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Journal of Clinical Immunology

, Volume 31, Issue 1, pp 74–79 | Cite as

Galactosylation of Serum IgA1 O-Glycans in Celiac Disease

  • Katri LindforsEmail author
  • Hitoshi Suzuki
  • Jan Novak
  • Pekka Collin
  • Päivi Saavalainen
  • Lotta L. E. Koskinen
  • Markku Mäki
  • Katri Kaukinen
Article

Abstract

In celiac disease, gluten ingestion provokes small-bowel mucosal injury and production of IgA autoantibodies against transglutaminase 2 (TG2). It has been suggested that in celiac patients IgA could mediate the transepithelial passage of gluten peptides in a mechanism involving the transferrin receptor. As IgA1 with galactose-deficient O-linked glycans has elevated affinity for the transferrin receptor, we assessed whether total serum IgA1 and IgA1 anti-TG2 autoantibodies in celiac patients are aberrantly glycosylated. We report that males with celiac disease have higher total serum levels of galactose-deficient IgA1 than non-celiac males. Furthermore, O-glycans of the disease-specific TG2 IgA1 autoantibodies in celiac patients exhibited elevated galactose deficiency. A gluten-free diet had no effect on the total serum levels of galactose-deficient IgA1, whereas the amount of galactose-deficient anti-TG2 IgA1 decreased. Thus, the undergalactosylated IgA1 molecules are not pathognomonic for celiac disease, but galactose deficiency in IgA1 could be an aggravating factor.

Keywords

Celiac disease gluten IgA1 galactose deficiency anti-transglutaminase 2-autoantibody 

Notes

Acknowledgements

The Celiac Disease Study Group has been financially supported by the Academy of Finland, the Sigrid Juselius Foundation, the Pediatric Research Foundation, the Competitive Research Funding of Tampere University Hospital, the Research Fund of the Finnish Celiac Society and the European Commission (contract number MRTN-CT-2006-036032). HS and JN were supported in part by grants from the National Institute of Health DK078244, DK080301, DK082753, DK083663, DK071802, and DK077279.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Katri Lindfors
    • 1
    • 6
    Email author
  • Hitoshi Suzuki
    • 2
    • 3
  • Jan Novak
    • 2
  • Pekka Collin
    • 4
  • Päivi Saavalainen
    • 5
  • Lotta L. E. Koskinen
    • 5
  • Markku Mäki
    • 1
  • Katri Kaukinen
    • 4
    • 6
  1. 1.Pediatric Research CenterUniversity of Tampere and Tampere University HospitalTampereFinland
  2. 2.Department of MicrobiologyUniversity of Alabama at BirminghamAlabamaUSA
  3. 3.Department of NephrologyJuntendo University Faculty of MedicineTokyoJapan
  4. 4.Department of Gastroenterology and Alimentary Tract SurgeryTampere University HospitalTampereFinland
  5. 5.Department of Medical Genetics and Research Program for Molecular Medicine, Biomedicum HelsinkiUniversity of HelsinkiHelsinkiFinland
  6. 6.Medical SchoolUniversity of TampereTampereFinland

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