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Journal of Clinical Immunology

, Volume 39, Issue 2, pp 216–224 | Cite as

Nasal Nitric Oxide in Primary Immunodeficiency and Primary Ciliary Dyskinesia: Helping to Distinguish Between Clinically Similar Diseases

  • Zofia N. Zysman-Colman
  • Kimberley R. Kaspy
  • Reza Alizadehfar
  • Keith R. NyKamp
  • Maimoona A. Zariwala
  • Michael R. Knowles
  • Donald C. Vinh
  • Adam J. ShapiroEmail author
Original Article
  • 68 Downloads

Abstract

Purpose

Primary ciliary dyskinesia (PCD) is a rare disorder of the mucociliary clearance leading to recurrent upper and lower respiratory tract infections. PCD is difficult to clinically distinguish from other entities leading to recurrent oto-sino-pulmonary infections, including primary immunodeficiency (PID). Nasal nitric oxide (nNO) is a sensitive and specific diagnostic test for PCD, but it has not been thoroughly examined in PID. Past publications have suggested an overlap in nNO levels among subjects with PCD and PID. We sought to determine if nNO measurements among patients diagnosed with PID would fall significantly above the established PCD diagnostic cutoff value of 77 nL/min.

Methods

Children > 5 years old and adults with definitive PID or PCD diagnoses were recruited from outpatient subspecialty clinics. Participants underwent nNO testing by standardized protocol using a chemiluminescence analyzer and completed a questionnaire concerning their chronic oto-sino-pulmonary symptoms, including key clinical criteria specific to diagnosed PCD (neonatal respiratory distress at term birth, year-round cough or nasal congestion starting before 6 months of age, any organ laterality defect).

Results

Participants included 32 patients with PID, 27 patients with PCD, and 19 healthy controls. Median nNO was 228.9.1 nL/min in the PID group, 19.7 nL/min in the PCD group, and 269.4 in the healthy controls (p < 0.0001). Subjects with PCD were significantly more likely to report key clinical criteria specific to PCD, but approximately 25% of PID subjects also reported at least 1 of these key clinical criteria (mainly year-round cough or nasal congestion).

Conclusions

While key clinical criteria associated with PCD often overlap with the symptoms reported in PID, nNO measurement by chemiluminescence technology allows for effective discrimination between PID and PCD.

Keywords

Primary ciliary dyskinesia primary immunodeficiency nasal nitric oxide 

Notes

Acknowledgments

We would like to acknowledge Whitney Wolf (research technician) for specimen and DNA handling and processing and Invitae (San Francisco, California) for performing next-generation sequencing of known PCD genes. We also appreciate Shrikant Mane, Francesc Lopez-Giraldez, and Weilai Dong (Yale Center for Mendelian Genomics, [UM1 HG006504]) for providing whole exome sequencing and bioinformatics support.

Funding

Funds to support this project were received from the Montreal Children’s Hospital Foundation and the National Institute of Health (NIH) research grant nos. U54HL096458 and 5R01HL071798. The Genetic Disorders of Mucociliary Clearance (U54HL096458) is a part of the National Center for Advancing Translational Sciences (NCATS) Rare Disease Clinical Research Network (RDCRN). RDCRN is an initiative of the Office of Rare Diseases Research (ORDR). NCATS funded through a collaboration between NCATS and NHLBI.

Compliance with Ethical Standards

Conflicts of Interest

The authors declare that they have no conflicts of interest.

Ethical Approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Disclaimer

The content of this manuscript are solely the responsibility of the authors and do not necessarily represent the official view of NIH.

Supplementary material

10875_2019_613_MOESM1_ESM.docx (46 kb)
ESM 1 (DOCX 116 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Zofia N. Zysman-Colman
    • 1
  • Kimberley R. Kaspy
    • 2
  • Reza Alizadehfar
    • 2
    • 3
  • Keith R. NyKamp
    • 4
  • Maimoona A. Zariwala
    • 5
  • Michael R. Knowles
    • 6
  • Donald C. Vinh
    • 3
  • Adam J. Shapiro
    • 2
    • 3
    Email author
  1. 1.CHU Sainte-JustineMontrealCanada
  2. 2.Montreal Children’s HospitalMcGill University Health CenterMontrealCanada
  3. 3.McGill University Health Center Research InstituteMontrealCanada
  4. 4.InvitaeSan FransciscoUSA
  5. 5.Department of Pathology and Laboratory Medicine, Marsico Lung InstituteUniversity of North Carolina School of MedicineChapel HillUSA
  6. 6.Department of Medicine, Marsico Lung InstituteUniversity of North Carolina School of MedicineChapel HillUSA

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