Journal of Cluster Science

, Volume 22, Issue 2, pp 225–232 | Cite as

Biosynthesis of Silver Nanoparticles by Geotricum sp.

  • Ali Jebali
  • Fatemeh RamezaniEmail author
  • Bahram Kazemi
Original Paper


Nanoparticles are usually 1–100 nm in each spatial dimension considered as building blocks of the next generation of optoelectronics, electronics, and various chemical and biochemical sensors. In the synthesis of nanoparticles use of microorganisms emerges as an eco-friendly and exciting approach that reduce waste products (ultimately leading to atomically precise molecular manufacturing with zero waste); the use of nanomaterials as catalysts for greater efficiency in current manufacturing processes by minimizing or eliminating the use of toxic materials (green chemistry principles); the use of nanomaterials and nanodevices to reduce pollution (e.g. water and air filters); and the use of nanomaterials for more efficient alternative energy production (e.g. solar and fuel cells). Fungi have many advantages for nanoparticle synthesis compared with other organisms. In this study, Geotricum sp. found to successfully produce Ag nanoparticles. Geotricum sp. was grown in SDA (Sabro Dextrose Agar) medium at 25 ± 1 °C for 96 h. The mycelia were used to convert silver nitrate solution into nano-silver. Silver nanoparticles were synthesized using these fungi (Geotricum sp.) extracellularly. UV–VIS spectroscopy, Atomic Force Microscopy (AFM) and Scanning Electron Microscopy images shows the nanoparticle formation in the medium. Energy-dispersive X-ray spectroscopy (EDX) also confirmed that silver nanoparticles in the range of 30–50 nm were synthesized extracellularly. FTIR analyses confirmed the presence of amide (I) and (II) bands of protein as capping and stabilizing agent on the surface of nanoparticles.


Silver Nanoparticle Biosynthesis Geotricum sp. 


  1. 1.
    T. Riddina, M. Gerickeb, and C. G. Whiteleya (2010). EMT J. 46, 501–505.Google Scholar
  2. 2.
    N. Pugazhenthiran and S. Anandan (2009). J. Nanopart. Res. 11, 1811–1815.CrossRefGoogle Scholar
  3. 3.
    M. Rai and A. Yadav, in Applied Mycology (CAB International, New York, 2009).Google Scholar
  4. 4.
    P. Mukherjee and A. Ahmad (2001). Nano Lett. 1, (10), 515–519.CrossRefGoogle Scholar
  5. 5.
    S. Murali (2003). Curr. Sci. 85, 2.Google Scholar
  6. 6.
    M. Karbasian, S. M. Atyabi, and S. D. Siadat (2008). Am. J. Agric. Biol. Sci. 3, (1), 433–437.CrossRefGoogle Scholar
  7. 7.
    K. B. Narayanan and N. Sakthivel (2010). Adv. Colloid Interface Sci. 156, 1–13.CrossRefGoogle Scholar
  8. 8.
    P. Mohanpuria and N. K. Rana (2008). J. Nanopart. Res. 10, 507–517.CrossRefGoogle Scholar
  9. 9.
    N. Saifuddin, C. W. Wong and A. A. Nur (2009). EJ. Chem. 6(1), 61–70.Google Scholar
  10. 10.
    N. Durán, P. D. Marcato, and O. L. Alves (2005). J. Nanobiotechnol. 3, 8.CrossRefGoogle Scholar
  11. 11.
    D. Mandal, M. E. Bolander, and D. Mukhopadhyay (2006). Appl. Microbiol. Biotechnol. 69, 485–492.CrossRefGoogle Scholar
  12. 12.
    L. Sintubin and W. De Windt (2009). Appl. Microbiol. Biotechnol. 84, 741–749.CrossRefGoogle Scholar
  13. 13.
    K. C. Bhainsa and S. F. D’Souza (2006). Colloids Surf. Biointerfaces 47, 160–164.CrossRefGoogle Scholar
  14. 14.
    R. Varshney, A. N. Mishra, S. Bhadauria, and M. S. Gaur (2009). Dig. J. Nanomater. Biostruct. 4, (2), 349–355.Google Scholar
  15. 15.
    A. Nanda and M. Saravanan (2009). Nanomed. Nanotechnol. Biol. Med. 5, 452–456.CrossRefGoogle Scholar
  16. 16.
    K. N. Thakkar, S. S. Mhatre, and R. Y. Parikh (2010). Nanomed. Nanotechnol. Biol. Med. 6, (2), 257–262.CrossRefGoogle Scholar
  17. 17.
    A. K. Jha and K. Prasad (2010). Biotechnol. J. 5, (3), 285–291.CrossRefGoogle Scholar
  18. 18.
    Balaji Dasaratrao Sawle et al (2008). Sci. Technol. Adv. Mater. 9, 035012.Google Scholar
  19. 19.
    A. Ahmad, P. Mukherjee, S. Senapati, D. Mandal, M. I. Khan, R. Kumar, and M. Sastry (2003). Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium semitectum. Colloids Surf. B 28, 313–318.CrossRefGoogle Scholar
  20. 20.
    K. Kalishwaralala, V. Deepaka, and S. B. Ram Kumar Pandian (2010). Colloids Surf. B Biointerfaces 77, 257–262.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Cellular and Molecular Biology Research CenterShahid Beheshti University of Medical SciencesTehranIran

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