Journal of Clinical Immunology

, Volume 26, Issue 4, pp 308–322

RETRACTED ARTICLE: Oleandrin-Mediated Expression of Fas Potentiates Apoptosis in Tumor Cells

  • YASHIN SREENIVASAN
  • PONGALI B. RAGHAVENDRA
  • SUNIL K. MANNA
Article

DOI: 10.1007/s10875-006-9028-0

Cite this article as:
SREENIVASAN, Y., RAGHAVENDRA, P.B. & MANNA, S.K. J Clin Immunol (2006) 26: 308. doi:10.1007/s10875-006-9028-0

Chemotherapeutic agent is characterized by its concentration in tumor cells with minimum side effects. Oleandrin, a polyphenolic cardiac glycoside is known to induce apoptosis in tumor cells. However, no report is available on its efficacy in primary cells. In this report we are providing the evidence that oleandrin induces apoptosis, not necrosis in tumor cells but not in primary cells like peripheral blood mononuclear cells (PBMC) and neutrophils. Oleandrin inhibited NF-κB activation in tumor cells but not in primary cells. It induced cell death in NF-κB-overexpressed tumor cells. Oleandrin induced Fas expression thereby inducing apoptosis in tumor cells but not in primary cells. Dominant negative FADD inhibited oleandrin-induced cell death in tumor cells. Overall, these results suggest that oleandrin mediates apoptosis in tumor cells by inducing Fas but not in primary cells indicating its potential anti-cancer property with no or slight side effect.

KEY WORDS:

NF-κB Oleandrin Fas Apoptosis FADD 

Abbreviations used:

FADD

Fas-associated death domain

GFP

green fluorescent peptide

IκBα

inhibitory subunit of NF-κB

IκBα-DN

IκBα dominant negative

LDH

lactate dehydrogenase

MDA

malondialdehyde

NF-κB

nuclear transcription factor kappa B

MTT

3-(4,5-dimethyl-2-thiozolyl)-2,5-diphenyl-2H-tetrazolium bromide

PARP

poly-ADP ribose polymerase

PBMC

peripheral blood mononuclear cells

PIS

preimmune serum

ROI

reactive oxygen intermediates

SA-LPS

serum-activated lipopolysaccharide

SEAP

secretory alkaline phosphatase.

Copyright information

© Springer Science Business Media, Inc. 2006

Authors and Affiliations

  • YASHIN SREENIVASAN
    • 1
  • PONGALI B. RAGHAVENDRA
    • 1
  • SUNIL K. MANNA
    • 1
    • 2
  1. 1.Laboratory of ImmunologyCentre for DNA Fingerprinting & Diagnostics (CDFD)NacharamHyderabadIndia
  2. 2.Laboratory of ImmunologyCentre for DNA Fingerprinting & Diagnostics (CDFD)NacharamHyderabadIndia

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