Efficiency of Resonance Energy Transfer in Homo-Oligomeric Complexes of Proteins
- 251 Downloads
A theoretical model is proposed for the apparent efficiency of fluorescence (Förster) resonance energy transfer (FRET) in mixtures of free monomers and homo-oligomeric protein complexes of uniform size. The model takes into account possible pathways for transfer of optical excitations from single donors to multiple acceptors and from multiple donors (non-simultaneously) to single acceptors. This necessary departure from the standard theory has been suggested in the literature, but it has only been successfully implemented for a few particular cases, such as for particular geometries of the oligomers. The predictions of the present theoretical model differ significantly from those of the standard theory, with the exception of the case of dimers, for which agreement is observed. This model therefore provides new insights into the FRET behavior of oligomers comprising more than two monomers, and also suggests means for determining the size of oligomeric protein complexes as well as the proportion of associated and unassociated monomers.
KeywordsFörster (fluorescence) resonance energy transfer FRET Fluorescence theory Protein – protein interaction Protein association Protein self-association Interaction stoichiometry
Unable to display preview. Download preview PDF.
- 1.Gomperts, B.D., Kramer, I.M., Tatham, P.E.R.: Signal Transduction. Academic, New York (2002)Google Scholar
- 7.Lakowicz, J.R.: Principles of Fluorescence Spectroscopy. 3rd edn. Springer, Berlin (2006)Google Scholar
- 8.Clegg, R.M.: Fluorescence resonance energy transfer. In: Fluorescence Imaging Spectroscopy and Microscopy, Wang, X.F. and Herman, B. (eds). Wiley, New York (1996)Google Scholar
- 11.Li, M., Reddy, L.G., Bennett, R., Silva, N.D., Jones, L.R., Thomas, D.D.: A fluorescence energy transfer method for analyzing protein oligomeric structure: application to phospholamban. Biophys. J. 76, 2587–2599 (1999)Google Scholar
- 12.Zacharias, D.A., Baird, G.S., Tsien, R.Y.: Recent advances in technology for measuring and manipulating cell signals. Curr. Opin. Cell Biol. 10, 416–421 (2000)Google Scholar
- 13.Kenworthy, A.K., Petranova, N., Edidin, M.: High-resolution FRET microscopy of cholera toxin B-subunit and GPI-anchored proteins in cell plasma membranes. Mol. Biol. Cell. 11(5), 1645–1655 (2000)Google Scholar
- 19.Karpova, T.S., Baumann, C.T., He, L., Wu, X., Grammer, A., Lipsky, P., Hager, G.L., McNally, J.G.: Fluorescence resonance energy transfer from cyan to yellow fluorescent protein detected by acceptor photobleaching using confocal microscopy and a single laser. J. Microsc. 209(1), 56–70 (2003)CrossRefMathSciNetGoogle Scholar