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Journal of Biological Physics

, Volume 39, Issue 1, pp 1–14 | Cite as

Anomalies in the motion dynamics of long-flagella mutants of Chlamydomonas reinhardtii

  • Dolly K. Khona
  • Venkatramanan G. Rao
  • Mustafa J. Motiwalla
  • P. C. Sreekrishna Varma
  • Anisha R. Kashyap
  • Koyel Das
  • Seema M. Shirolikar
  • Lalit Borde
  • Jayashree A. Dharmadhikari
  • Aditya K. Dharmadhikari
  • Siuli Mukhopadhyay
  • Deepak Mathur
  • Jacinta S. D’SouzaEmail author
Original Paper

Abstract

Chlamydomonas reinhardtii has long been used as a model organism in studies of cell motility and flagellar dynamics. The motility of the well-conserved ‘9+2’ axoneme in its flagella remains a subject of immense curiosity. Using high-speed videography and morphological analyses, we have characterized long-flagella mutants (lf1, lf2-1, lf2-5, lf3-2, and lf4) of C. reinhardtii for biophysical parameters such as swimming velocities, waveforms, beat frequencies, and swimming trajectories. These mutants are aberrant in proteins involved in the regulation of flagellar length and bring about a phenotypic increase in this length. Our results reveal that the flagellar beat frequency and swimming velocity are negatively correlated with the length of the flagella. When compared to the wild-type, any increase in the flagellar length reduces both the swimming velocities (by 26–57%) and beat frequencies (by 8–16%). We demonstrate that with no apparent aberrations/ultrastructural deformities in the mutant axonemes, it is this increased length that has a critical role to play in the motion dynamics of C. reinhardtii cells, and, provided there are no significant changes in their flagellar proteome, any increase in this length compromises the swimming velocity either by reduction of the beat frequency or by an alteration in the waveform of the flagella.

Keywords

Chlamydomonas reinhardtii Flagella Motion dynamics Swimming velocity Beat frequency Waveforms Long-flagella mutants Motility Dynein arms 

Notes

Acknowledgements

The Inter-Academy Summer Students program supported Anisha R. Kashyap. We thank P. A. Lefebvre for kindly donating the lf4 mutant cells and Krishanu Ray for permitting use of the TEM facility at the Tata Institute of Fundamental Research. We also acknowledge valuable input provided by David Mitchell (Upstate Medical University, USA) for standardizing the conditions for the TEM of axonemes.

Supplementary material

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Dolly K. Khona
    • 1
  • Venkatramanan G. Rao
    • 1
  • Mustafa J. Motiwalla
    • 1
  • P. C. Sreekrishna Varma
    • 1
  • Anisha R. Kashyap
    • 2
  • Koyel Das
    • 3
  • Seema M. Shirolikar
    • 5
  • Lalit Borde
    • 5
  • Jayashree A. Dharmadhikari
    • 4
  • Aditya K. Dharmadhikari
    • 4
  • Siuli Mukhopadhyay
    • 3
  • Deepak Mathur
    • 4
  • Jacinta S. D’Souza
    • 1
    Email author
  1. 1.UM-DAE-Centre for Excellence in Basic SciencesBiological SciencesMumbaiIndia
  2. 2.K. J. Somaiya College of Commerce and ScienceMumbaiIndia
  3. 3.Department of MathematicsIndian Institute of Technology BombayMumbaiIndia
  4. 4.Tata Institute of Fundamental ResearchMumbaiIndia
  5. 5.Department of Biological SciencesTata Institute of Fundamental ResearchMumbaiIndia

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