, 757.CrossRefGoogle Scholar
  29. 29.
    H. Johari, E. Sharifi, F. Delirnasab, V. Hemayatkhah, H. Kargar, and M. Nikpoor (2013). J. Rafsanjan Uni. Med. Sci.12, 417.Google Scholar
  30. 30.
    F. Haghiralsadat, G. Amouabedini, S. Naderinezhad, M. H. Sheikhha, Z. Malaei-balasi, and A. Akbaezadeh (2017). New. Cell. Mol. Biotechnol. J.7, 49.Google Scholar
  31. 31.
    B. Noorani, F. Tabandeh, F. Yazdian, Z. S. Soheili, M. Shakibaie, and Sh Rahmani (2018). Int. J. Poly. Mat.67, 754.CrossRefGoogle Scholar
  32. 32.
    A. I. El-Batal, Gh S El-Sayyad, A. El-Ghamery, and M. Gobara (2017). J. Cluster. Sci.28, 1083.CrossRefGoogle Scholar
  33. 33.
    D. Garcia, A. J. Ramos, V. Sanchis, and S. MarÌn (2013). Int. J. Food. Microbiol.162, 182.PubMedCrossRefGoogle Scholar
  34. 34.
    R. M. Coopoosamy and M. L. Magwa (2007). Afr. J. Biotechnol.6, 2406.CrossRefGoogle Scholar
  35. 35.
    D. Jasso de Rodriguez and R. Rodriguez-Gracia (2005). Indust. Crops. Produc.21, 81.CrossRefGoogle Scholar
  36. 36.
    M. Majdizadeh, S. Zarchi, A. A. Movahedpour, H. Malmir, E. Sasani, and B. F. Haghiralsadat (2018). J. Shahid Sadoughi Univ. Med. Sci.25, 853.Google Scholar
  37. 37.
    R. Nasirzadeh, B. Ghanbarzadeh (2016). Antioxidant investigation of Ginger loaded nanoniosome, Tabriz University, Thesis. Guided by Proff. Ghanbarzadeh.Google Scholar
  38. 38.
    X. Zhao, H. Zhu, J. Chen, and Q. Ao (2015). J. Food. Process. Preserv.39, 2017.CrossRefGoogle Scholar
  39. 39.
    Ch I Nkanga, R. W. Krause, X. S. Noundou, and R. B. Walker (2017). Int. J. Pharmaceutic526, 466.CrossRefGoogle Scholar
  40. 40.
    H. Lei, Q. Wei, Q. Wang, A. Su, M. Xue, Q. Liu, and Q. Hu (2017). Mat. Sci. Eng. C73, 381.CrossRefGoogle Scholar
  41. 41.
    Sh Jahanizadeh, F. Yazdian, A. Marjani, M. Omidi, and H. Rashedi (2017). Int. J. Biol. Macro. Mole.105, 757.CrossRefGoogle Scholar
  42. 42.
    P. R. de Moraes, S. Saska, H. Barud, and L. R. de Lima (2016). Mat. Res.19, 1.CrossRefGoogle Scholar
  43. 43.
    E. Sasani and H. Sh (2018). J. Shahid. Sadoughi. Univ. Med. Sci.26, 528.Google Scholar
  44. 44.
    Sh Aghayan, S. Zaker, and M. Shahla’i (2017). J. Res. Dentis.4, 27.Google Scholar
  45. 45.
    N. Shoaie, P. Mohammadi, and S. Mohammadi (2012). Armaghan Danesh.17, 1.Google Scholar
  46. 46.
    M. Mahboubi (2019). Clin. Phytosci.5, 1.CrossRefGoogle Scholar
  47. 47.
    M. Aghazadeh, A. Bialvaei, M. Aghazadeh, F. Kabiri, N. Saliani, M. Yousefi, H. Eslami, and H. S. Kafil (2016). Jundishapur. J Microbiol.9, 1.Google Scholar
  48. 48.
    T. Chen, J. Lu, B. Kang, M. Lin, L. Ding, L. Zhang, G. Chen, Sh Chen, and H. Lin (2018). Front. Microbiol.9, 1.CrossRefGoogle Scholar
  49. 49.
    M. H. Haghighi and A. R. Khosravi (2013). J. Babol. Univ. Med. Sci.15, 2.Google Scholar
  50. 50.
    A. Gran, B. Salehnia, H. Alizadeh, M. Farzaneh, and M. Shivazad (2016). J. Veterinary Res.70, 1.Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Mahya Mohammadi
    • 1
  • Bibi Fatemeh Haghirosadat
    • 2
    • 3
  • Mohaddeseh larypoor
    • 4
  • Raheleh Ehsani
    • 2
  • Fatemeh Yazdian
    • 5
    Email author
  • Hamid Rashedi
    • 6
    Email author
  • Shabnam Jahanizadeh
    • 7
  • Anosheh Rahmani
    • 8
  1. 1.Department of Biotechnology, Tehran North BranchIslamic Azad UniversityTehranIran
  2. 2.Medical Nanotechnology & Tissue Engineering Research Center, Yazd Reproductive Sciences InstituteShahid Sadoughi University of Medical SciencesYazdIran
  3. 3.Department of Advanced Medical Sciences and Technologies, School of ParamedicineShahid Sadoughi University of Medical SciencesYazdIran
  4. 4.Department of Biotechnology, Tehran North BranchIslamic Azad UniversityTehranIran
  5. 5.Department of Life Science Engineering, Faculty of New Science and TechnologiesUniversity of TehranTehranIran
  6. 6.Department of Biotechnology, School of Chemical Engineering, College of EngineeringUniversity of TehranTehranIran
  7. 7.Young Researchers and Elite Club, Arak BranchIslamic Azad UniversityArakIran
  8. 8.Scientific Member and Assistant Professor of Food and Agriculture FacultyStandard Research Institute (SRI)KarajIran

Personalised recommendations