An Association Study of 13 SNPs from Seven Candidate Genes with Pediatric Asthma and a Preliminary Study for Genetic Testing by Multiple Variants in Taiwanese Population
- 256 Downloads
Asthma is one of the most common chronic diseases in children. It is caused by complex interactions between various genetic factors and exposures to environmental allergens and irritants. Because of the heterogeneity of the disease and the genetic and cultural differences among different populations, a proper association study and genetic testing for asthma and susceptibility genes is difficult to perform.
Materials and Methods
We assessed 13 single-nucleotide polymorphisms (SNPs) in seven well-known asthma susceptibility genes and looked for association with pediatric asthma using 449 asthmatic subjects and 512 non-asthma subjects in Taiwanese population.
CD14-159 C/T and MS4A2 Glu237Gly were identified to have difference in genotype/allele frequencies between the control group and asthma patients. Moreover, the genotype synergistic analysis showed that the co-contribution of two functional SNPs was riskier or more protective from asthma attack. Our study provided a genotype synergistic method for studying gene–gene interaction on polymorphism basis and genetic testing using multiple polymorphisms.
KeywordsPediatric asthma SNPs genetic testing asthma susceptibility genes
- 12.Burney PG, Luczynska C, Chinn S, Jarvis D. The European Community Respiratory Health Survey. Eur Respir J. 1994;5:954–60.Google Scholar
- 13.Pearce N, Sunyer J, Cheng S, Chinn S, Bjorksten B, Burr M, Keil U, Anderson HR, Burney P. Comparison of asthma prevalence in the ISAAC and the ECRHS. ISAAC Steering Committee and the European Community Respiratory Health Survey. International Study of Asthma and Allergies in Childhood. Eur Respir J. 2000;16:420–6. doi: 10.1183/9031936.00.16337700.PubMedCrossRefGoogle Scholar
- 14.Beydon N, Davis SD, Lombardi E, Allen JL, Arets HGM, Aurora P, Bisgaard H, Davis GM, Ducharme FM, Eigen H, Gappa M, Gaultier C, Gustafsson PM, Hall GL, Hantos Z, Healy MJR, Jones MH, Klug B, Carlsen KCL, McKenzie SA, Marchal F, Mayer OH, Merkus PJFM, Morris MG, Oostveen E, Pillow JJ, Seddon PC, Silverman M, Sly PD, Stocks J, Tepper RS, Vilozni D, Wilson NH, behalf of the American Thoracic Society/European Respiratory Society Working Group on Infant and Young Children Pulmonary Function Testing. An official American Thoracic Society/European Respiratory Society Statement: pulmonary function testing in preschool children. Am J Respir Crit Care Med. 2007;175:1304–45. doi: 10.1164/rccm.200605-642ST.PubMedCrossRefGoogle Scholar
- 17.Unoki M, Furuta S, Onouchi Y, Watanabe O, Doi S, Fujiwara H, Miyatake A, Fujita K, Tamari M, Nakamura Y. Association studies of 33 single nucleotide polymorphisms (SNPs) in 29 candidate genes for bronchial asthma: positive association a T294C polymorphism in the thromboxane A2 receptor gene. Hum Genet. 2000;106:440–6. doi: 10.1007/s004390000267.PubMedCrossRefGoogle Scholar
- 18.Harkonarson H, Bjornsdottir US, Ostermann E, Arnason T, Adalsteinsdottir AE, Halapi E, Shkolny D, Kristjansson K, Gidnadottir SA, Frigge ML, Gislason D, Gislason T, Kong A, Gulcher J, Stefansson K. Allelic frequencies and patterns of single-nucleotide polymorphisms in candidate genes for asthma and atopy in Iceland. Am J Respir Crit Care Med. 2001;164:2036–44.Google Scholar
- 25.Wu JY, Shyur SD, Huang LH, Weng JD, Chuo HT, Ma YC, Lin SC, Wu WC. Symptom scores, pulmonary unction tests and levels of allergen specific IgE in asthmatic children. The Official Bulletin of the Society of Pediatric Pulmonology, Taiwan. ROC. 2003;4:1–7.Google Scholar
- 31.Liu X, Beaty TH, Deindl P, Huang SK, Lau S, Sommerfeld C, Fallin MD, Kao WH, Wahn U, Nickel R. Associations between specific serum IgE response and 6 variants within the genes IL4, IL13, and IL4RA in German children: the German Multicenter Atopy Study. J Allergy Clin Immunol. 2004;113:489–95. doi: 10.1016/j.jaci.2003.12.037.PubMedCrossRefGoogle Scholar