Journal of Clinical Immunology

, Volume 32, Issue 3, pp 505–513

Bmi-1 Regulates Autoreactive CD4+ T Cell Survival in Immune Thrombocytopenia Patients

  • Li Ma
  • Zeping Zhou
  • Donglei Zhang
  • Hongmei Wang
  • Huiyuan Li
  • Feng Xue
  • Renchi Yang
Article

DOI: 10.1007/s10875-011-9643-2

Cite this article as:
Ma, L., Zhou, Z., Zhang, D. et al. J Clin Immunol (2012) 32: 505. doi:10.1007/s10875-011-9643-2

Abstract

Autoreactive T cells in immune thrombocytopenia (ITP) patients undergo a rapid clonal expansion and are resistant to apoptosis to maintain continuous effect in thrombocytopenia. As Bmi-1 is involved in memory CD4+ T cell survival and Th2 proliferation, we hypothesized that Bmi-1 may have a role in autoreactive CD4+ T cell clonal expansion and Th1/Th2 development in ITP patients. We found that CD4+ T cells from active ITP patients had a higher Bmi-1 expression in comparison with remission and healthy controls, and autoreactive CD4+ T cells had more capability to proliferate and resistance to apoptosis than that of healthy controls. We evaluated the part that Bmi-1 played in proliferation and Th1 bias condition of autoreactive CD4+ T cells in ITP. We used lentiviral transfer vectors containing Bmi-1 and shBmi-1 to infect CD4+ T cells from ITP patients and healthy controls during autologous platelets stimulation. Flow cytometry and ELISA were applied to detect various parameters. The results showed that suppression of Bmi-1 using short hairpin RNA inhibited the platelet-mediated proliferation and increased apoptosis of autoreactive CD4+ T cells from ITP patients. Increased Bmi-1 expression in CD4+ T cells from healthy controls promoted the proliferation and inhibited apoptosis of CD4+ T cells. Bmi-1 significantly promoted interleukin-4 secretion by CD4+ T cells. These findings suggest that Bmi-1 plays a part in autoreactive CD4+ T cell proliferative capability and apoptotic resistance in ITP patients.

Keywords

ITP Bmi-1 CD4+ T cells platelets proliferation 

Supplementary material

10875_2011_9643_MOESM1_ESM.doc (1.5 mb)
ESM 1(DOC 1523 kb)

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Li Ma
    • 1
  • Zeping Zhou
    • 1
    • 2
  • Donglei Zhang
    • 1
  • Hongmei Wang
    • 1
  • Huiyuan Li
    • 1
  • Feng Xue
    • 1
  • Renchi Yang
    • 1
  1. 1.State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeTianjinPeople’s Republic of China
  2. 2.Department of HematologySecond Affiliated Hospital of Kunming Medical UniversityKunmingPeople’s Republic of China

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