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Journal of Clinical Immunology

, Volume 32, Issue 6, pp 1193–1196 | Cite as

Acidomonas Methanolica-Associated Necrotizing Lymphadenitis in a Patient with Chronic Granulomatous Disease

  • John M. ChaseEmail author
  • Steven M. Holland
  • David E. Greenberg
  • Kimberly Marshall-Batty
  • Adrian M. Zelazny
  • Joseph A. ChurchEmail author
Article

Abstract

Purpose

Adenitis for which no causative organism can be isolated is a common occurrence in patients with chronic granulomatous disease (CGD). Here we identify Acidomonas methanolica as a pathogen associated with adenitis in a patient with CGD.

Methods

The causative pathogen was obtained after prolonged incubation of an excised lymph node in thioglycolate broth. Identification was carried out by sequencing the 16s rRNA. Immunoblots were prepared utilizing protein extracts from the case patient’s A. methanolica isolate, an ATCC type strain of A. methanolica and G. bethesdensis.

Results

Fastidious gram-negative rods grew after prolonged incubation of an excised lymph node in thioglycolate broth. Sequencing of the 16s rRNA identified the organism as A. methanolica. Immunoblot confirmed the pathogen’s role in the patient’s adenitis by showing the patient’s specific immune response to the organism.

Conclusions

A. methanolica is the second member of the family, Acetobacteaceae to be associated with adenitis in patients with CGD.

Keywords

Chronic granulomatous disease Acidomonas methanolica 

Notes

Acknowledgments

Funding: Department of Laboratory Medicine, National Institutes of Health

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • John M. Chase
    • 1
    Email author
  • Steven M. Holland
    • 2
  • David E. Greenberg
    • 2
    • 5
  • Kimberly Marshall-Batty
    • 2
    • 5
  • Adrian M. Zelazny
    • 2
    • 3
  • Joseph A. Church
    • 4
    Email author
  1. 1.Division of General Pediatrics, MS#75, Children’s Hospital Los Angeles and Keck School of MedicineUniversity of Southern CaliforniaLos AngelesUSA
  2. 2.Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious DiseasesNational Institutes of HealthBethesdaUSA
  3. 3.Microbiology Service, Department of Laboratory Medicine, Clinical CenterNational Institutes of HealthBethesdaUSA
  4. 4.Division of Clinical Immunology and Allergy, MS#75, Children’s Hospital Los Angeles and Keck School of MedicineUniversity of Southern CaliforniaLos AngelesUSA
  5. 5.Division of Infectious DiseasesUniversity of Texas SouthwesternDallasUSA

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