Journal of Oceanography

, Volume 74, Issue 2, pp 197–207 | Cite as

Habitability analyses of aquatic bacteria

  • Md. Nurul Haider
  • Masahiko Nishimura
  • Minoru Ijichi
  • Ching-chia Yang
  • Wataru Iwasaki
  • Kazuhiro Kogure
Original Article


Habitability is defined as an ability of an organism to inhabit different environments. Habitability of organisms, however, cannot be inferred from analyses such as a whole genome or community structures. A recently developed database, the MetaMetaDB, gives us information from what kind of environments one particular 16S rRNA sequence data has ever been obtained, and thus enables us to infer the habitability of the bacterium in question. In order to check the applicability of this database to study the habitability of aquatic bacteria, samples collected at two Naka River stations, one estuarine station from Naka River Estuary, two coastal stations at Oarai in Ibaraki Prefecture, Japan and one station in the Kuroshio Current of the western North Pacific were examined. The phylotypes were tracked against the MetaMetaDB and it was reasonably found that the low-salinity stations were dominated by sequences with “freshwater and groundwater”, “human” and “wastewater” habitat identities, while the high-salinity stations were dominated by those with a “marine” identity. The phylotypes of low-salinity stations with a particular habitat identity were absent or rare in the high-salinity stations and vice versa. The MetaMetaDB also showed that sequences of Cyanobacteria or related phylogenetic groups may be present in the human gut, as well as the probable distribution of the relatives (ancestors/descendants/siblings) of some bacteria. These overall findings proved that the MetaMetaDB is useful as a new tool to infer microbial habitability and it gives us new information on the possible origin and ecology of microorganisms in the environments.


16S rRNA 454 Pyrosequencing MetaMetaDB Habitability Aquatic bacteria 

Supplementary material

10872_2017_449_MOESM1_ESM.docx (391 kb)
Supplementary material 1 (DOCX 391 kb)
10872_2017_449_MOESM2_ESM.docx (71 kb)
Supplementary material 2 (DOCX 70 kb)


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Copyright information

© The Oceanographic Society of Japan and Springer Japan KK 2017

Authors and Affiliations

  1. 1.Atmosphere and Ocean Research InstituteThe University of TokyoKashiwaJapan
  2. 2.Department of Biological Sciences, Graduate School of ScienceThe University of TokyoTokyoJapan
  3. 3.Faculty of FisheriesBangladesh Agricultural UniversityMymensinghBangladesh

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