Journal of Clinical Immunology

, Volume 28, Issue 2, pp 147–156

Diesel Exhaust Particle-Exposed Human Bronchial Epithelial Cells Induce Dendritic Cell Maturation and Polarization via Thymic Stromal Lymphopoietin

  • Bertram Bleck
  • Doris B. Tse
  • Maria A. Curotto de Lafaille
  • Feijie Zhang
  • Joan Reibman
Article

Abstract

Human exposure to air pollutants, including ambient particulate matter, has been proposed as a mechanism for the rise in allergic disorders. Diesel exhaust particles, a major component of ambient particulate matter, induce sensitization to neoallergens, but the mechanisms by which sensitization occur remain unclear. We show that diesel exhaust particles upregulate thymic stromal lymphopoietin in human bronchial epithelial cells in an oxidant-dependent manner. Thymic stromal lymphopoietin induced by diesel exhaust particles was associated with maturation of myeloid dendritic cells, which was blocked by anti-thymic stromal lymphopoietin antibodies or silencing epithelial cell-derived thymic stromal lymphopoietin. Dendritic cells exposed to diesel exhaust particle-treated human bronchial epithelial cells induced Th2 polarization in a thymic stromal lymphopoietin-dependent manner. These findings provide new insight into the mechanisms by which diesel exhaust particles modify human lung mucosal immunity.

Keywords

Dendritic cells bronchial epithelial cells diesel exhaust particles thymic stromal lymphopoietin lung 

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Bertram Bleck
    • 1
  • Doris B. Tse
    • 1
    • 2
  • Maria A. Curotto de Lafaille
    • 3
    • 4
  • Feijie Zhang
    • 1
  • Joan Reibman
    • 1
    • 5
  1. 1.Department of Medicine, Division of Pulmonary and Critical Care MedicineNew York University School of MedicineNew YorkUSA
  2. 2.Division of Infectious Diseases, Department of MedicineNew York University School of MedicineNew YorkUSA
  3. 3.Department of Pathology, Molecular Pathogenesis ProgramNew York University School of MedicineNew YorkUSA
  4. 4.Skirball Institute for Biomolecular MedicineNew York University School of MedicineNew YorkUSA
  5. 5.Department of Medicine and Environmental MedicineNew York University School of MedicineNew YorkUSA

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