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Journal of Clinical Immunology

, Volume 32, Issue 6, pp 1270–1278 | Cite as

The Lupus-derived Anti-double-stranded DNA IgG Contributes to Myofibroblast-like Phenotype in Mesangial Cells

  • Yi Zhang
  • Jiabin Yang
  • Shan Jiang
  • Chunhong Fang
  • Layuan Xiong
  • Hong Cheng
  • Yumin XiaEmail author
Article

Abstract

Anti–double-stranded DNA (dsDNA) antibodies have been indicated to play a major role in the pathogenesis of lupus nephritis (LN), which is characterized by mesangial alterations, including phenotypic changes. To explore the effects of anti-dsDNA antibodies on the phenotype of mesangial cells (MCs), the anti-dsDNA IgG in sera and histological features of glomeruli were analyzed in the mice models of immune-complex glomerulonephritis. The MCs were cultured in vitro with the addition of anti-dsDNA or non-anti-dsDNA IgG. Compared to the anti-dsDNA-negative controls, the serum positive mice had increased extracellular matrix accumulation and higher alpha-smooth muscle actin expression in the mesangial region. The anti-dsDNA IgG enhanced the synthesis of transforming growth factor beta, alpha-smooth muscle actin, and fibronectin, and even induced the myofibroblast-like morphological features in cultured MCs. Our results indicated that anti-dsDNA antibodies contribute to the phenotypic changes in MCs, which suggests another mechanism of renal injuries in LN induced by anti-dsDNA antibodies.

Keywords

Autoantibody lupus nephritis mesangial cell myofibroblast phenotype 

Abbreviations

BSA

Bovine serum albumin

dsDNA

Double-stranded DNA

ECM

Extracellular matrix

ELISA

Enzyme-linked immunosorbent assay

HAS

Horse spleen apoferritin

LN

Lupus nephritis

MC

Mesangial cell

PBS

Phosphate-buffered saline

PCR

Polymerase chain reaction

SLE

Systemic lupus erythematosus

SMA

Smooth muscle actin

TGF

Transforming growth factor

Notes

Acknowledgements

This work was supported by a grant from National Natural Science Foundation of China (Project No.30972640, to CF and YX).

Conflict of interest statement

The authors declare that there are no competing interests.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Yi Zhang
    • 1
    • 2
  • Jiabin Yang
    • 3
  • Shan Jiang
    • 4
  • Chunhong Fang
    • 4
  • Layuan Xiong
    • 1
  • Hong Cheng
    • 1
  • Yumin Xia
    • 4
    Email author
  1. 1.Department of MedicineRenmin Hospital of Wuhan UniversityWuhanChina
  2. 2.Department of Medicine, Gezhouba Central HospitalThe Third Clinical Hospital of Three Gorges UniversityYichangChina
  3. 3.Department of MedicineThe First Affiliated Hospital of Yangtze UniversityJingzhouChina
  4. 4.Department of DermatologyRenmin Hospital of Wuhan UniversityWuhanChina

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