Journal of Bioenergetics and Biomembranes

, Volume 47, Issue 4, pp 331–336 | Cite as

Resveratrol increases glycolytic flux in Saccharomyces cerevisiae via a SNF1-dependet mechanism

  • Luis Alberto Madrigal-Perez
  • Gerardo M. Nava
  • Juan Carlos González-Hernández
  • Minerva Ramos-Gomez


Evidence suggests that AMP protein kinase (AMPK) is the main target of the phytochemical resveratrol (RSV) in mammalian cells. Data also indicates that RSV stimulates glucose metabolism; however, the molecular link between RSV and glucose uptake remains unknown. Herein, we provide evidence indicating that RSV stimulates glycolysis via sucrose non-fermenting 1 gene (SNF1, Saccharomyces cerevisiae orthologous of AMPK). S. cerevisiae cultures treated with 30 μM RSV showed an increase in extracellular acidification rate compared to untreated cells, indicating an elevated glycolytic flux. Also, RSV treatment increased transcription levels of two key glycolytic genes, hexokinase 2 (HXK2) and phosphofructokinase 1 (PFK1), as well as production of NADH. Moreover, RSV treatment inhibited mitochondrial respiration when glucose was used as a carbon source. Importantly, the effects of RSV on glycolysis were dependent of SNF1. Taken together, these findings suggest that SNF1 (AMPK in mammalian systems) is the molecular target of RSV in S. cerevisiae.


Resveratrol Glucose metabolism Crabtree effect Respiration 



This work was financially supported by grants from Instituto Tecnológico Superior de Ciudad Hidalgo (3308.100310) and Universidad Autónoma de Querétaro (FCQ201417). The PROMEP program contributes with a scholarship grant for LAMP. The authors thank to Ana Karen Padilla-Pérez, Cecilia Martínez-Ortiz, Mayra Alejandra Soto-Villagómez, Josué Misael Zamudio-Bolaños, Andrés Carrillo-Garmendia and Maria Irene Cornelio-Martinez for the technical support in kinetic assays.

Conflict of Interest

The authors have no conflicts of interest to declare.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Luis Alberto Madrigal-Perez
    • 1
    • 3
  • Gerardo M. Nava
    • 3
  • Juan Carlos González-Hernández
    • 2
  • Minerva Ramos-Gomez
    • 3
  1. 1.Laboratorio de Biotecnología Microbiana del Instituto Tecnológico Superior de Ciudad HidalgoCiudad HidalgoMéxico
  2. 2.Laboratorio de Bioquímica del Instituto Tecnológico de MoreliaMoreliaMéxico
  3. 3.Universidad Autónoma de QuerétaroSantiago de QuerétaroMéxico

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