Journal of Cluster Science

, Volume 25, Issue 4, pp 989–1004 | Cite as

Green Synthesis and Characterization of Silver Nanoparticles Using Ascomycota Fungi Penicillium nalgiovense AJ12

  • Irena Maliszewska
  • Anna Juraszek
  • Katarzyna Bielska
Original Paper
First Online:
Received:

Abstract

A new method for green synthesis of silver nanoparticles using the cell-free filtrate of Penicillium nalgiovense AJ12 as reducing and protecting agent was described. The pathway is based on the reduction of Ag1+ by protein(s). Various techniques such as UV–Vis spectroscopy, transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier transform infrared and Zeta potential measurements were used to characterize the silver nanoparticle obtained. The results revealed synthesis of the spherical silver nanoparticles coated with protein(s). The average size of the particles obtained from TEM was 15.2 ± 2.6 nm. DLS measurements showed that the particle size was higher than that estimated from TEM measurements and was 25.2 ± 2.8 nm. Studies on the role of the cell-free filtrate proteins in the synthesis of silver nanoparticles indicated that the process is non-enzymatic but involves amino acids interactions with silver ions. It was found that the aqueous silver nanoparticles suspensions exhibited excellent stability over a wide range of ionic strength, pH and temperature.

Keywords

Fungi Cell-free filtrate Silver nanoparticles Biosynthesis Mechanism 
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Notes

Acknowledgments

This work was supported by NCN (Grant No. NN 507 5150 58) and by a statutory activity subsidy from the Polish Ministry of Science and Higher Education for the Faculty of Chemistry of Wrocław University of Technology.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Irena Maliszewska
    • 1
  • Anna Juraszek
    • 1
  • Katarzyna Bielska
    • 2
  1. 1.Division of Medicinal Chemistry and Microbiology, Faculty of ChemistryWroclaw University of TechnologyWrocławPoland
  2. 2.Department of Biochemistry, Faculty of ChemistryWroclaw University of TechnologyWrocławPoland

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