Reduced CD27 Expression on Antigen-Specific CD4+ T Cells Correlates with Persistent Active Tuberculosis
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CD27, a member of the tumor necrosis factor receptor family, has important role in generation of T cell immunity. In this study, association of CD27 expression on mycobacterial antigen-specific CD4+ T cells with pulmonary tuberculosis (TB) was investigated.
Mycobacterial antigen-specific CD4+ T cells were identified based on CD154 expression and CD27 expression on antigen-specific CD4 T cells was analyzed by flow cytometry.
Compared with tuberculin-positive controls, patients with bacterial culture-positive pulmonary TB had significantly reduced CD27 expression on antigen-specific CD4 T cells. The persistent active TB patients had much lower percentages of CD27+ antigen-specific CD4 T cells than culture-positive new TB patients (P = 0.008) and healthy controls (P = 0.005). Logistic regression analysis on frequencies of CD27-expressing antigen-specific CD4 T cells and TB patients’ clinical characteristics indicated that low percentage of CD27+ antigen-specific CD4 T cells correlated significantly with persistent active tuberculosis (P = 0.002, odds ratio = 19.6).
It is concluded that frequency of CD27+ antigen-specific CD4 T cells could be used as an immunological marker for persistent active TB.
KeywordsCD4+ T cells CD27 tuberculosis biomarker persistent infection
The study was supported by a grant for infectious diseases from Ministry of Health, China to XC (2008ZX10003-012), a grant from the Beijing Natural Science Foundation, China (7092100), and a grant from the Natural Science Foundation of China (30470094). The work described in the study was performed at the Division of Research, Institute of Tuberculosis, 309 Hospital, Beijing, China.
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