MnO2 Nanoparticles and Carbon Nanofibers Nanocomposites with High Sensing Performance Toward Glucose
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By combining the advantages of manganese dioxide nanoparticles (MnO2 NPs) and carbon nanofibers (CNFs), a biosensing electrode surface as a high-performance enzyme biosensor is designed in this work. MnO2 NPs and CNFs nanocomposites (MnO2–CNFs) were prepared by using a simple hydrothermal method and then were characterized by scanning electron microscopy, powder X-ray diffraction, fourier transform infrared spectroscopy, energy dispersive spectrometry and electrochemisty. The results showed that MnO2 NPs are uniformly attached to the surface of CNFs. Meanwhile, the MnO2–CNFs nanocomposites as a supporting matrix can provide an efficient and advantageous platform for electrochemical sensing applications. On the basis of the improved sensitivity of MnO2–CNFs modified electrode toward H2O2 at low overpotential, a MnO2–CNFs based glucose biosensor was fabricated by monitoring H2O2 produced by an enzymatic reaction between glucose oxidase and glucose. The constructed biosensor exhibited a linear calibration graph for glucose in a concentration range of 0.08–4.6 mM and a low detection limit of 0.015 mM. In addition, the biosensor showed other excellent characteristics, such as high sensitivity and selectivity, short response time, and the relative low apparent Michaelis–Menten constant. Analysis of human urine spiked with glucose at different concentration levels yielded recoveries between 101.0 and 104.8%.
KeywordsMnO2–CNFs nanocomposites Hydrogen peroxide Glucose oxidase Glucose
The authors thank the National Natural Science Foundation of China (21001004), the Natural Science Foundation of the Anhui Higher Education Institutions (Grant No. KJ2016A277), the Innovation Funds of Anhui Normal University (Grant 741606), Ph.D. Research Startup Funds of Anhui Normal University (2018XJJ-751862), the Key Laboratory of Functional Molecular Solids, Ministry of Education and Anhui Laboratory of Molecule-Based Materials (16005) and the Training Programs of Innovation and Entrepreneurship for Undergraduates (201610370471).
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