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High diversity of haptophytes in the East China Sea revealed by next-generation sequencing and scanning electron microscopy

  • Chi-Yu Shih
  • Hsing-Ming Lu
  • Gwo-Ching Gong
  • Lee-Kuo KangEmail author
Original Article
  • 55 Downloads

Abstract

Haptophytes are a major marine microbial eukaryote group and play important roles in the marine ecosystem. To explore the phylogenetic diversity in haptophyte communities at high sequencing depths, we performed next-generation sequencing (NGS) to investigate the haptophyte community diversity in the East China Sea (ECS) during the summer of 2013. After clustering at 99% similarity, a total of 159 unique operational taxonomic units were detected. All major haptophyte groups, including several novel environmental lineages, were retrieved in the ECS. Chrysochromulinaceae and Phaeocystales represented the most important components of the haptophyte summer community. Our results indicated the existence of high haptophyte diversity and novel haptophyte lineages in the ECS. A comparison between the results from NGS and scanning electron microscopy (SEM) with respect to species diversity and the proportional abundance of coccolithophores showed that NGS had the power to explore overall community diversity, and SEM was capable of a high level of taxonomic resolution to distinguish closely related species with similar 18S rDNA sequences. The NGS method combined with the SEM method could provide comprehensive information for understanding the abundance and distribution of haptophytes in the ocean.

Keywords

Haptophytes Coccolithophores 18S rDNA Phylogeny Richness East China Sea Next-generation sequencing Scanning electron microscopy 

Notes

Acknowledgements

We are grateful to the captain and crew of the R/V Ocean Researcher I. This research was supported by the Ministry of Science and Technology Grant of MOST 107-2611-M-019-009. C.-Y. Shih was supported by a MOST postdoctoral fellowship (MOST 106-2811-M-019-001).

Supplementary material

10872_2019_505_MOESM1_ESM.xls (34 kb)
Supplementary material 1 (XLS 34 kb)
10872_2019_505_MOESM2_ESM.xlsx (27 kb)
Supplementary material 2 (XLSX 27 kb)
10872_2019_505_MOESM3_ESM.pdf (677 kb)
Supplementary material 3 (PDF 677 kb)

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© The Oceanographic Society of Japan and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Marine BiologyNational Taiwan Ocean UniversityKeelungTaiwan, ROC
  2. 2.Institute of Marine Environment and EcologyNational Taiwan Ocean UniversityKeelungTaiwan, ROC
  3. 3.Center of Excellence for the OceansNational Taiwan Ocean UniversityKeelungTaiwan, ROC
  4. 4.Bachelor Degree Program in Marine BiotechnologyNational Taiwan Ocean UniversityKeelungTaiwan, ROC

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