Bacterium in a box: sensing of quorum and environment by the LuxI/LuxR gene regulatory circuit
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The chemical signaling mechanism known as “bacterial quorum sensing” (QS) is normally interpreted as allowing bacteria to detect their own population density, in order to coordinate gene expression across a colony. However, the release of the chemical signal can also be interpreted as a means for one or a few cells to probe the local physical properties of their microenvironment. We have studied the behavior of the LuxI/LuxR QS circuit of Vibrio fischeri in tightly confining environments where individual cells detect their own released signals. We find that the lux genes become activated in these environments, although the activation onset time shows substantial cell-to-cell variability and little sensitivity to the confining volume. Our data suggest that noise in gene expression could significantly impact the utility of LuxI/LuxR as a probe of the local physical environment.
KeywordsQuorum sensing Noise Microenvironments Fluorescence GFP Diffusion Bacteria LuxIR Stochastic Bioluminescence Autoinduction Vibrio fischeri
The authors thank Drs. Mark Mandel, Ned Ruby, Rena Hill, and Christopher Voigt for providing bacterial strains used in this study. The authors also gratefully acknowledge funding support from the National Science Foundation MCB no. 0347124 and the National Institutes of Health NIDCR 1R21DE018826.
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