Journal of Clinical Immunology

, Volume 31, Issue 1, pp 122–127

Dexamethasone-Induced FKBP51 Expression in Peripheral Blood Mononuclear Cells Could Play a Role in Predicting the Response of Asthmatics to Treatment with Corticosteroids

  • Eunyoung Chun
  • Hyun-Seung Lee
  • Bo-Ram Bang
  • Tae-Wan Kim
  • So-Hee Lee
  • Ju-Han Kim
  • Sang-Heon Cho
  • Kyung-Up Min
  • You-Young Kim
  • Heung-Woo Park
Article

Abstract

Background

Corticosteroids (CSs) are the preferred anti-inflammatory therapy for the treatment of asthma, but the responses of asthmatics to CSs are known to vary. It has thus become important to discover reliable markers in predicting responses to CSs.

Methods

We performed time-series microarrays using a murine model of asthma after a single dose of dexamethasone, based on the assumption that the gene showing a greater change in response to CSs can also be a potential marker for that finding. We then evaluated the clinical meaning of the gene discovered in the microarray experiments.

Results

We found that the expression of FK506 binding protein 51 gene (FKBP51) in lung tissue markedly increased after dexamethasone treatment in a murine model of asthma. We then measured dexamethasone-induced FKBP51 expression in peripheral blood mononuclear cells (PBMCs) in asthmatics. Dexamethasone-induced FKBP51 expression in PBMCs was significantly higher in severe asthmatics compared with mild-to-moderate asthmatics treated with inhaled CSs. In addition, we found that dexamethasone-induced FKBP51 expression in PBMCs was inversely correlated with improvement in lung function after treatment with orally administered prednisolone in six steroid-naive asthmatics.

Conclusion

Dexamethasone-induced FKBP51 expression in PBMCs may be a reliable and practical biomarker in predicting the response to CSs in asthmatics.

Keywords

Asthma glucocorticoids peripheral blood mononuclear cells FKBP51 

Abbreviations

CSs

Corticosteroids

FKBP51

FK506 binding protein 51

GR

Glucocorticoid receptor

PBMCs

Peripheral blood mononuclear cells

PBS

Phosphate-buffered saline

Supplementary material

10875_2010_9463_MOESM1_ESM.pdf (206 kb)
ESM 1(PDF 205 kb)

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Eunyoung Chun
    • 1
    • 2
  • Hyun-Seung Lee
    • 1
    • 2
  • Bo-Ram Bang
    • 1
    • 2
  • Tae-Wan Kim
    • 1
    • 2
  • So-Hee Lee
    • 1
    • 2
  • Ju-Han Kim
    • 3
  • Sang-Heon Cho
    • 1
    • 2
  • Kyung-Up Min
    • 1
    • 2
  • You-Young Kim
    • 1
    • 2
  • Heung-Woo Park
    • 1
    • 2
  1. 1.Department of Internal MedicineSeoul National University College of MedicineSeoulRepublic of Korea
  2. 2.Institute of Allergy and Clinical ImmunologySeoul National University College of MedicineSeoulRepublic of Korea
  3. 3.Seoul National University Biomedical InformaticsSeoul National University College of MedicineSeoulRepublic of Korea

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