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Journal of Clinical Immunology

, Volume 30, Issue 3, pp 426–434 | Cite as

Proteolytically Inactive Per a 10 Allergen of Periplaneta americana Modulates Th2 Response and Enhances IL-10 in Mouse Model

  • Deepsikha Srivastava
  • Amit Kumar Mehta
  • Naveen Arora
  • Shailendra Nath Gaur
  • Bhanu Pratap SinghEmail author
Article

Abstract

Background

Purified allergens with reduced IgE reactivity are required to improve the safety and efficacy of allergen-specific immunotherapy (IT).

Objective

The present study investigates the efficacy of purified cockroach allergen immunotherapy with proteolytically active and inactive Per a 10 in allergic mouse model.

Methods

Balb/c mice were sensitized intraperitoneally with cockroach extract (CE) and purified allergen Per a 10 in separate groups. Mice were treated subcutaneously with phosphate-buffered saline (PBS), CE, active and inactive Per a 10 and challenged intranasally. Antigen specific IgE, IgG1 and IgG2a in serum and cytokines IL-4, IL-13, IFN-γ, IL-10, TGF-β in bronchoalveolar lavage (BAL) fluid and spleen culture supernatant (CS) were estimated by enzyme-linked immunosorbent assay. Lung histology was analyzed by hematoxylin and eosin staining.

Results

IT with Per a 10 demonstrated significant reduction in IgE levels in serum, IL-4 levels in BAL fluid, CS, and eosinophilic infiltration in lungs than PBS-treated mice. This was associated with significantly increased IL-10 secretion in BAL fluid and CS. IT with Per a 10 effectively suppressed T-helper type 2 (Th2) response in mice sensitized with Per a 10 than CE group. Further, IT with inactive Per a 10 showed maximum reduction in systemic and airway inflammation and induced maximum IL-10 release in BAL fluid and CS than other antigens.

Conclusions

IT with Per a 10 effectively suppressed Th2 response and lung inflammation in Per a 10- or CE-sensitized mice. The beneficial effects of IT with inactive Per a 10 are more pronounced than active Per a 10.

Keywords

Animal model allergen immunotherapy P. americana Per a 10 serine protease 

Abbreviations

SIT

Specific Immunotherapy

CS

Spleen culture supernatant

EDTA

Ethylenediamine tetraceticacid

AEBSF

Aminoethyl benzenesulfonyl fluoride hydrochloride

PMSF

Phenylmethylsulfonyl fluoride

BAEE

N-Benzoyl arginine ethyl ester hydrochloride

CE

Whole body cockroach extract

w/v

Weight/volume

SDS

Sodium dodecyl sulfate

PBS

Phosphate-buffered saline

ELISA

Enzyme-linked immunosorbent assay

BAL

Bronchoalveolar lavage

Notes

Acknowledgment

One of the authors (Deepsikha) received financial assistance from Council of Scientific and Industrial Research, New Delhi, India.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Deepsikha Srivastava
    • 1
    • 2
  • Amit Kumar Mehta
    • 1
    • 2
  • Naveen Arora
    • 1
  • Shailendra Nath Gaur
    • 3
  • Bhanu Pratap Singh
    • 1
    Email author
  1. 1.Allergy and Immunology Section, Institute of Genomics and Integrative Biology (CSIR)Delhi University CampusDelhiIndia
  2. 2.Department of BiotechnologyUniversity of PunePuneIndia
  3. 3.Department of Pulmonary Medicine, V. P. Chest InstituteUniversity of DelhiDelhiIndia

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