Journal of Clinical Immunology

, Volume 31, Issue 4, pp 540–549 | Cite as

Thymic and Bone Marrow Output in Patients with Common Variable Immunodeficiency

  • Federico Serana
  • Paolo Airò
  • Marco Chiarini
  • Cinzia Zanotti
  • Mirko Scarsi
  • Micol Frassi
  • Vassilios Lougaris
  • Alessandro Plebani
  • Luigi Caimi
  • Luisa Imberti
Article

Abstract

Objective

The study aims to obtain more information about the immune deficit of common variable immunodeficiency (CVID) patients.

Materials and Methods

A new real-time PCR assay was used to quantify T and B lymphocyte mobilization from the production and maturation sites through the detection of T cell receptor excision circles (TRECs) and kappa-deleting recombination circles (KRECs) and to allow the estimation of the average number of B cell divisions. T and B lymphocyte subsets were analyzed by flow cytometry.

Results

The number of TREC+ lymphocytes, which depends on age and gender, was significantly reduced in CVID patients. Similarly, KREC concentration was lower than in controls. Classification of patients according to the percentage of memory switched B cells showed that patients belonging to MB2 group and therefore with conserved B cell maturation have the lowest new B cell output but increased average peripheral divisions, leading to the highest B cell number.

Conclusions

TREC and KREC quantification can be helpful for a more complete and informative understanding of a heterogeneous disease such as CVID.

Keywords

Common variable immunodeficiency kappa-deleting recombination circles T cell receptor excision circles 

Supplementary material

10875_2011_9526_MOESM1_ESM.ppt (70 kb)
Supplementary Figure 1Correlation between the average number of divisions and KRECs per milliliter in CVID patients. The number of KRECs per milliliter of each patient is plotted against the respective average number of B cell divisions. The line was obtained by simple linear regression calculated after log transformation of KRECs per milliliter values. (PPT 70 kb)
10875_2011_9526_MOESM2_ESM.xls (30 kb)
Supplementary Table 1Percentage and number of T and B lymphocyte subpopulations (XLS 30 kb)

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Federico Serana
    • 1
  • Paolo Airò
    • 3
  • Marco Chiarini
    • 2
  • Cinzia Zanotti
    • 2
  • Mirko Scarsi
    • 3
  • Micol Frassi
    • 3
  • Vassilios Lougaris
    • 4
  • Alessandro Plebani
    • 4
  • Luigi Caimi
    • 2
  • Luisa Imberti
    • 2
  1. 1.Department of Biomedical Science and BiotechnologyUniversity of BresciaBresciaItaly
  2. 2.Laboratorio di Biotecnologie, Diagnostics DepartmentSpedali Civili di BresciaBresciaItaly
  3. 3.Rheumatology and Clinical Immunology ServiceSpedali Civili and University of BresciaBresciaItaly
  4. 4.Pediatrics and Laboratory of Molecular Medicine “A. Nocivelli”Spedali Civili di BresciaBresciaItaly

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