Thymic and Bone Marrow Output in Patients with Common Variable Immunodeficiency
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The study aims to obtain more information about the immune deficit of common variable immunodeficiency (CVID) patients.
Materials and Methods
A new real-time PCR assay was used to quantify T and B lymphocyte mobilization from the production and maturation sites through the detection of T cell receptor excision circles (TRECs) and kappa-deleting recombination circles (KRECs) and to allow the estimation of the average number of B cell divisions. T and B lymphocyte subsets were analyzed by flow cytometry.
The number of TREC+ lymphocytes, which depends on age and gender, was significantly reduced in CVID patients. Similarly, KREC concentration was lower than in controls. Classification of patients according to the percentage of memory switched B cells showed that patients belonging to MB2 group and therefore with conserved B cell maturation have the lowest new B cell output but increased average peripheral divisions, leading to the highest B cell number.
TREC and KREC quantification can be helpful for a more complete and informative understanding of a heterogeneous disease such as CVID.
KeywordsCommon variable immunodeficiency kappa-deleting recombination circles T cell receptor excision circles
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