Journal of Clinical Immunology

, Volume 29, Issue 1, pp 90–98 | Cite as

Downregulation of Inflammatory Responses by Novel Caffeic Acid Ester Derivative by Inhibiting NF-kappa B

  • Julie S. Bose
  • Vijay Gangan
  • Swatantra Kumar Jain
  • Sunil K. Manna



Considering anti-tumorigenic activity of caffeic acid phenyl ester, synthesis of several esterified form of caffeic acid is a novel approach in designing for potent drugs.


Our study demonstrates that esterified caffeic acid with methyl vanillate, termed as caffeic acid methyl vanillate ester (CAMVE), blocked inflammatory stimuli-induced inflammatory responses. It decreased amounts of iNOS, Cox-2, and ICAM1 by inhibiting NF-κB through inhibition of IKK activity, IκBα degradation, and p65 nuclear translocation.


Overall, our data suggest that novel caffeic acid ester down-regulates inflammatory responses through inhibition of NF-κB and dependent several gene expressions, further suggesting its efficacy as a promising therapeutic agent.


CAMVE NF-κB inflammation Cox-2 ROI TNF 



methyl 4-[{(2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl}oxy]-3-methoxybenzoate and/or caffeic acid methyl vanillate ester




fluorescein isothiocyanate


intercellular adhesion molecules


inhibitory subunit of NF-kappaB


IκBα kinase




mean fluorescence intensity


nuclear transcription factor kappa B




nuclear extract


reactive oxygen intermediate


secretory alkaline phosphatase


thiobarbituric-acid reactive substances


tumor necrosis factor



This work was supported by the Reliance Life Sciences and core grant of Centre for DNA Fingerprinting and Diagnostics (CDFD). We duly thank Dr. J. V. Raman, Research Director and Head, Chemical Research Technology Group, Reliance Life Sciences for valuable comments on the work.


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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Julie S. Bose
    • 1
  • Vijay Gangan
    • 1
  • Swatantra Kumar Jain
    • 2
  • Sunil K. Manna
    • 3
  1. 1.Reliance Life Sciences Pvt. Ltd.MumbaiIndia
  2. 2.Department of BiotechnologyJamia HamdardNew DelhiIndia
  3. 3.Laboratory of ImmunologyCentre for DNA Fingerprinting and Diagnostics (CDFD)HyderabadIndia

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