Advertisement

Journal of Oceanography

, Volume 65, Issue 6, pp 835–846 | Cite as

Variation of dissolved organic matter and fluorescence characteristics before, during and after phytoplankton bloom

  • Marut Suksomjit
  • Seiya Nagao
  • Kazuhiko Ichimi
  • Tatsuo Yamada
  • Kuninao TadaEmail author
Original Articles

Abstract

The variation of dissolved organic matter (DOM) and fluorescence characteristics during the phytoplankton bloom were investigated in Yashima Bay, at the eastern part of the Seto Inland Sea, Japan. We found significant accumulations of dissolved organic carbon (DOC), dissolved organic nitrogen (DON), chromophoric dissolved organic matter (CDOM) fluorescence, and UV260 during the phytoplankton bloom period in 2005, although lower accumulations of DOC and DON and only increases of CDOM fluorescence were observed during the bloom period in 2006. Little or no correlation between DOM and phytoplankton abundance might be due to the composition of DOM, which is a complex mixture of organic materials. The 3D-EEM results revealed that the DOM produced around the phytoplankton bloom period contained tyrosine, tryptophan, and humic-like substances. Our results showed that the occurrence of phytoplankton bloom contributed to the production of DOM in coastal water but the DOM accumulation depended on the type of phytoplankton bloom, the phytoplankton species in particular. From our results, we concluded that phytoplankton have a great role in the dynamics of DOM as a producer in a coastal environment.

Keywords

DOM DOC DON 3D-EEM phytoplankton bloom 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Anderson, P. and T. Fenchel (1985): Bacterivory by microheterotrophic flagellates in seawater samples. Limnol. Oceanogr., 30, 198–202.CrossRefGoogle Scholar
  2. Azam, F., T. Fenchel, J. G. Field, J. S. Gray, L. A. Meyer-Reil and F. Thingstad (1983): The ecological role of water-column microbes in the Sea. Mar. Ecol. Prog. Ser., 10, 257–263.CrossRefGoogle Scholar
  3. Biddanda, B. and R. Benner (1997): Carbon, nitrogen, and carbohydrate fluxes during the production of particulate and dissolved organic matter by marine phytoplankton. Limnol. Oceanogr., 42, 506–518.CrossRefGoogle Scholar
  4. Bronk, D. A. (2002): Dynamics of DON. p. 153–247. In Biogeochemistry of Marine Dissolved Organic Matter, ed. by D. A. Hansell and C. A. Carlson, Academic Press, U.S.A.CrossRefGoogle Scholar
  5. Coble, P. G. (1996): Characterization of marine and terrestrial DOM in seawater using excitation-emission matrix spectroscopy. Mar. Chem., 51, 325–346.CrossRefGoogle Scholar
  6. Coble, P. G., C. E. D. Castillo and B. Avril (1998): Distribution and optical properties of CDOM in the Arabian Sea during the 1995 Southwest Monsoon. Deep-Sea Res. II., 45, 2195–2223.CrossRefGoogle Scholar
  7. Conan, P., M. Søndergaard, T. Kragh, F. Thingstad, M. Pujo-Pay, P. J. le B. Williams, S. Markager, G. Cauwet, N. H. Borch, D. Evans and B. Riemann (2007): Partitioning of organic production in marine plankton communities: the effects of inorganic nutrient ratios and community composition on new dissolved organic matter. Limnol. Oceanogr., 52, 753–765.CrossRefGoogle Scholar
  8. Dilling, J. and K. Kaiser (2002): Estimation of the hydrophobic fraction of dissolved organic matter in water samples using UV photometry. Water Res., 36, 5037–5044.CrossRefGoogle Scholar
  9. Eppley, R. W. and B. J. Peterson (1979): Particulate organic matter flux and planktonic new production in the deep ocean. Nature, 282, 677–680.CrossRefGoogle Scholar
  10. Hansell, D. A. and C. A. Carlson (2002): Biogeochemistry of Marine Dissolved Organic Matter. Academic Press, U.S.A., 774 pp.Google Scholar
  11. Hobbie, J. E., R. J. Daley and S. Jasper (1997): Use of nucleopore filters for counting bacteria by fluorescence microscopy. Appl. Environ. Microbial., 33, 1225–1228.Google Scholar
  12. Holm-Hansen, O., C. J. Lorenzen, R. W. Holmes and J. D. Strickland (1965): Fluorometric determination of chlorophyll. J. Cons. Perm. Int. Explor. Mer., 30, 3–15.CrossRefGoogle Scholar
  13. Honjo, T. (2003): Heterosigma akashiwo (Red-tide species and the environmental conditions). p. 333–344. In Red Tides, ed. by T. Okaichi, Terra Scientific Publishing Company, Tokyo.Google Scholar
  14. Hudson, N., A. Baker and D. Reynolds (2007): Fluorescence analysis of dissolved organic matter in natural, waste and polluted waters-a review. River Res. Applic., 23, 631–649.CrossRefGoogle Scholar
  15. Kamiyama, T., S. Itakura and K. Nagasaki (2000): Changes in microbial loop components: Effects of a harmful algal bloom formation and its decay. Aquat. Micro. Ecol., 21, 21–30.CrossRefGoogle Scholar
  16. Lønborg, C., K. Davidson, X. A. Álvarez-Salgado and A. E. J. Miller (2009): Bioavailability and bacterial degradation rates of dissolved organic matter in a temperate coastal area during an annual cycle. Mar. Chem., doi:10.1016/j.marchem.2009.02.003.CrossRefGoogle Scholar
  17. Lorenzo, J. I., M. Nieto-Cid, X. A. Álvarez-Salgado, P. Pérez and R. Beiras (2007): Contrasting complexing capacity of dissolved organic matter produced during the onset, development and decay of a simulated bloom of the marine diatom Skeletonema costatum. Mar. Chem., 103, 61–75.CrossRefGoogle Scholar
  18. Mague, T. H., E. Friberg, D. J. Hughes and I. Morris (1980): Extracellular release of carbon by marine phytoplankton; physiological approach. Limnol. Oceanogr., 25, 262–279.CrossRefGoogle Scholar
  19. Mattson, J. S., C. A. Smith, T. T. Jones, S. M. Gerchakov and B. D. Epstein (1974): Continuous monitoring of dissolved organic matter by ultraviolet-visible photometry. Limnol. Oceanogr., 19, 530–535.CrossRefGoogle Scholar
  20. Minor, E. C., J. Simjouw and M. R. Mulholland (2006): Seasonal variations in dissolved organic carbon concentrations and characteristics in a shallow coastal bay. Mar. Chem., 101, 166–179.CrossRefGoogle Scholar
  21. Nagao, S., Y. Suzuki, Y. Nakaguchi, M. Senoo and K. Hiraki (1997): Direct measurement of the fluorescence characteristics of aquatic humic substances by a three-dimensional fluorescence spectrometer. Bunseki Kagaku, 46, 335–342 (in Japanese with English abstract).CrossRefGoogle Scholar
  22. Nagao, S., T. Matsunaga, Y. Suzuki and K. Hiraki (2001): Convenient method of UV-absorbing organic materials in river waters by high-performance gel permeation chromatography. Chikyu-Kagaku, 35, 107–120 (in Japanese with English abstract).Google Scholar
  23. Nagao, S., T. Matsunaga, Y. Suzuki, T. Ueno and H. Amano (2003): Characteristics of humic substances in the Kuji River waters as determined by high-performance size exclusion chromatography with fluorescence detection. Water Res., 37, 4159–4170.CrossRefGoogle Scholar
  24. Nagata, T. (2000): Production mechanisms of dissolved organic matter. p. 121–152. In Microbial Ecology of the Oceans, ed. by D. L. Kirchman, Willey-Liss, New York.Google Scholar
  25. Nelson, N. B., D. A. Siegel and A. F. Michaels (1998): Seasonal dynamics of colored dissolved material in the Sargasso Sea. Deep-Sea Res. I., 45, 931–957.CrossRefGoogle Scholar
  26. Ogawa, H. and E. Tanoue (2003): Dissolved organic matter in oceanic waters. J. Oceanogr., 59, 129–147.CrossRefGoogle Scholar
  27. Ogawa, H., Y. Amagai, I. Koike, K. Kaiser and R. Benner (2001): Production of refractory dissolved organic matter by bacteria. Science, 292, 917–920.CrossRefGoogle Scholar
  28. Saadi, I., M. Borisovor, R. Armon and Y. Laor (2006): Monitoring of effluent DOM biodegradation using fluorescence, UV and DOC measurements. Chemo., 63, 530–539.CrossRefGoogle Scholar
  29. Sasaki, H., T. Miyamura, S. Saitoh and J. Ishizaka (2005): Seasonal variation of absorption of particles and colored dissolved organic matter (CDOM) in Funka Bay, southwestern Hokkaido, Japan. Estuar. Coast. Shelf Sci., 64, 447–458.CrossRefGoogle Scholar
  30. Smart, P. L., B. L. Finlayson, W. D. Rylands and C. M. Ball (1976): The relation of fluorescence to dissolved organic carbon in surface waters. Water Res., 10, 805–811.CrossRefGoogle Scholar
  31. Solózano, L. and J. H. Sharp (1980): Determination of total dissolved phosphorus and particulate phosphorus in natural waters. Limnol. Oceanogr., 25, 754–758.CrossRefGoogle Scholar
  32. Stedmon, C. A. and S. Markager (2005): Tracing the production and degradation of autochthonous fractions of dissolved organic matter by fluorescence analysis. Limnol. Oceanogr., 50, 1415–1426.CrossRefGoogle Scholar
  33. Strickland, J. D. H. and T. R. Parsons (1972): A Practical Handbook of Seawater Analysis (2nd). Fish. Res. Boar. Can. Bull., 167, 311 pp.Google Scholar
  34. Van den Meersche, K., J. J. Middelburg, K. Soetaert, P. Van Rijswijk, H. T. S. Boschker and C. H. R. Heip (2004): Carbon-nitrogen coupling and algal-bacterial interactions during an experimental bloom: Modeling a 13C tracer experiment. Limnol. Oceanogr., 49, 862–878.CrossRefGoogle Scholar
  35. Wetz, M. S. and P. A. Wheeler (2007): Release of dissolved organic matter by coastal diatoms. Limnol. Oceanogr., 52, 798–807.CrossRefGoogle Scholar
  36. Yamada, T. (1984): Study on the pollution load of Yashima Bay. Sci. Rep. Kagawa Pref. Fish. Exp. Stn., 21, 1–9 (in Japanese).Google Scholar
  37. Yamamoto, T., Y. Inokuchi and T. Sugiyama (2004): Biogeochemical cycles during the species succession from Skeletonema costatum and Alexandrium tamarense in northern Hiroshima Bay. J. Mar. Sys., 52, 15–32.CrossRefGoogle Scholar
  38. Yamashita, Y. and E. Tanoue (2003): Chemical characterization of protein-like fluorescences in DOM in relation to aromatic amino acids. Mar. Chem., 82, 255–271.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Marut Suksomjit
    • 1
  • Seiya Nagao
    • 2
  • Kazuhiko Ichimi
    • 3
  • Tatsuo Yamada
    • 4
  • Kuninao Tada
    • 1
    Email author
  1. 1.Faculty of AgricultureKagawa UniversityKagawaJapan
  2. 2.Low Level Radioactivity Laboratory, KU-INETKanazawa UniversityWake, Nomi, IshikawaJapan
  3. 3.Aji Marine Station, Seto Inland Sea Regional Research CenterKagawa UniversityAji, Takamatsu, KagawaJapan
  4. 4.Kagawa Prefectural Fisheries Experimental StationTakamatsu, KagawaJapan

Personalised recommendations