Journal of Clinical Immunology

, Volume 32, Issue 1, pp 114–123

Cognate Th2–B Cell Interaction is Essential for the Autoantibody Production in Pemphigus Vulgaris

  • Haiqin Zhu
  • Yayuan Chen
  • Yun Zhou
  • Ying Wang
  • Jie Zheng
  • Meng Pan
Article

DOI: 10.1007/s10875-011-9597-4

Cite this article as:
Zhu, H., Chen, Y., Zhou, Y. et al. J Clin Immunol (2012) 32: 114. doi:10.1007/s10875-011-9597-4

Abstract

Pemphigus vulgaris (PV) is a Th2-dominant autoimmune skin disease. We showed that indeed active PV patients had a biased Th2 response and specific IgG4 autoantibodies were dominant. To further investigate the role of antigen-specific Th2 cells in the regulation of pathogenic Dsg3-IgG antibody production, we used recombined Dsg3 protein to immunize wild-type C57BL/6 mice with aluminum hydroxide or complete Freund’s adjuvant as adjuvant. CD4+ T cells from Dsg3-immunized mice were adoptively transferred into TCR-β chain deficient mice. The transferred CD4+ T cells were readily seen in the peripheral blood and spleen, and interacted with B cells, resulting in B-cell activation. Furthermore, transferred CD4+ T cells from mice immunized with Dsg3 plus Alum with Th2 phenotype were able to render unprimed B cells to secrete Dsg3-specific IgG1 antibody in vivo. Taken together, these results provide the first demonstration of direct role of Dsg3-reactive CD4+ T (Th2) cells in the regulation of pathologic anti-Dsg3 antibody production.

Keywords

Pemphigus vulgaris Dsg3 Th2 cell IgG4 subclass 

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Haiqin Zhu
    • 1
  • Yayuan Chen
    • 1
  • Yun Zhou
    • 2
  • Ying Wang
    • 2
  • Jie Zheng
    • 1
  • Meng Pan
    • 1
  1. 1.Department of Dermatology, Ruijin Hospital, School of MedicineShanghai Jiao Tong UniversityShanghaiChina
  2. 2.Shanghai Institute of Immunology, School of MedicineShanghai Jiaotong UniversityShanghaiChina

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