Journal of Cluster Science

, Volume 28, Issue 1, pp 565–580 | Cite as

Seagrasses as Sources of Mosquito Nano-Larvicides? Toxicity and Uptake of Halodule uninervis-Biofabricated Silver Nanoparticles in Dengue and Zika Virus Vector Aedes aegypti

  • Jazem A. Mahyoub
  • Al Thabiani Aziz
  • Chellasamy Panneerselvam
  • Kadarkarai Murugan
  • Mathath Roni
  • Subrata Trivedi
  • Marcello Nicoletti
  • Usama W. Hawas
  • Fekri M. Shaher
  • Muneer A. Bamakhrama
  • Angelo Canale
  • Giovanni BenelliEmail author
Original Paper


Mosquitoes (Diptera: Culicidae) act as vectors for devastating pathogens and parasites. Zika virus, an Aedes mosquito-borne flavivirus, is becoming a worldwide public health concern following its suspected association with over 4000 recent cases of microcephaly in the infants of some women who were pregnant when they contracted the disease. There are no specific treatments for Zika virus, thus the eco-friendly and effective control of mosquito vectors is crucial. This research proposed a novel method of seagrass-mediated synthesis of silver nanoparticles (AgNP) using Halodule uninervis as a reducing and capping agent. UV–Vis spectroscopy, FTIR spectroscopy, SEM, EDX spectroscopy, XRD and Raman analysis confirmed the rapid and cheap synthesis of AgNP. LC50 of H. uninervis extract against Aedes aegypti was 295.629 ppm and LC50 of H. uninervis-synthesized AgNP was 12.554 ppm. Microscopy analysis pointed out the uptake of H. uninervis-fabricated AgNP in the midgut of mosquito larvae. In MIC assays, low doses of the AgNP inhibited the growth of Bacillus subtilis, Klebsiella pneumoniae and Salmonella typhi. Overall, this research shed light on the mosquitocidal potential of H. uninervis, as a bio-resource for the cheap and effective nanosynthesis of mosquitocidal biopesticides.


Arbovirus Yellow fever West Nile Nanotechnology Multi-drug resistant bacteria 



Dr. Jazem A. Mahyoub is grateful to the Deanship of Scientific Research (DSR) at King Abdulaziz University, Jeddah, Grant No. G-256-130-37. The authors would like to extend their sincere appreciation to the Deanship of Scientific Research for its technical and financial support.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Jazem A. Mahyoub
    • 1
    • 2
  • Al Thabiani Aziz
    • 3
  • Chellasamy Panneerselvam
    • 3
  • Kadarkarai Murugan
    • 4
    • 5
  • Mathath Roni
    • 4
  • Subrata Trivedi
    • 3
  • Marcello Nicoletti
    • 6
  • Usama W. Hawas
    • 7
  • Fekri M. Shaher
    • 7
  • Muneer A. Bamakhrama
    • 7
  • Angelo Canale
    • 8
  • Giovanni Benelli
    • 8
    Email author
  1. 1.Department of Biology Sciences, Faculty of SciencesKing Abdulaziz UniversityJeddahSaudi Arabia
  2. 2.IBB UniversityIbbRepublic of Yemen
  3. 3.Biology Department, Faculty of ScienceUniversity of TabukTabukSaudi Arabia
  4. 4.Division of Entomology, Department of Zoology, School of Life SciencesBharathiar UniversityCoimbatoreIndia
  5. 5.Thiruvalluvar UniversityVelloreIndia
  6. 6.Department of Environmental BiologySapienza University of RomeRomeItaly
  7. 7.Department of Marine Chemistry, Faculty of Marine SciencesKing Abdulaziz UniversityJeddahSaudi Arabia
  8. 8.Department of Agriculture, Food and EnvironmentUniversity of PisaPisaItaly

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