Determination of Human Anticytokine Autoantibody Profiles Using a Particle-Based Approach
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Anticytokine autoantibodies cause numerous human diseases, ranging from pure red cell aplasia to acquired immunodeficiencies. Rapid, simple, and affordable detection and monitoring of these antibodies is essential. We sought to develop a standardizable assay that is rapid, sensitive, and specific and able to simultaneously detect multiple anticytokine autoantibodies in small volumes (<10 μl).
We conjugated purified human cytokines to commercially available fluorescently labeled microspheres and tested them against sera from well-characterized subjects with at least one high-titer, disease-associated anticytokine autoantibody.
Cytokine-conjugated microspheres efficiently and rapidly determined plasma concentration and IgG subclass of anticytokine autoantibodies in single or multiplex formats.
This particle-based multiplex assay can reproducibly characterize anticytokine autoantibodies. This efficient and inexpensive approach to diagnosing and monitoring anticytokine autoantibodies has clinical applications.
KeywordsInterferon gamma luminex thymoma pulmonary alveolar proteinosis immunodeficiency
This work was supported by the Division of Intramural Research at the National Institute of Allergy and Infectious Diseases and Colgate University.
- 4.Kavuru MS, Malur A, Marshall I, Barna BP, Meziane M, Huizar I, et al. An open-label trial of rituximab therapy in pulmonary alveolar proteinosis. Eur Respir J. 2011. doi:10.1183/09031936.00197710.
- 14.Sun M, Manolopoulou J, Spyroglou A, Beuschlein F, Hantel C, Wu Z, et al. A microsphere-based duplex competitive immunoassay for the simultaneous measurements of aldosterone and testosterone in small sample volumes: validation in human and mouse plasma. Steroids. 2010;75(13–14):1089–96.PubMedCrossRefGoogle Scholar