Advertisement

Journal of Cluster Science

, Volume 28, Issue 1, pp 565–580 | Cite as

Seagrasses as Sources of Mosquito Nano-Larvicides? Toxicity and Uptake of Halodule uninervis-Biofabricated Silver Nanoparticles in Dengue and Zika Virus Vector Aedes aegypti

  • Jazem A. Mahyoub
  • Al Thabiani Aziz
  • Chellasamy Panneerselvam
  • Kadarkarai Murugan
  • Mathath Roni
  • Subrata Trivedi
  • Marcello Nicoletti
  • Usama W. Hawas
  • Fekri M. Shaher
  • Muneer A. Bamakhrama
  • Angelo Canale
  • Giovanni BenelliEmail author
Original Paper

Abstract

Mosquitoes (Diptera: Culicidae) act as vectors for devastating pathogens and parasites. Zika virus, an Aedes mosquito-borne flavivirus, is becoming a worldwide public health concern following its suspected association with over 4000 recent cases of microcephaly in the infants of some women who were pregnant when they contracted the disease. There are no specific treatments for Zika virus, thus the eco-friendly and effective control of mosquito vectors is crucial. This research proposed a novel method of seagrass-mediated synthesis of silver nanoparticles (AgNP) using Halodule uninervis as a reducing and capping agent. UV–Vis spectroscopy, FTIR spectroscopy, SEM, EDX spectroscopy, XRD and Raman analysis confirmed the rapid and cheap synthesis of AgNP. LC50 of H. uninervis extract against Aedes aegypti was 295.629 ppm and LC50 of H. uninervis-synthesized AgNP was 12.554 ppm. Microscopy analysis pointed out the uptake of H. uninervis-fabricated AgNP in the midgut of mosquito larvae. In MIC assays, low doses of the AgNP inhibited the growth of Bacillus subtilis, Klebsiella pneumoniae and Salmonella typhi. Overall, this research shed light on the mosquitocidal potential of H. uninervis, as a bio-resource for the cheap and effective nanosynthesis of mosquitocidal biopesticides.

Keywords

Arbovirus Yellow fever West Nile Nanotechnology Multi-drug resistant bacteria 

Notes

Acknowledgements

Dr. Jazem A. Mahyoub is grateful to the Deanship of Scientific Research (DSR) at King Abdulaziz University, Jeddah, Grant No. G-256-130-37. The authors would like to extend their sincere appreciation to the Deanship of Scientific Research for its technical and financial support.

References

  1. 1.
    H. Mehlhorn, K. A. Al-Rasheid, S. Al-Quraishy, and F. Abdel-Ghaffar (2012). Parasitol. Res. 110, 259.CrossRefGoogle Scholar
  2. 2.
    R. Rajaganesh, K. Murugan, C. Panneerselvam, S. Jayashanthini, A. T. Aziz, M. Roni, U. Suresh, S. Trivedi, H. Rehman, A. Higuchi, M. Nicoletti, and G. Benelli (2016). Fern-synthesized silver nanocrystals: towards a new class of mosquito oviposition deterrents? Res. Vet. Sci. 109, 40–51.CrossRefGoogle Scholar
  3. 3.
    G. Benelli (2016). Asian Pac. J. Trop. Biomed. 6, 468.CrossRefGoogle Scholar
  4. 4.
    G. Benelli (2016). Parasitol. Res. 115, 23.CrossRefGoogle Scholar
  5. 5.
    G. Benelli (2016). Asia Pacif. J. Trop. Biomed. 6, 353–354.CrossRefGoogle Scholar
  6. 6.
    Global Alert and Response (2015) Global alert and response—impact of dengue. http://www.who.int/csr/disease/dengue/impact/en/ Accessed 27.07.15.
  7. 7.
    D. Musso and D. J. Gubler (2016). Clin. Microbiol. Rev. 29, 487.CrossRefGoogle Scholar
  8. 8.
    A. J. Rodriguez-Morales (2015). J. Infect. Dev. Ctries 9, 684.CrossRefGoogle Scholar
  9. 9.
    G. Savidis, W. M. McDougall, Perreira J. M. MeranerP, Trincucci G. Portmann, S. P. John, A. M. Aker, N. Renzette, D. R. Robbins, Z. Guo, S. Green, T. F. Kowalik, and A. L. Brass (2016). Cell Rep. 16, 232.CrossRefGoogle Scholar
  10. 10.
    European Centre for Disease Prevention and Control (2015) Zika virus infection: factsheet for health professionals. http://ecdc.europa.eu/en/healthtopics/zika_virus_infection/factsheet-health-professionals/Pages/factsheet_health_professionals.aspx
  11. 11.
    R. S. Lanciotti, O. L. Kosoy, J. J. Laven, J. O. Velez, A. J. Lambert, A. J. Johnson, et al. (2008). Emerg. Infect. Dis. 14, 1232.CrossRefGoogle Scholar
  12. 12.
    World Health Organization (2016) WHO statement on the first meeting of the International Health Regulations Emergency Committee on Zika virus and observed increase in neurological disorders and neonatal malformations. 1 February 2016. Geneva: WHO; 2016. http://www.who.int/mediacentre/news/statements/2016/1st-emergency-committee-zika/en/.
  13. 13.
    G. Benelli and H. Mehlhorn (2016). Parasitol. Res. 115, 1747.CrossRefGoogle Scholar
  14. 14.
    M. Govindarajan, H. F. Khater, C. Panneerselvam, and G. Benelli (2016). Res. Vet. Sci. 107, 95.CrossRefGoogle Scholar
  15. 15.
    K. Murugan, P. Aruna, C. Panneerselvam, P. Madhiyazhagan, M. Paulpandi, J. Subramaniam, R. Rajaganesh, H. Wei, M. Saleh Alsalhi, S. Devanesan, M. Nicoletti, B. Syuhei, A. Canale, and G. Benelli (2016). Parasitol. Res. 115, 651.CrossRefGoogle Scholar
  16. 16.
    K. Murugan, C. Panneerselvam, C. M. Samidoss, P. Madhiyazhagan, M. Roni, J. Subramaniam, D. Dinesh, R. Rajaganesh, M. Paulpandi, H. Wei, A. T. Aziz, M. S. Alsalhi, S. Devanesan, M. Nicoletti, R. Pavela, A. Canale, and G. Benelli (2016). Res. Vet. Sci. 106, 14.CrossRefGoogle Scholar
  17. 17.
    K. Murugan, C. Panneerselvam, J. Subramaniam, P. Madhiyazhagan, J. S. Hwang, L. Wang, D. Dinesh, U. Suresh, M. Roni, A. Higuchi, M. Nicoletti, and G. Benelli (2016). Environ. Sci. Pollut. Res. doi: 10.1007/s11356-016-6832-9.Google Scholar
  18. 18.
    G. Benelli (2015). Parasitol. Res. 114, 2801.CrossRefGoogle Scholar
  19. 19.
    G. Benelli (2015). Parasitol. Res. 114, 3201.CrossRefGoogle Scholar
  20. 20.
    D. R. George, R. D. Finn, K. M. Graham, and O. A. E. Sparagano (2014). Parasit. Vect. 7, (28), 1–12.Google Scholar
  21. 21.
    R. Pavela and G. Benelli (2016). Trends Plant Sci. doi: 10.1016/j.tplants.2016.10.005.Google Scholar
  22. 22.
    G. Benelli (2016). Enzyme Microb. Technol. doi: 10.1016/j.enzmictec.2016.08.022.Google Scholar
  23. 23.
    J. K. Patra, S. K. Rath, K. Jena, V. K. Rathod, and H. Thatoi (2008). Turkish J. Biol. 32, 119–125.Google Scholar
  24. 24.
    S. Ravikumar, G. Ramanathan, S. J. Inbaneson, and A. Ramu (2011). Parasitol. Res. 108, 107.CrossRefGoogle Scholar
  25. 25.
    S. Ravikumar, T. Thajudeen, P. Suganthi, S. J. Inbeneson, and T. Vinothkumar (2010). J. Environ. Biol. 31, 387–389.Google Scholar
  26. 26.
    Fortes MD (1990) Seagrasses: a resource unknown in the ASEAN region. ISBN 971-1022-51-6. ICLARM Educ. Ser. (5): pp. 46Google Scholar
  27. 27.
    E. P. Green and F. T. Short (eds.) World Atlas of Seagrasses (University of California Press, Berkeley, 2003).Google Scholar
  28. 28.
    Venkataraman K, Raghunathan C (2015) Coastal and Marine Biodiversity of India. Marine Faunal Diversity in India pp.303–348Google Scholar
  29. 29.
    G. Benelli and M. Govindarajan (2016). J. Clust. Sci. doi: 10.1007/s10876-016-1088-6.Google Scholar
  30. 30.
    A. Ahmad, P. Mukherjee, S. Senapati, D. Mandal, M. I. Khan, R. Kumar, and M. Sastry (2003). Coll. Surf. B 28, 313.CrossRefGoogle Scholar
  31. 31.
    P. Huang, D. P. Yang, C. L. Zhang, J. Lin, M. He, L. Bao, and D. X. Cui (2011). Nanoscale 3, 3623.CrossRefGoogle Scholar
  32. 32.
    B. H. Stuart Polymer Analysis (Wiley, Hoboken, 2002).Google Scholar
  33. 33.
    K. Murugan, G. Benelli, A. Suganya, D. Dinesh, C. Panneerselvam, M. Nicoletti, J. S. Hwang, P. Mahesh Kumar, J. Subramaniam, and U. Suresh (2015). Parasitol. Res. 114, 2243.CrossRefGoogle Scholar
  34. 34.
    K. Murugan, G. Benelli, C. Panneerselvam, J. Subramaniam, T. Jeyalalitha, D. Dinesh, M. Nicoletti, J. S. Hwang, U. Suresh, and P. Madhiyazhagan (2015). Exp. Parasitol. 153, 129–138.CrossRefGoogle Scholar
  35. 35.
    J. B. Cosgrove, R. J. Wood, D. Petric, D. T. Evans, and R. H. Abbott (1994). J. Am. Mosq. Control Assoc. 10, 434.Google Scholar
  36. 36.
    J. A. Mahyoub, A. S. Alsobhi, K. Al-Ghamdi, N. A. Khatter, A. T. Aziz, S. A. Al-Shami, C. Panneerselvam, K. Murugan, M. Nicoletti, A. Canale, and G. Benelli (2016). Asian Pac. J. Trop. Dis. 6, 361.CrossRefGoogle Scholar
  37. 37.
    S. Shanthi, B. D. Jayaseelan, P. Velusamy, S. Vijayakumar, C. T. Chih, and B. Vaseeharan (2016). Microb. Pathog. 93, 70.CrossRefGoogle Scholar
  38. 38.
    D. Dinesh, K. Murugan, P. Madhiyazhagan, C. Panneerselvam, M. Nicoletti, W. Jiang, G. Benelli, B. Chandramohan, and U. Suresh (2015). Parasitol. Res. 114, 1519.CrossRefGoogle Scholar
  39. 39.
    M. Rai, A. Yadav, and A. Gade (2009). Biotechnol. Adv. 27, 76.CrossRefGoogle Scholar
  40. 40.
    D. J. Finney Probit Analysis (Cambridge University, London, 1971), pp. 68–78.Google Scholar
  41. 41.
    N. Basavegowda and Y. R. Lee (2014). J. Nanosci. Nanotechnol. 14, 4377.CrossRefGoogle Scholar
  42. 42.
    P. Mulvaney (1996). Langmuir 12, 788.CrossRefGoogle Scholar
  43. 43.
    M. Roni, K. Murugan, C. Panneerselvam, J. Subramaniam, M. Nicoletti, P. Madhiyazhagan, D. Dinesh, U. Suresh, H. F. Khater, H. Wei, A. Canale, A. A. Alarfaj, M. A. Munusamy, A. Higuchi, and G. Benelli (2015). Ecotoxicol. Environ. Saf. 121, 31.CrossRefGoogle Scholar
  44. 44.
    P. Madhiyazhagan, K. Murugan, A. Naresh Kumar, T. Nataraj, D. Dinesh, C. Panneerselvam, J. Subramaniam, P. Mahesh Kumar, U. Suresh, M. Roni, M. Nicoletti, A. A. Alarfaj, A. Higuchi, M. A. Munusamy, and G. Benelli (2015). Parasitol. Res. doi: 10.1007/s00436-015-4671-0.Google Scholar
  45. 45.
    K. Kalimuthu, C. Panneerselvam, C. Chou, S. M. Lin, L. Tseng, K. H. Tsai, K. Murugan, and J. S. Hwang (2016). Hydrobiologia. doi: 10.1007/s10750-016-2943-z.Google Scholar
  46. 46.
    A. P. de Aragao, T. M. de Oliveira, P. V. Quelemes, M. L. G. Perfeito, M. C. Arau´jo, J. A. S. Santiago, V. S. Cardoso, P. Quaresma, J. R. A. Leite, and D. A. da Silva (2016). Arab. J. Chem. doi: 10.1016/j.arabjc.2016.04.014.Google Scholar
  47. 47.
    G. Sathishkumar, C. Gobinath, K. Karpagam, V. Hemamalini, K. Premkumar, and S. Sivaramakrishnan (2012). Colloid Surf. B 95, 235–240.CrossRefGoogle Scholar
  48. 48.
    K. Murugan, C. M. Samidoss, C. Panneerselvam, A. Higuchi, M. Roni, U. Suresh, B. Chandramohan, J. Subramaniam, P. Madhiyazhagan, D. Dinesh, R. Rajaganesh, A. A. Alarfaj, M. Nicoletti, S. Kumar, H. Wei, A. Canale, H. Mehlhorn, and G. Benelli (2015). Parasitol. Res. 114, 4087.CrossRefGoogle Scholar
  49. 49.
    M. Singh, S. Singh, S. Prasad, and I. S. Gambhir (2008). Dig. J. Nanomater. Biostruct. 3, 115.Google Scholar
  50. 50.
    A. M. Fayaz, K. Balaji, M. Y. R. Girilal, P. T. Kalaichelvan, and R. Venketesan (2010). Nanomed. Nanotechnol. Biol. Med. 6, 103.CrossRefGoogle Scholar
  51. 51.
    S. Kaviya, J. Santhanalakshmi, B. Viswanathan, J. Muthumary, and K. Srinivasan (2011). Spectrochim. Acta, Part A 79, 594.CrossRefGoogle Scholar
  52. 52.
    C. Panneerselvam, K. Murugan, M. Roni, A. T. Aziz, U. Suresh, R. Rajaganesh, P. Madhiyazhagan, J. Subramaniam, D. Dinesh, M. Nicoletti, A. Higuchi, A. A. Alarfaj, M. A. Munusamy, S. Kumar, N. Desneux, and G. Benelli (2016). Parasitol. Res. 115, 997–1013.CrossRefGoogle Scholar
  53. 53.
    P. Anbu, K. Murugan, P. Madhiyazhagan, D. Dinesh, J. Subramaniam, C. Panneerselvam, U. Suresh, A. A. Alarfaj, M. A. Munusamy, A. Higuchi, J. S. Hwang, S. Kumar, M. Nicoletti, and G. Benelli (2015). Nat. Prod. Res. doi: 10.1080/14786419.2015.1114935.Google Scholar
  54. 54.
    G. Suresh, P. H. Gunasekar, D. Kokila, D. Prabhu, D. Dinesh, N. Ravichandran, B. Ramesh, A. Koodalingam, and G. V. Siva (2014). Spectrochim. Acta A 127, 61.CrossRefGoogle Scholar
  55. 55.
    O. Kulikova, D. Mal’tsev, A. Il’ina, A. Burdina, V. Yamskova, and I. Yamskov (2015). Appl. Biochem. Microbiol. 51, 362.CrossRefGoogle Scholar
  56. 56.
    A. Barth (2000). Prog. Biophys. Mol. Biol. 74, 141.CrossRefGoogle Scholar
  57. 57.
    R. Sathyavathi, M. Balamurali Krishna, S. Venugopal Rao, R. Saritha, and D. Narayana Rao (2010). Adv. Sci. Lett. 3, 1.CrossRefGoogle Scholar
  58. 58.
    J. Chowdhury and M. Ghosh (2004). J. Coll. Interf. Sci. 277, 121.CrossRefGoogle Scholar
  59. 59.
    N. Biswas, S. Kapoor, H. S. Mahal, and T. Mukherjee (2007). Chem. Phys. Lett. 444, 338.CrossRefGoogle Scholar
  60. 60.
    P. Dubey, D. Muthukumaran, S. Dash, R. Mukhopadhyay, and S. Sarkar (2005). J. Phys. 65, 681.Google Scholar
  61. 61.
    K. Murugan and D. Jeyabalan (1999). Curr. Sci. 76, 631.Google Scholar
  62. 62.
    K. Murugan, R. Babu, D. Jeyabalan, N. Senthil Kumar, and S. Sivaramakrishnan (1996). J. Entomol. Res. 20, 137.Google Scholar
  63. 63.
    M. Ghaffari-Moghaddam and R. Hadi-Dabanlou (2014). J. Indust. Eng. Chem. 20, 739.CrossRefGoogle Scholar
  64. 64.
    V. Dhand, L. Soumya, S. Bharadwaj, S. Chakra, D. Bhatt, and B. Sreedhar (2016). Mat. Sci. Eng. C 58, 36–43.CrossRefGoogle Scholar
  65. 65.
    S. Rajesh Kumar, M. Paulpandi, M. Manivel Raja, D. Mangalaraj, C. Viswanathan, S. Kannan, and N. Ponpandian (2014). RSC Adv. 4, 13409.CrossRefGoogle Scholar
  66. 66.
    H. Y. Song, K. K. Ko, I. H. Oh, and B. T. Lee (2006). Eur. Cells Mater. 11, 58.Google Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Jazem A. Mahyoub
    • 1
    • 2
  • Al Thabiani Aziz
    • 3
  • Chellasamy Panneerselvam
    • 3
  • Kadarkarai Murugan
    • 4
    • 5
  • Mathath Roni
    • 4
  • Subrata Trivedi
    • 3
  • Marcello Nicoletti
    • 6
  • Usama W. Hawas
    • 7
  • Fekri M. Shaher
    • 7
  • Muneer A. Bamakhrama
    • 7
  • Angelo Canale
    • 8
  • Giovanni Benelli
    • 8
    Email author
  1. 1.Department of Biology Sciences, Faculty of SciencesKing Abdulaziz UniversityJeddahSaudi Arabia
  2. 2.IBB UniversityIbbRepublic of Yemen
  3. 3.Biology Department, Faculty of ScienceUniversity of TabukTabukSaudi Arabia
  4. 4.Division of Entomology, Department of Zoology, School of Life SciencesBharathiar UniversityCoimbatoreIndia
  5. 5.Thiruvalluvar UniversityVelloreIndia
  6. 6.Department of Environmental BiologySapienza University of RomeRomeItaly
  7. 7.Department of Marine Chemistry, Faculty of Marine SciencesKing Abdulaziz UniversityJeddahSaudi Arabia
  8. 8.Department of Agriculture, Food and EnvironmentUniversity of PisaPisaItaly

Personalised recommendations