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Journal of Biological Physics

, Volume 38, Issue 1, pp 61–74 | Cite as

New insights into bioprotective effectiveness of disaccharides: an FTIR study of human haemoglobin aqueous solutions exposed to static magnetic fields

  • Salvatore MagazùEmail author
  • Emanuele Calabrò
  • Salvatore Campo
  • Salvatore Interdonato
Original Paper

Abstract

The aim of this study was the investigation of static magnetic field effects on haemoglobin secondary structure and the bioprotective effectiveness of two disaccharides, sucrose and trehalose. Samples of haemoglobin aqueous solutions, in the absence and in the presence of sucrose and trehalose, were exposed to a uniform magnetic field at 200 mT, which is the exposure limit established by the ICNIRP recommendation for occupational exposure. Spectral analysis by FTIR spectroscopy after 3 and 7 h of exposure revealed a decrease in the amide A vibration band for haemoglobin in bi-distilled water solution. Analogue exposures did not produce any appreciable change of amide A for haemoglobin in sucrose and trehalose solutions. Otherwise, no relative increase of \(\upbeta \)-sheet contents in amide I and II regions was detected for haemoglobin aqueous solutions, leading us to exclude the hypothesis that static magnetic fields can induce the formation of aggregates in the protein. In addition, a decrease in CH3 stretching linkages occurred for haemoglobin in bi-distilled water solution after exposure, which was not observed for haemoglobin in sucrose and trehalose aqueous solutions, providing further evidence of a bioprotective compensatory mechanism of such disaccharides.

Keywords

Haemoglobin Static magnetic field Electromagnetic field Infrared spectroscopy Disaccharides Trehalose 

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Salvatore Magazù
    • 1
    Email author
  • Emanuele Calabrò
    • 1
  • Salvatore Campo
    • 2
  • Salvatore Interdonato
    • 1
  1. 1.Department of PhysicsUniversity of MessinaMessinaItaly
  2. 2.Department of Biochemical, Physiological and Nutritional SciencesUniversity of MessinaMessinaItaly

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