Journal of Clinical Immunology

, Volume 29, Issue 5, pp 603–610

High-Dose Dexamethasone Inhibits BAFF Expression in Patients with Immune Thrombocytopenia

  • Xiao-juan Zhu
  • Yan Shi
  • Jian-zhi Sun
  • Ning-ning Shan
  • Jun Peng
  • Cheng-shan Guo
  • Ping Qin
  • Ming Hou
Article

DOI: 10.1007/s10875-009-9303-y

Cite this article as:
Zhu, X., Shi, Y., Sun, J. et al. J Clin Immunol (2009) 29: 603. doi:10.1007/s10875-009-9303-y

Abstract

Introduction

B-cell activating factor belonging to the TNF family (BAFF) is elevated in several autoimmune diseases including immune thrombocytopenia (ITP). High-dose dexamethasone (HD-DXM) has shown its clinical efficacy in ITP patients.

Materials and Methods

The plasma BAFF concentration and BAFF mRNA were measured in ITP patients before and after oral administration of 40 mg/day DXM for four consecutive days by enzyme-linked immunosorbent assay (ELISA) and real-time quantitative PCR. Moreover, we evaluated the effects of DXM on BAFF expression and proliferation of lymphocytes by ELISA, real-time quantitative PCR and cell proliferation respectively in in vitro experiment.

Results

Both plasma BAFF concentration and BAFF mRNA were significantly increased in active ITP patients at pretherapy when compared with controls (P < 0.001). After 4-day treatment with HD-DXM, the BAFF and BAFF mRNA were decreased, and lower than that for controls. In in vitro assays, we found DXM-inhibited BAFF, IFN-γ expression, and the proliferation of lymphocytes in a dose-dependent manner.

Conclusion

These results suggest that BAFF expression is increased in ITP patients with active disease, and DXM is an effective inhibitor of BAFF production. As immunosuppressant, DXM may play its role in ITP treatment partly through regulating BAFF expression.

Keywords

B-cell activating factor belonging to the TNF family dexamethasone immune thrombocytopenia 

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Xiao-juan Zhu
    • 1
  • Yan Shi
    • 1
    • 5
  • Jian-zhi Sun
    • 1
  • Ning-ning Shan
    • 1
    • 2
  • Jun Peng
    • 1
    • 3
  • Cheng-shan Guo
    • 4
  • Ping Qin
    • 1
  • Ming Hou
    • 1
    • 3
  1. 1.Haematology Oncology Centre, Qilu HospitalShandong UniversityJinanChina
  2. 2.Department of Clinical Laboratory, Qilu HospitalShandong UniversityJinanChina
  3. 3.The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, Qilu HospitalShandong UniversityJinanChina
  4. 4.Department of HematologySecond Hospital of Shandong UniversityJinanChina
  5. 5.Department of Hematology, Qilu HospitalShandong UniversityJinanChina

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