Journal of Cluster Science

, Volume 28, Issue 1, pp 81–90 | Cite as

Green Synthesis and Characterization of Biologically Active Silver Nanoparticles Using Perilla frutescens Leaf Extract

Original Paper
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Abstract

Silver nanoparticles (AgNPs) were synthesized using an aqueous Perilla leaf extract and evaluated for anticancer activity. These green -synthesized AgNPs were characterized using UV–Visible absorption spectroscopy, XRD, TEM, and SAED. The synthesized AgNPs were spherical and hexagonal in shape, and the average size of the NPs was ~23 nm. SRB assay investigated the anticancer activity of the AgNPs against human cervical cancer (HeLa) cells. The fluorescence and confocal microscopy studies were confirmed the DNA damage and apoptosis following nanoparticle treatment. Furthermore, the intracellular ROS was also increased after treatment. Taking all these data together, it is suggested that AgNPs may be a potential agent for the inhibition of cervical tumor cells.

Keywords

Green synthesis Silver nanoparticles Human cervical cancer cell SRB assay Fluorescence 
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Notes

Acknowledgments

This work was supported by the KU Research Professor Program of Konkuk University, Seoul, South Korea. This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2015R1A2A2A01003741).

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Muthuraman Pandurangan
    • 1
  • P. C. Nagajyothi
    • 2
  • Doo Hwan Kim
    • 1
  • Min-Ji Jung
    • 3
  • Jaesool Shim
    • 2
  • In-Yong Eom
    • 3
    • 4
  1. 1.Department of Bioresources and Food ScienceKonkuk UniversitySeoulSouth Korea
  2. 2.School of Mechanical EngineeringYeungnam UniversityGyeongsan-siSouth Korea
  3. 3.Department of Life ChemistryCatholic University of DaeguGyeongsan-SiSouth Korea
  4. 4.Natural Science Research InstituteCatholic University of DaeguGyeongsan-SiSouth Korea

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