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Journal of Bioenergetics and Biomembranes

, Volume 49, Issue 2, pp 149–158 | Cite as

Energetic, oxidative and ionic exchange in rat brain and liver mitochondria at experimental audiogenic epilepsy (Krushinsky–Molodkina model)

  • Natalya I. VenediktovaEmail author
  • Olga S. Gorbacheva
  • Natalia V. Belosludtseva
  • Irina B. Fedotova
  • Natalia M. Surina
  • Inga I. Poletaeva
  • Oleg V. Kolomytkin
  • Galina D. Mironova
Article

Abstract

The role of brain and liver mitochondria at epileptic seizure was studied on Krushinsky-Molodkina (KM) rats which respond to sound with an intensive epileptic seizure (audiogenic epilepsy). We didn't find significant changes in respiration rats of brain and liver mitochondria of KM and control rats; however the efficiency of АТР synthesis in the KM rat mitochondria was 10% lower. In rats with audiogenic epilepsy the concentration of oxidative stress marker malondialdehyde in mitochondria of the brain (but not liver) was 2-fold higher than that in the control rats. The rate of H2O2 generation in brain mitochondria of КМ rats was twofold higher than in the control animals when using NAD-dependent substrates. This difference was less pronounced in liver mitochondria. In KM rats, the activity of mitochondrial ATP-dependent potassium channel was lower than in liver mitochondria of control rats. The comparative study of the mitochondria ability to retain calcium ions revealed that in the case of using the complex I and complex II substrates, permeability transition pore is easier to trigger in brain and liver mitochondria of KM and КМs rats than in the control ones. The role of the changes in the energetic, oxidative, and ionic exchange in the mechanism of audiogenic epilepsy generation in rats and the possible correction of the epilepsy seizures are discussed.

Keywords

Audiogenic epilepsy mitoKATP Respiration Ions transport H2O2 production 

Notes

Acknowledgements

We are sincerely grateful to Dr. M.E. Astashev for providing the Filter software package for the curve smoothing. This work was supported by Russian Science foundation (RSF) (grant № 16-15-00157) (http://xn--m1afn.xn--p1ai/) (Mironova G.D., Belosludtseva N.V., Gorbacheva O.S.); the work with experimental animals, procedure of their sonication and the work of technicians were supported by Russian Foundation for Basic Research (RFBR) (grants № 04-15-01732a and 16-04-00692a).

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Natalya I. Venediktova
    • 1
    Email author
  • Olga S. Gorbacheva
    • 1
  • Natalia V. Belosludtseva
    • 1
  • Irina B. Fedotova
    • 2
  • Natalia M. Surina
    • 2
  • Inga I. Poletaeva
    • 2
  • Oleg V. Kolomytkin
    • 1
  • Galina D. Mironova
    • 1
  1. 1.Institute of Theoretical and Experimental Biophysics RASMoscow RegionRussia
  2. 2.Biology Department, Laboratory for Physiology and Genetics of BehaviorLomonosov Moscow State UniversityMoscowRussia

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