Journal of Clinical Immunology

, Volume 29, Issue 1, pp 46–56 | Cite as

Roles of Reactive Oxygen Species in CXCL8 and CCL2 Expression in Response to the 30-kDa Antigen of Mycobacterium tuberculosis

  • Hye-Mi Lee
  • Dong-Min Shin
  • Kwang-Kyu Kim
  • Ji-Sook Lee
  • Tae-Hyun Paik
  • Eun-Kyeong Jo



The 30-kDa antigen (Ag) of Mycobacterium tuberculosis (M. tuberculosis) is a strong inducer of innate and adaptive immune responses in human tuberculosis. The generation of reactive oxygen species (ROS) plays an important role in inflammatory signaling as well as antimicrobial defense.

Materials and Methods

In this study, we investigated the role of ROS in the activation of mitogen-activated protein kinases (MAPKs) and secretion of the CXC chemokine ligand 8 (CXCL8) and CC chemokine ligand 2 (CCL2) by human monocytes stimulated with the 30-kDa Ag of M. tuberculosis H37Rv.


Treatment of human monocytes with the 30-kDa Ag activated rapid superoxide generation. In addition, the 30-kDa Ag activated mRNA and protein expression of CXCL8 and CCL2 in human primary monocytes through nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-dependent ROS generation. Analysis of MAPK activation (extracellular signal-regulated kinase (ERK) 1/2 and p38) showed rapid phosphorylation of both subfamilies in response to the 30-kDa Ag. In addition, 30-kDa-induced MAPK activation was inhibited in a dose-dependent manner by pretreatment with ROS scavengers. Toll-like receptor (TLR) 2 was required for ROS generation, chemokine production, and MAPK activation following stimulation with the 30-kDa Ag. Using highly specific signaling pathway inhibitors, we found that both p38 and ERK1/2 activation are essential for 30-kDa Ag-induced CCL2 but not CXCL8 production in human monocytes.


These results indicate that TLR2–ROS signaling plays a crucial role in the 30-kDa Ag-mediated expression of CXCL8 and CCL2 in human monocytes. In addition, both p38 and ERK1/2 activation are essential for 30-kDa Ag-stimulated CCL2 production by monocytes.


Mycobacteria 30-kDa antigen ROS MAPK CXCL8 CCL2 



This work was supported by a grant of the Korea Science and Engineering Foundation through the Infection Signaling Network Research Center (R13-2007-020-01000-0) at Chungnam National University. The authors declare that they have no competing financial interests.


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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Hye-Mi Lee
    • 1
    • 2
  • Dong-Min Shin
    • 1
    • 2
  • Kwang-Kyu Kim
    • 1
    • 2
  • Ji-Sook Lee
    • 2
    • 3
  • Tae-Hyun Paik
    • 3
  • Eun-Kyeong Jo
    • 1
    • 2
  1. 1.Department of Microbiology, College of MedicineChungnam National UniversityDaejeonSouth Korea
  2. 2.Infection Signaling Network Research Center, College of MedicineChungnam National UniversityDaejeonSouth Korea
  3. 3.Department of Microbiology, College of MedicineKonyang UniversityDaejeonSouth Korea

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